ID   LEAM_RAMVA              Reviewed;         293 AA.
AC   A0A0E4AVP3;
DT   07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT   24-JUN-2015, sequence version 1.
DT   25-MAY-2022, entry version 14.
DE   RecName: Full=Group 3 late-embryogenesis abundant protein, mitochondrial {ECO:0000303|PubMed:25675104};
DE            Short=LEAM {ECO:0000303|PubMed:25675104};
DE   Flags: Precursor;
GN   Name=RvLEAM {ECO:0000303|PubMed:25675104}; ORFNames=RvY_15023;
OS   Ramazzottius varieornatus (Water bear) (Tardigrade).
OC   Eukaryota; Metazoa; Ecdysozoa; Tardigrada; Eutardigrada; Parachela;
OC   Hypsibioidea; Ramazzottiidae; Ramazzottius.
OX   NCBI_TaxID=947166;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND DOMAIN.
RC   STRAIN=YOKOZUNA-1;
RX   PubMed=25675104; DOI=10.1371/journal.pone.0118272;
RA   Tanaka S., Tanaka J., Miwa Y., Horikawa D.D., Katayama T., Arakawa K.,
RA   Toyoda A., Kubo T., Kunieda T.;
RT   "Novel mitochondria-targeted heat-soluble proteins identified in the
RT   anhydrobiotic Tardigrade improve osmotic tolerance of human cells.";
RL   PLoS ONE 10:E0118272-E0118272(2015).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YOKOZUNA-1;
RX   PubMed=27649274; DOI=10.1038/ncomms12808;
RA   Hashimoto T., Horikawa D.D., Saito Y., Kuwahara H., Kozuka-Hata H.,
RA   Shin-I T., Minakuchi Y., Ohishi K., Motoyama A., Aizu T., Enomoto A.,
RA   Kondo K., Tanaka S., Hara Y., Koshikawa S., Sagara H., Miura T.,
RA   Yokobori S., Miyagawa K., Suzuki Y., Kubo T., Oyama M., Kohara Y.,
RA   Fujiyama A., Arakawa K., Katayama T., Toyoda A., Kunieda T.;
RT   "Extremotolerant tardigrade genome and improved radiotolerance of human
RT   cultured cells by tardigrade-unique protein.";
RL   Nat. Commun. 7:12808-12808(2016).
CC   -!- FUNCTION: Mitochondrial heat soluble protein acting as a molecular
CC       shield in water-deficient condition. {ECO:0000269|PubMed:25675104}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:25675104}.
CC   -!- DOMAIN: Contiguous repeats of the 11-mer LEA motif is characteristic of
CC       group 3 LEA proteins and form an amphipathic helical structure under
CC       water-deficient conditions (PubMed:25675104).
CC       {ECO:0000305|PubMed:25675104}.
CC   -!- MISCELLANEOUS: Improves osmotic tolerance of human cells
CC       (PubMed:25675104). {ECO:0000269|PubMed:25675104}.
CC   -!- SIMILARITY: Belongs to the LEA type 4 family. {ECO:0000305}.
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DR   EMBL; LC002821; BAQ94586.1; -; mRNA.
DR   EMBL; BDGG01000011; GAV04799.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0E4AVP3; -.
DR   Proteomes; UP000186922; Unassembled WGS sequence.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Mitochondrion; Reference proteome; Repeat; Stress response;
KW   Transit peptide.
FT   TRANSIT         1..31
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..293
FT                   /note="Group 3 late-embryogenesis abundant protein,
FT                   mitochondrial"
FT                   /id="PRO_0000440201"
FT   REPEAT          58..68
FT                   /note="LEA 11-mer repeat 1"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REPEAT          83..93
FT                   /note="LEA 11-mer repeat 2"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REPEAT          123..133
FT                   /note="LEA 11-mer repeat 3"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REPEAT          134..144
FT                   /note="LEA 11-mer repeat 4"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REPEAT          145..155
FT                   /note="LEA 11-mer repeat 5"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REPEAT          160..170
FT                   /note="LEA 11-mer repeat 6"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REPEAT          171..181
FT                   /note="LEA 11-mer repeat 7"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REPEAT          199..209
FT                   /note="LEA 11-mer repeat 8"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REPEAT          210..220
FT                   /note="LEA 11-mer repeat 9"
FT                   /evidence="ECO:0000305|PubMed:25675104"
FT   REGION          27..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          217..293
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        226..274
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   293 AA;  30485 MW;  D33799E042CAC912 CRC64;
     MFLARNAGRA GYRGVVAYQQ AASFSVSSAK AAGSRSSGGS DAGDYAREAA EHAKAGLKDL
     KNEASWKAKG VANQAAGAFE RAKDTVKEGV HDMKRSGSRV FEQGQEEVEA GAQHAKAGYQ
     SAKNAAQDTA ATLKDKAGSA WNQAKHVVED KGEDVVEAVK DTASKVWGKA KHVAEDVKEN
     AQSPGGIADK ASDVWSAAKD KAADVLSGAK HTAENLAHKA QAAIHDATAS SGSQSQSQSQ
     SQYRQGQQQG RQDQQQSKSQ WGQTSPQSPD GFRPQAGQGP QGGKGPGQAG GRR