LEA46_ARATH
ID LEA46_ARATH Reviewed; 158 AA.
AC Q9FG31; Q42177; Q96272;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Late embryogenesis abundant protein 46 {ECO:0000303|PubMed:18318901};
DE AltName: Full=Late embryogenesis abundant protein 4-5 {ECO:0000303|PubMed:20668063};
DE Short=AtLEA4-5 {ECO:0000303|PubMed:20668063};
GN Name=LEA46 {ECO:0000303|PubMed:18318901};
GN Synonyms=LEA4-5 {ECO:0000303|PubMed:20668063};
GN OrderedLocusNames=At5g06760 {ECO:0000312|Araport:AT5G06760};
GN ORFNames=MPH15.12 {ECO:0000312|EMBL:BAB09810.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Dry seed;
RA Raynal M.;
RT "A.thaliana mRNA for LEA D113 type 1 protein.";
RL Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-82.
RC STRAIN=cv. Columbia; TISSUE=Dry seed;
RX PubMed=8580968; DOI=10.1046/j.1365-313x.1996.09010101.x;
RA Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C.,
RA Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R.,
RA Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J.,
RA Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D.,
RA Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P.,
RA Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.;
RT "Further progress towards a catalogue of all Arabidopsis genes: analysis of
RT a set of 5000 non-redundant ESTs.";
RL Plant J. 9:101-124(1996).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18318901; DOI=10.1186/1471-2164-9-118;
RA Hundertmark M., Hincha D.K.;
RT "LEA (late embryogenesis abundant) proteins and their encoding genes in
RT Arabidopsis thaliana.";
RL BMC Genomics 9:118-118(2008).
RN [7]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=20668063; DOI=10.1104/pp.110.158964;
RA Olvera-Carrillo Y., Campos F., Reyes J.L., Garciarrubio A.,
RA Covarrubias A.A.;
RT "Functional analysis of the group 4 late embryogenesis abundant proteins
RT reveals their relevance in the adaptive response during water deficit in
RT Arabidopsis.";
RL Plant Physiol. 154:373-390(2010).
RN [8]
RP FUNCTION.
RX PubMed=27006402; DOI=10.1074/jbc.m116.720318;
RA Cuevas-Velazquez C.L., Saab-Rincon G., Reyes J.L., Covarrubias A.A.;
RT "The unstructured N-terminal region of Arabidopsis group 4 late
RT embryogenesis abundant (LEA) proteins is required for folding and for
RT chaperone-like activity under water deficit.";
RL J. Biol. Chem. 291:10893-10903(2016).
CC -!- FUNCTION: Involved dehydration tolerance. Involved in the adaptive
CC response of vascular plants to withstand water deficit
CC (PubMed:20668063). May possess chaperone-like activity under water
CC deficit (PubMed:27006402). {ECO:0000269|PubMed:20668063,
CC ECO:0000269|PubMed:27006402}.
CC -!- DEVELOPMENTAL STAGE: Expressed during embryo development and in dry
CC seed. Expression decreases significantly after seed germination.
CC {ECO:0000269|PubMed:20668063}.
CC -!- INDUCTION: By dehydration stress. {ECO:0000269|PubMed:20668063}.
CC -!- MISCELLANEOUS: Over-expression of LEA46 confers plant tolerance to
CC severe drought (PubMed:20668063). Under water deficit, the N-terminal
CC region is necessary and sufficient for conformational transition from
CC disordered to alpha-helix folding. This conformational transition is
CC required for chaperone-like activity under water limitation
CC (PubMed:27006402). {ECO:0000269|PubMed:20668063,
CC ECO:0000269|PubMed:27006402}.
CC -!- SIMILARITY: Belongs to the LEA type 1 family. {ECO:0000305}.
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DR EMBL; X91915; CAA63008.1; -; mRNA.
DR EMBL; AP002032; BAB09810.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91062.1; -; Genomic_DNA.
DR EMBL; AY050936; AAK93613.1; -; mRNA.
DR EMBL; AY091171; AAM14110.1; -; mRNA.
DR EMBL; Z27256; CAA81768.1; -; mRNA.
DR RefSeq; NP_196294.1; NM_120759.3.
DR AlphaFoldDB; Q9FG31; -.
DR SMR; Q9FG31; -.
DR STRING; 3702.AT5G06760.1; -.
DR PaxDb; Q9FG31; -.
DR PRIDE; Q9FG31; -.
DR ProMEX; Q9FG31; -.
DR ProteomicsDB; 230210; -.
DR EnsemblPlants; AT5G06760.1; AT5G06760.1; AT5G06760.
DR GeneID; 830565; -.
DR Gramene; AT5G06760.1; AT5G06760.1; AT5G06760.
DR KEGG; ath:AT5G06760; -.
DR Araport; AT5G06760; -.
DR TAIR; locus:2170239; AT5G06760.
DR eggNOG; ENOG502S22N; Eukaryota.
DR HOGENOM; CLU_114722_0_0_1; -.
DR InParanoid; Q9FG31; -.
DR OMA; PTGAHQM; -.
DR OrthoDB; 1573401at2759; -.
DR PhylomeDB; Q9FG31; -.
DR PRO; PR:Q9FG31; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FG31; baseline and differential.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005034; F:osmosensor activity; IDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IEA:InterPro.
DR GO; GO:0009409; P:response to cold; IDA:TAIR.
DR GO; GO:0006970; P:response to osmotic stress; IMP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR GO; GO:0048316; P:seed development; IMP:TAIR.
DR DisProt; DP02066; -.
DR InterPro; IPR005513; LEA_1.
DR PANTHER; PTHR33493; PTHR33493; 1.
DR Pfam; PF03760; LEA_1; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Stress response.
FT CHAIN 1..158
FT /note="Late embryogenesis abundant protein 46"
FT /id="PRO_0000438098"
FT REGION 1..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..67
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..142
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 58
FT /note="Q -> H (in Ref. 1; CAA63008)"
FT /evidence="ECO:0000305"
FT CONFLICT 64..65
FT /note="QH -> HT (in Ref. 5; CAA81768)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="A -> G (in Ref. 1; CAA63008)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 158 AA; 16179 MW; E0F088B82599F205 CRC64;
MQSMKETASN IAASAKSGMD KTKATLEEKA EKMKTRDPVQ KQMATQVKED KINQAEMQKR
ETRQHNAAMK EAAGAGTGLG LGTATHSTTG QVGHGTGTHQ MSALPGHGTG QLTDRVVEGT
AVTDPIGRNT GTGRTTAHNT HVGGGGATGY GTGGGYTG
