LEC2_ARATH
ID LEC2_ARATH Reviewed; 363 AA.
AC Q1PFR7; A0ME96; Q93VR5; Q9FZA3;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=B3 domain-containing transcription factor LEC2;
DE AltName: Full=Protein LEAFY COTYLEDON 2;
GN Name=LEC2; OrderedLocusNames=At1g28300; ORFNames=F3H9.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=11573014; DOI=10.1073/pnas.201413498;
RA Stone S.L., Kwong L.W., Yee K.M., Pelletier J., Lepiniec L., Fischer R.L.,
RA Goldberg R.B., Harada J.J.;
RT "LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces
RT embryo development.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11806-11811(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=15208423; DOI=10.1104/pp.104.042176;
RA Gong W., Shen Y.-P., Ma L.-G., Pan Y., Du Y.-L., Wang D.-H., Yang J.-Y.,
RA Hu L.-D., Liu X.-F., Dong C.-X., Ma L., Chen Y.-H., Yang X.-Y., Gao Y.,
RA Zhu D., Tan X., Mu J.-Y., Zhang D.-B., Liu Y.-L., Dinesh-Kumar S.P., Li Y.,
RA Wang X.-P., Gu H.-Y., Qu L.-J., Bai S.-N., Lu Y.-T., Li J.-Y., Zhao J.-D.,
RA Zuo J., Huang H., Deng X.-W., Zhu Y.-X.;
RT "Genome-wide ORFeome cloning and analysis of Arabidopsis transcription
RT factor genes.";
RL Plant Physiol. 135:773-782(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=12244265; DOI=10.2307/3869884;
RA Meinke D.W., Franzmann L.H., Nickle T.C., Yeung E.C.;
RT "Leafy cotyledon mutants of Arabidopsis.";
RL Plant Cell 6:1049-1064(1994).
RN [7]
RP FUNCTION.
RX PubMed=16492731; DOI=10.1073/pnas.0511331103;
RA Braybrook S.A., Stone S.L., Park S., Bui A.Q., Le B.H., Fischer R.L.,
RA Goldberg R.B., Harada J.J.;
RT "Genes directly regulated by LEAFY COTYLEDON2 provide insight into the
RT control of embryo maturation and somatic embryogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:3468-3473(2006).
RN [8]
RP FUNCTION.
RX PubMed=18287041; DOI=10.1073/pnas.0712364105;
RA Stone S.L., Braybrook S.A., Paula S.L., Kwong L.W., Meuser J.,
RA Pelletier J., Hsieh T.-F., Fischer R.L., Goldberg R.B., Harada J.J.;
RT "Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity:
RT implications for somatic embryogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:3151-3156(2008).
RN [9]
RP GENE FAMILY.
RX PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA Swaminathan K., Peterson K., Jack T.;
RT "The plant B3 superfamily.";
RL Trends Plant Sci. 13:647-655(2008).
CC -!- FUNCTION: Transcription regulator that plays a central role in embryo
CC development. Required for the maintenance of suspensor morphology,
CC specification of cotyledon identity, progression through the maturation
CC phase and suppression of premature germination. Ectopic expression is
CC sufficient to promote somatic embryogenesis.
CC {ECO:0000269|PubMed:11573014, ECO:0000269|PubMed:16492731,
CC ECO:0000269|PubMed:18287041}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed during embryo development.
CC {ECO:0000269|PubMed:11573014}.
CC -!- DISRUPTION PHENOTYPE: Pigmented seeds. Distorted seedlings with
CC elongated hypocotyl and curled cotyledons. Presence of trichomes and
CC accumulation of anthocyanins on cotyledons. Unusual pattern of storage
CC product accumulation in seedlings. {ECO:0000269|PubMed:12244265}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF98425.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=ABK28418.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF400123; AAL12004.1; -; mRNA.
DR EMBL; AF400124; AAL12005.1; -; Genomic_DNA.
DR EMBL; AC021044; AAF98425.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30945.1; -; Genomic_DNA.
DR EMBL; AJ630496; CAG25869.1; -; mRNA.
DR EMBL; AY568668; AAS79558.1; -; mRNA.
DR EMBL; DQ446296; ABE65660.1; -; mRNA.
DR EMBL; DQ652865; ABK28418.1; ALT_SEQ; mRNA.
