LEA7_ARATH
ID LEA7_ARATH Reviewed; 169 AA.
AC Q96270; Q42166; Q42240;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Late embryogenesis abundant protein 7 {ECO:0000303|PubMed:18318901};
GN Name=LEA7 {ECO:0000303|PubMed:18318901};
GN OrderedLocusNames=At1g52690 {ECO:0000312|Araport:AT1G52690};
GN ORFNames=F6D8.9 {ECO:0000312|EMBL:AAD55596.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Dry seed;
RA Raynal M.;
RT "A.thaliana mRNA for LEA76 type1 protein.";
RL Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION BY ABSCISIC ACID.
RX PubMed=16876791; DOI=10.1016/j.febslet.2006.07.047;
RA Robert N., Merlot S., N'guyen V., Boisson-Dernier A., Schroeder J.I.;
RT "A hypermorphic mutation in the protein phosphatase 2C HAB1 strongly
RT affects ABA signaling in Arabidopsis.";
RL FEBS Lett. 580:4691-4696(2006).
RN [7]
RP INDUCTION BY DROUGHT STRESS.
RX PubMed=16617101; DOI=10.1105/tpc.105.035881;
RA Sakuma Y., Maruyama K., Osakabe Y., Qin F., Seki M., Shinozaki K.,
RA Yamaguchi-Shinozaki K.;
RT "Functional analysis of an Arabidopsis transcription factor, DREB2A,
RT involved in drought-responsive gene expression.";
RL Plant Cell 18:1292-1309(2006).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18318901; DOI=10.1186/1471-2164-9-118;
RA Hundertmark M., Hincha D.K.;
RT "LEA (late embryogenesis abundant) proteins and their encoding genes in
RT Arabidopsis thaliana.";
RL BMC Genomics 9:118-118(2008).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=19325888; DOI=10.1371/journal.pgen.1000440;
RA Cheng H., Song S., Xiao L., Soo H.M., Cheng Z., Xie D., Peng J.;
RT "Gibberellin acts through jasmonate to control the expression of MYB21,
RT MYB24, and MYB57 to promote stamen filament growth in Arabidopsis.";
RL PLoS Genet. 5:E1000440-E1000440(2009).
RN [10]
RP SUBCELLULAR LOCATION.
RX PubMed=25005920; DOI=10.1105/tpc.114.127316;
RA Candat A., Paszkiewicz G., Neveu M., Gautier R., Logan D.C.,
RA Avelange-Macherel M.-H., Macherel D.;
RT "The ubiquitous distribution of late embryogenesis abundant proteins across
RT cell compartments in Arabidopsis offers tailored protection against abiotic
RT stress.";
RL Plant Cell 26:3148-3166(2014).
RN [11]
RP FUNCTION.
RX PubMed=25988244; DOI=10.1016/j.bbapap.2015.05.002;
RA Popova A.V., Rausch S., Hundertmark M., Gibon Y., Hincha D.K.;
RT "The intrinsically disordered protein LEA7 from Arabidopsis thaliana
RT protects the isolated enzyme lactate dehydrogenase and enzymes in a soluble
RT leaf proteome during freezing and drying.";
RL Biochim. Biophys. Acta 1854:1517-1525(2015).
CC -!- FUNCTION: Involved in dehydration and freezing tolerance. Protects and
CC stabilizes the enzyme activities of ADP-glucose-pyrophosphorylase
CC (AGPase) and glucose-6-phosphate dehydrogenase (G6PDH) during drought
CC stress and freezing. Prevents aggregation of leaf soluble proteins
CC during drought stress. Does not stabilize liposomes during drying and
CC rehydration. {ECO:0000269|PubMed:25988244}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:25005920}.
CC -!- TISSUE SPECIFICITY: Expressed in sepals, petals, pistils and stamens.
CC {ECO:0000269|PubMed:19325888}.
CC -!- INDUCTION: Induced by abscisic acid (ABA) (PubMed:16876791). Induced by
CC drought stress (PubMed:16617101). {ECO:0000269|PubMed:16617101,
CC ECO:0000269|PubMed:16876791}.
CC -!- SIMILARITY: Belongs to the LEA type 4 family. {ECO:0000305}.
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DR EMBL; X91919; CAA63012.1; -; mRNA.
DR EMBL; AC008016; AAD55596.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32838.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32839.1; -; Genomic_DNA.
DR EMBL; AF385732; AAK60322.1; -; mRNA.
DR EMBL; AY093968; AAM16229.1; -; mRNA.
DR EMBL; BT000737; AAN31879.1; -; mRNA.
DR EMBL; BT000739; AAN31880.1; -; mRNA.
DR EMBL; AY087466; AAM65011.1; -; mRNA.
DR PIR; H96567; H96567.
DR RefSeq; NP_175678.1; NM_104147.2.
DR RefSeq; NP_974009.1; NM_202280.2.
DR AlphaFoldDB; Q96270; -.
DR SMR; Q96270; -.
DR STRING; 3702.AT1G52690.1; -.
DR PaxDb; Q96270; -.
DR PRIDE; Q96270; -.
DR ProteomicsDB; 230335; -.
DR DNASU; 841701; -.
DR EnsemblPlants; AT1G52690.1; AT1G52690.1; AT1G52690.
DR EnsemblPlants; AT1G52690.2; AT1G52690.2; AT1G52690.
DR GeneID; 841701; -.
DR Gramene; AT1G52690.1; AT1G52690.1; AT1G52690.
DR Gramene; AT1G52690.2; AT1G52690.2; AT1G52690.
DR KEGG; ath:AT1G52690; -.
DR Araport; AT1G52690; -.
DR TAIR; locus:2035109; AT1G52690.
DR eggNOG; KOG4744; Eukaryota.
DR HOGENOM; CLU_100093_1_0_1; -.
DR InParanoid; Q96270; -.
DR OMA; ASHQEQS; -.
DR OrthoDB; 1531155at2759; -.
DR PhylomeDB; Q96270; -.
DR PRO; PR:Q96270; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q96270; baseline and differential.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0050821; P:protein stabilization; IDA:UniProtKB.
DR GO; GO:0050826; P:response to freezing; IDA:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IDA:UniProtKB.
DR DisProt; DP01858; -.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Stress response.
FT CHAIN 1..169
FT /note="Late embryogenesis abundant protein 7"
FT /id="PRO_0000438409"
FT REGION 1..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 139..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..110
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..169
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 169 AA; 18101 MW; 9E7BD0FC1280B373 CRC64;
MASHQEQSYK AGETRGKAQE KTGEAMGTMG DKTQAAKDKT QETAQSAQQK AHETAQSAKD
KTSQAAQTTQ ERAQESKDKT GSYMSETGEA IKNKAHDAAE YTKETAEAGK EKTSGILGQT
GEQVKQMAMG ATDAVKHTLG LRTDEGNKEH VSSAPSTTTT TTTRETQRK
