LEA32_ARATH
ID LEA32_ARATH Reviewed; 256 AA.
AC Q9LJ95; Q96245;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Late embryogenesis abundant protein 32 {ECO:0000303|PubMed:18318901};
DE Short=LEA 32 {ECO:0000303|PubMed:18318901};
DE AltName: Full=Embryogenic cell protein 31 {ECO:0000303|Ref.1};
DE Short=AtECP31 {ECO:0000303|Ref.1};
GN Name=ECP31 {ECO:0000303|Ref.1};
GN OrderedLocusNames=At3g22500 {ECO:0000312|Araport:AT3G22500};
GN ORFNames=F16J14.6 {ECO:0000312|EMBL:BAB01466.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAB01466.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia; TISSUE=Dry seed;
RX DOI=10.1111/j.1399-3054.1996.tb05724.x;
RA Yang H., Saitou T., Komeda Y., Harada H., Kamada H.;
RT "Late embryogenesis abundant protein in Arabidopsis thaliana homologous to
RT carrot ECP31.";
RL Physiol. Plantarum 98:661-666(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=10412913; DOI=10.1023/a:1006219315562;
RA Arenas-Mena C., Raynal M., Borrell A., Varoquaux F., Cutanda M.C.,
RA Stacy R.A.P., Pages M., Delseny M., Culianez-Macia F.A.;
RT "Expression and cellular localization of Atrab28 during arabidopsis
RT embryogenesis.";
RL Plant Mol. Biol. 40:355-363(1999).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18318901; DOI=10.1186/1471-2164-9-118;
RA Hundertmark M., Hincha D.K.;
RT "LEA (late embryogenesis abundant) proteins and their encoding genes in
RT Arabidopsis thaliana.";
RL BMC Genomics 9:118-118(2008).
RN [7]
RP SUBCELLULAR LOCATION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=25005920; DOI=10.1105/tpc.114.127316;
RA Candat A., Paszkiewicz G., Neveu M., Gautier R., Logan D.C.,
RA Avelange-Macherel M.-H., Macherel D.;
RT "The ubiquitous distribution of late embryogenesis abundant proteins across
RT cell compartments in Arabidopsis offers tailored protection against abiotic
RT stress.";
RL Plant Cell 26:3148-3166(2014).
CC -!- FUNCTION: LEA proteins are late embryonic proteins abundant in higher
CC plant seed embryos. The function of those proteins is not known.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25005920}. Nucleus
CC {ECO:0000269|PubMed:25005920}.
CC -!- TISSUE SPECIFICITY: Embryo specific, only in dry mature seeds.
CC Expressed at low levels. {ECO:0000269|PubMed:10412913,
CC ECO:0000269|Ref.1}.
CC -!- DEVELOPMENTAL STAGE: In mature embryos, restricted to provascular
CC tissues. Also present in the seed coat outer tegument and silique
CC epidermis. Levels decrease during seed imbibition and germination.
CC {ECO:0000269|PubMed:10412913}.
CC -!- SIMILARITY: Belongs to the LEA type SMP family. {ECO:0000305}.
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DR EMBL; D64139; BAA11016.1; -; mRNA.
DR EMBL; AP000731; BAB01466.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76647.1; -; Genomic_DNA.
DR EMBL; AY052316; AAK96509.1; -; mRNA.
DR EMBL; BT000845; AAN38682.1; -; mRNA.
DR RefSeq; NP_188889.1; NM_113149.4.
DR AlphaFoldDB; Q9LJ95; -.
DR STRING; 3702.AT3G22500.1; -.
DR PaxDb; Q9LJ95; -.
DR PRIDE; Q9LJ95; -.
DR ProMEX; Q9LJ95; -.
DR ProteomicsDB; 230198; -.
DR EnsemblPlants; AT3G22500.1; AT3G22500.1; AT3G22500.
DR GeneID; 821821; -.
DR Gramene; AT3G22500.1; AT3G22500.1; AT3G22500.
DR KEGG; ath:AT3G22500; -.
DR Araport; AT3G22500; -.
DR TAIR; locus:2076996; AT3G22500.
DR eggNOG; ENOG502QPSX; Eukaryota.
DR HOGENOM; CLU_075678_0_0_1; -.
DR InParanoid; Q9LJ95; -.
DR OMA; ETHVFGH; -.
DR OrthoDB; 1200778at2759; -.
DR PhylomeDB; Q9LJ95; -.
DR PRO; PR:Q9LJ95; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LJ95; baseline and differential.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR InterPro; IPR042971; LEA_SMP.
DR InterPro; IPR007011; LEA_SMP_dom.
DR PANTHER; PTHR31174; PTHR31174; 1.
DR Pfam; PF04927; SMP; 3.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat.
FT CHAIN 1..256
FT /note="Late embryogenesis abundant protein 32"
FT /id="PRO_0000436060"
FT DOMAIN 13..66
FT /note="SMP 1"
FT /evidence="ECO:0000255"
FT DOMAIN 130..187
FT /note="SMP 2"
FT /evidence="ECO:0000255"
FT DOMAIN 195..253
FT /note="SMP 3"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 5..9
FT /note="Nuclear localization signal (NLS)"
FT /evidence="ECO:0000250|UniProtKB:Q9LJ97"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 191
FT /note="D -> N (in Ref. 1; BAA11016)"
FT /evidence="ECO:0000305"
FT CONFLICT 254
FT /note="V -> F (in Ref. 1; BAA11016)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 256 AA; 26779 MW; A9E79894E2182F94 CRC64;
MSQEQPRRPR EPVKYGDVFE VSGELADKPI APEDAKMMQS AETHVFGHTQ KGGPAAVMQS
AATTNIRGGF VHPDDKTELV AERGATVEQT VPAATVTTEF VGGQVVGQHV EPRRVVAAAR
TDEEALQSTI TIGEALEATV KTAGNKPVDQ SDAAAIQAAE MRASGTNVIA LAGVAASAQS
AADHNATVDR DERKIKLRDV LTGAAGKLSA DRAVTREDAE GVVSAEMRNN PKLCTHPGGV
AASLTVAARL NERVDI