DR PIR; C86409; C86409.
DR RefSeq; NP_564304.1; NM_102595.2.
DR PDB; 6J9C; X-ray; 3.10 A; A/D=160-273.
DR PDBsum; 6J9C; -.
DR AlphaFoldDB; Q1PFR7; -.
DR SMR; Q1PFR7; -.
DR BioGRID; 24959; 1.
DR STRING; 3702.AT1G28300.1; -.
DR PaxDb; Q1PFR7; -.
DR PRIDE; Q1PFR7; -.
DR EnsemblPlants; AT1G28300.1; AT1G28300.1; AT1G28300.
DR GeneID; 839724; -.
DR Gramene; AT1G28300.1; AT1G28300.1; AT1G28300.
DR KEGG; ath:AT1G28300; -.
DR Araport; AT1G28300; -.
DR TAIR; locus:2032170; AT1G28300.
DR eggNOG; ENOG502S2IE; Eukaryota.
DR HOGENOM; CLU_768029_0_0_1; -.
DR InParanoid; Q1PFR7; -.
DR OMA; MTKMARI; -.
DR OrthoDB; 911852at2759; -.
DR PhylomeDB; Q1PFR7; -.
DR PRO; PR:Q1PFR7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q1PFR7; baseline and differential.
DR Genevisible; Q1PFR7; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0010601; P:positive regulation of auxin biosynthetic process; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0010431; P:seed maturation; IMP:TAIR.
DR GO; GO:0010344; P:seed oilbody biogenesis; IMP:TAIR.
DR GO; GO:0010262; P:somatic embryogenesis; IMP:TAIR.
DR CDD; cd10017; B3_DNA; 1.
DR Gene3D; 2.40.330.10; -; 1.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR InterPro; IPR044800; LEC2-like.
DR PANTHER; PTHR31140; PTHR31140; 1.
DR Pfam; PF02362; B3; 1.
DR SMART; SM01019; B3; 1.
DR SUPFAM; SSF101936; SSF101936; 1.
DR PROSITE; PS50863; B3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..363
FT /note="B3 domain-containing transcription factor LEC2"
FT /id="PRO_0000375090"
FT DNA_BIND 171..272
FT /note="TF-B3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 339..341
FT /note="SMP -> MT (in Ref. 1; AAL12004/AAL12005 and 4;
FT AAS79558/CAG25869)"
FT /evidence="ECO:0000305"
FT STRAND 167..174
FT /evidence="ECO:0007829|PDB:6J9C"
FT TURN 177..180
FT /evidence="ECO:0007829|PDB:6J9C"
FT STRAND 185..188
FT /evidence="ECO:0007829|PDB:6J9C"
FT HELIX 190..196
FT /evidence="ECO:0007829|PDB:6J9C"
FT STRAND 206..211
FT /evidence="ECO:0007829|PDB:6J9C"
FT STRAND 218..228
FT /evidence="ECO:0007829|PDB:6J9C"
FT STRAND 231..237
FT /evidence="ECO:0007829|PDB:6J9C"
FT HELIX 240..246
FT /evidence="ECO:0007829|PDB:6J9C"
FT STRAND 253..262
FT /evidence="ECO:0007829|PDB:6J9C"
FT STRAND 269..271
FT /evidence="ECO:0007829|PDB:6J9C"
SQ SEQUENCE 363 AA; 41708 MW; 5CF6D8ABE53CD45A CRC64;
MDNFLPFPSS NANSVQELSM DPNNNRSHFT TVPTYDHHQA QPHHFLPPFS YPVEQMAAVM
NPQPVYLSEC YPQIPVTQTG SEFGSLVGNP CLWQERGGFL DPRMTKMARI NRKNAMMRSR
NNSSPNSSPS ELVDSKRQLM MLNLKNNVQI SDKKDSYQQS TFDNKKLRVL CEKELKNSDV
GSLGRIVLPK RDAEANLPKL SDKEGIVVQM RDVFSMQSWS FKYKFWSNNK SRMYVLENTG
EFVKQNGAEI GDFLTIYEDE SKNLYFAMNG NSGKQNEGRE NESRERNHYE EAMLDYIPRD
EEEASIAMLI GNLNDHYPIP NDLMDLTTDL QHHQATSSSM PPEDHAYVGS SDDQVSFNDF
EWW
