ID   LEC3B_HALCE             Reviewed;         106 AA.
AC   C0HK21;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   07-SEP-2016, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Halilectin 3, beta chain {ECO:0000305|PubMed:24144578};
DE            Short=H-3 {ECO:0000303|PubMed:24144578};
OS   Haliclona caerulea (Blue Caribbean sponge) (Sigmadocia caerulea).
OC   Eukaryota; Metazoa; Porifera; Demospongiae; Heteroscleromorpha;
OC   Haplosclerida; Chalinidae; Haliclona.
OX   NCBI_TaxID=1131259 {ECO:0000303|PubMed:24144578};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT,
RP   GLYCOSYLATION, MASS SPECTROMETRY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=24144578; DOI=10.1016/j.biocel.2013.10.005;
RA   Carneiro R.F., de Melo A.A., de Almeida A.S., Moura Rda M., Chaves R.P.,
RA   de Sousa B.L., do Nascimento K.S., Sampaio S.S., Lima J.P., Cavada B.S.,
RA   Nagano C.S., Sampaio A.H.;
RT   "H-3, a new lectin from the marine sponge Haliclona caerulea: purification
RT   and mass spectrometric characterization.";
RL   Int. J. Biochem. Cell Biol. 45:2864-2873(2013).
CC   -!- FUNCTION: Lectin with affinity for N-acetyl-galactosamine, carragenan
CC       and glycoprotein porcine stomach mucin (PSM). Has metal-independent
CC       hemagglutinating activity towards erythrocytes from rabbit and human.
CC       Hemagglutinating activity is not inhibited by D-galactose, D-glucose,
CC       D-mannose, D-fucose, methyl-alpha-D-galactopyranoside, methyl-alpha-D-
CC       glucopyranoside, N-acetyl-glucosamine, N-acetyl-mannosamine, D-
CC       fructose, alpha-D-lactose, beta-D-lactose, D-lactulose, D-sucrose,
CC       fucoidan or glycoproteins thyroglobulin and ovalmucoid.
CC       {ECO:0000269|PubMed:24144578}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 6-7 for hemagglutinating activity. Activity drops
CC         rapidly at lower or higher pH. {ECO:0000269|PubMed:24144578};
CC       Temperature dependence:
CC         Thermostable. Retains hemagglutinating activity after incubation at
CC         60 degrees Celsius for 1 hour. At higher temperatures activity drops
CC         drastically. {ECO:0000269|PubMed:24144578};
CC   -!- SUBUNIT: Probable heterotrimer consisting of an alpha chain and two
CC       beta chains. The alpha chain can probably have different glycosylation
CC       states. {ECO:0000269|PubMed:24144578}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:24144578}.
CC   -!- MASS SPECTROMETRY: Mass=11828; Mass_error=2; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:24144578};
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DR   AlphaFoldDB; C0HK21; -.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Lectin.
FT   CHAIN           1..106
FT                   /note="Halilectin 3, beta chain"
FT                   /evidence="ECO:0000269|PubMed:24144578"
FT                   /id="PRO_0000437087"
FT   CARBOHYD        65
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   106 AA;  11624 MW;  D4C532EE5E9F4568 CRC64;
     AATCKETPPT WSGHLFEIST DNPRRADISY SREEKKIDTT DYKGVPLRTT LDDYATGTKY
     CRKSNLTGTI PTFGVPDKLP SPAGPHYLGG KLPSTGVLVL DFYGEK