ID   LEB1_GALME              Reviewed;          42 AA.
AC   P85211;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Lebocin-like anionic peptide 1;
OS   Galleria mellonella (Greater wax moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea;
OC   Pyralidae; Galleriinae; Galleria.
OX   NCBI_TaxID=7137;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP   INDUCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Larval hemolymph {ECO:0000269|PubMed:17194500};
RX   PubMed=17194500; DOI=10.1016/j.peptides.2006.11.010;
RA   Cytrynska M., Mak P., Zdybicka-Barabas A., Suder P., Jakubowicz T.;
RT   "Purification and characterization of eight peptides from Galleria
RT   mellonella immune hemolymph.";
RL   Peptides 28:533-546(2007).
CC   -!- FUNCTION: Antimicrobial protein. Has antibacterial activity against the
CC       Gram-positive bacteria M.luteus (MIC=22.7 uM) and L.monocytogenes
CC       (MIC=90.9 uM). Lacks antibacterial activity against the Gram-positive
CC       bacteria B.circulans, S.aureus, and S.lutea, and the Gram-negative
CC       bacteria E.coli D31, E.coli ATCC 25922, and S.typhimurium. Has
CC       antifungal activity against A.niger (MIC=90.9 uM) and T.harzianum
CC       (MIC=90.9 uM), but lacks antifungal activity against S.cerevisiae,
CC       P.pastoris, Z.marxianus, C.albicans, C.fructus, and F.oxysporum.
CC       {ECO:0000269|PubMed:17194500}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17194500}.
CC   -!- TISSUE SPECIFICITY: Hemolymph. {ECO:0000269|PubMed:17194500}.
CC   -!- INDUCTION: By bacterial infection. {ECO:0000269|PubMed:17194500}.
CC   -!- MASS SPECTROMETRY: Mass=4820.1; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:17194500};
CC   -!- SIMILARITY: Belongs to the lebocin family. {ECO:0000255}.
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DR   AlphaFoldDB; P85211; -.
DR   Proteomes; UP000504614; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide; Immunity;
KW   Innate immunity; Reference proteome; Secreted.
FT   PEPTIDE         1..42
FT                   /note="Lebocin-like anionic peptide 1"
FT                   /evidence="ECO:0000269|PubMed:17194500"
FT                   /id="PRO_0000298771"
SQ   SEQUENCE   42 AA;  4819 MW;  585F885D72EDCE64 CRC64;
     EADEPLWLYK GDNIERAPTT ADHPILPSII DDVKLDPNRR YA