LEC5_MEDTR
ID LEC5_MEDTR Reviewed; 282 AA.
AC A0A396HQY2; A9YWS2; Q2PP78;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-DEC-2018, sequence version 1.
DT 25-MAY-2022, entry version 14.
DE RecName: Full=Lectin 5 {ECO:0000303|PubMed:16134889};
DE Short=MtLec5 {ECO:0000303|PubMed:16134889};
DE AltName: Full=Agglutinin LEC5 {ECO:0000305};
GN Name=LEC5 {ECO:0000303|PubMed:16134889};
GN OrderedLocusNames=MTR_5g031030 {ECO:0000312|EMBL:AES95920.1};
GN ORFNames=MtrunA17_Chr5g0411721 {ECO:0000312|EMBL:RHN54883.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=16273388; DOI=10.1007/s00438-005-0057-9;
RA Kevei Z., Seres A., Kereszt A., Kalo P., Kiss P., Toth G., Endre G.,
RA Kiss G.B.;
RT "Significant microsynteny with new evolutionary highlights is detected
RT between Arabidopsis and legume model plants despite the lack of
RT macrosynteny.";
RL Mol. Genet. Genomics 274:644-657(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 9-282, INDUCTION BY ARBUSCULAR MYCORRHIZAL
RP FUNGI, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Jemalong A17;
RX PubMed=16134889; DOI=10.1094/mpmi-18-0771;
RA Frenzel A., Manthey K., Perlick A.M., Meyer F., Puehler A., Kuester H.,
RA Krajinski F.;
RT "Combined transcriptome profiling reveals a novel family of arbuscular
RT mycorrhizal-specific Medicago truncatula lectin genes.";
RL Mol. Plant Microbe Interact. 18:771-782(2005).
RN [6]
RP INDUCTION BY RAM1 AND GLOMUS VERSIFORME.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
CC -!- FUNCTION: May be involved in arbuscular mycorrhizal (AM) symbiosis with
CC AM fungi. {ECO:0000305|PubMed:16134889}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: Expressed in cortical cells containing arbuscules
CC of mycorrhizal roots upon arbuscular mycorrhizal (AM) symbiosis with AM
CC fungi (e.g. Glomus intraradices). {ECO:0000269|PubMed:16134889}.
CC -!- INDUCTION: Accumulates in roots, in a RAM1-dependent manner, during
CC colonization by arbuscular mycorrhizal (AM) fungi (e.g. Glomus
CC versiforme and G.intraradices). {ECO:0000269|PubMed:16134889,
CC ECO:0000269|PubMed:26511916}.
CC -!- SIMILARITY: Belongs to the leguminous lectin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABY48144.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AES95920.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; EU306659; ABY48144.1; ALT_INIT; Genomic_DNA.
DR EMBL; PSQE01000005; RHN54883.1; -; Genomic_DNA.
DR EMBL; CM001221; AES95920.1; ALT_INIT; Genomic_DNA.
DR EMBL; DQ314207; ABC47811.1; -; mRNA.
DR RefSeq; XP_003612962.1; XM_003612914.1.
DR AlphaFoldDB; A0A396HQY2; -.
DR SMR; A0A396HQY2; -.
DR STRING; 3880.AES95920; -.
DR EnsemblPlants; AES95920; AES95920; MTR_5g031030.
DR GeneID; 11416509; -.
DR Gramene; AES95920; AES95920; MTR_5g031030.
DR KEGG; mtr:MTR_5g031030; -.
DR eggNOG; ENOG502QTX3; Eukaryota.
DR HOGENOM; CLU_000288_62_2_1; -.
DR OrthoDB; 1114765at2759; -.
DR Proteomes; UP000002051; Chromosome 5.
DR Proteomes; UP000265566; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR CDD; cd06899; lectin_legume_LecRK_Arcelin_ConA; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR016363; L-lectin.
DR InterPro; IPR000985; Lectin_LegA_CS.
DR InterPro; IPR019825; Lectin_legB_Mn/Ca_BS.
DR InterPro; IPR001220; Legume_lectin_dom.
DR Pfam; PF00139; Lectin_legB; 1.
DR PIRSF; PIRSF002690; L-type_lectin_plant; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS00308; LECTIN_LEGUME_ALPHA; 1.
DR PROSITE; PS00307; LECTIN_LEGUME_BETA; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Lectin; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..282
FT /note="Lectin 5"
FT /id="PRO_0000450044"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..282
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT CARBOHYD 161
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CONFLICT 92..94
FT /note="AFF -> SFL (in Ref. 5; ABC47811)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 282 AA; 30778 MW; 5069079ACD7B167F CRC64;
MHHTQHNTMA NSIPKLLATQ NPFSVSLSIF FFLLLLINNV KSDSLSFNFP KFDTDALNIV
IDGDAKTTGG VLQLTKKDQF GNPSPHSVGF SAFFGAIQLS DKQSGKVADF TTEFSFVVNP
KGSQLHGDGF AFYIASLDYD FPEKSSDGGF LGLFDKETAF NTSKNSIVAV EFDSFANEWD
PNSPHIGIDI NTIESSISVP WPIDRQPQGT IGKARISYNT ASKDLSVFVT YPNSPAKVDV
IVSYPIDFAS VLSEWVYVGF SGATGQVAET HDILSWSFVS NL
