ID   LECA_IRIHO              Reviewed;          28 AA.
AC   P36230;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=N-acetyl-D-galactosamine-binding lectin subunit A;
DE            EC=3.2.2.22;
DE   AltName: Full=A-disaccharide-binding lectin subunit A;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Fragment;
OS   Iris hollandica (Dutch iris) (Iris tingitana x Iris xiphium).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Iridaceae;
OC   Iridoideae; Irideae; Iris.
OX   NCBI_TaxID=35876;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. Golden Harvest, and cv. Prof. Blaauw; TISSUE=Bulb;
RX   PubMed=8125993; DOI=10.1016/s0021-9258(17)37339-8;
RA   Mo H., van Damme E.J.M., Peumans W.J., Goldstein I.J.;
RT   "Isolation and characterization of an N-acetyl-D-galactosamine-binding
RT   lectin from Dutch Iris bulbs which recognizes the blood group A
RT   disaccharide (GalNAc alpha 1-3Gal).";
RL   J. Biol. Chem. 269:7666-7673(1994).
CC   -!- FUNCTION: Gal / GalNAc-specific lectin. Agglutinates both native and
CC       trypsin-treated rabbit erythrocytes but not human erythrocytes
CC       irrespective of blood group type.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SUBUNIT: Disulfide-linked heterodimer of A and B chains.
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family.
CC       {ECO:0000305}.
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DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   SUPFAM; SSF56371; SSF56371; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Hydrolase; Lectin;
KW   Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..>28
FT                   /note="N-acetyl-D-galactosamine-binding lectin subunit A"
FT                   /id="PRO_0000221422"
FT   NON_TER         28
SQ   SEQUENCE   28 AA;  3270 MW;  6D806E619B97E05D CRC64;
     YDKVEFHITG XTKDTYSAFI QSLRTHLS