ID   ARE2_SACU7              Reviewed;         650 AA.
AC   Q876L2;
DT   09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Sterol O-acyltransferase 2;
DE            EC=2.3.1.-;
DE   AltName: Full=Sterol-ester synthase 2;
GN   Name=ARE2;
OS   Saccharomyces uvarum (strain ATCC 76518 / CBS 7001 / CLIB 283 / NBRC 10550
OS   / MCYC 623 / NCYC 2669 / NRRL Y-11845) (Yeast) (Saccharomyces bayanus var.
OS   uvarum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=659244;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=623-6C / CBS 9787 / CLIB 533;
RX   PubMed=12594514; DOI=10.1038/nature01419;
RA   Langkjaer R.B., Cliften P.F., Johnston M., Piskur J.;
RT   "Yeast genome duplication was followed by asynchronous differentiation of
RT   duplicated genes.";
RL   Nature 421:848-852(2003).
CC   -!- FUNCTION: Sterol O-acyltransferase that catalyzes the formation of
CC       stery esters. {ECO:0000250|UniProtKB:P25628}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:P25628}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC       Sterol o-acyltransferase subfamily. {ECO:0000305}.
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DR   EMBL; AY144799; AAO32363.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q876L2; -.
DR   SMR; Q876L2; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008374; F:O-acyltransferase activity; IEA:InterPro.
DR   InterPro; IPR004299; MBOAT_fam.
DR   InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR   PANTHER; PTHR10408; PTHR10408; 1.
DR   Pfam; PF03062; MBOAT; 1.
DR   PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Endoplasmic reticulum; Membrane; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..650
FT                   /note="Sterol O-acyltransferase 2"
FT                   /id="PRO_0000207650"
FT   TRANSMEM        223..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        300..320
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        412..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        450..470
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        493..513
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        575..595
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        630..650
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          41..79
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           531..537
FT                   /note="FYXDWWN motif"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   ACT_SITE        587
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
SQ   SEQUENCE   650 AA;  74610 MW;  2F1B6654C6D9BC86 CRC64;
     MDKNKDLLEN EQFLRIQKLN ASDAGKRQSI LVDNEDELYG LTSSNNSCAS EHEGEGEGED
     ERPATTSSAP TQNHSAGDVA FIPGKTAEED TETVTKVVES DDQVFRTHVQ TLSSKGKSRY
     RKGSSNFISF FDDMAFENRP SILDGSVNDP FKTKFVGPTL EKEIRKREKE LMAMRKNLHH
     GKPAPDADAA DAPALTTTTT TTTSATSPET VVTIETTILS SNFSGLYVAF WMAIAFGAVK
     ALIDYYYQHN GSFKDSEILK FMTTNLSTVA LIDLIMYLST FFVVGIQYLC KWGVLNWSST
     GWAFTSIYEL LFVGFYMYLT ENILKLHWLS KIFLFLHSLV LLMKMHSFAF YNGYLWGIKQ
     ELQFSKSALA KYKDSVNDPD VVDALEKSCE FCSFELNSQS LNDQTQKFPN NINVSNFFMF
     TMFPTLIYQI EYPRTKEIRW VYVLEKICAI FGTIFLMMID AQILMHPVAM RALDVRNSEW
     TGILDRLLKW AGLLVDIVPG FIVMYILDFY LIWDAILNCV AELTRFGDRY FYGDWWNCVS
     WADFSRIWNI PVHKFLLRHV YHSSMSSFKL NKSQATLMTF FLSSVVHELA MYVIFKRLRF
     YLFFFQMLQV PLVALTNTKY MKDRTVIGNV IFWLGICMGP SVMCTLYLTF