LECA_MACPO
ID LECA_MACPO Reviewed; 133 AA.
AC P18674;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 2.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Agglutinin alpha chain;
DE AltName: Full=MPA;
OS Maclura pomifera (Osage orange) (Ioxylon pomiferum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Moraceae; Maclura.
OX NCBI_TaxID=3496;
RN [1]
RP PROTEIN SEQUENCE.
RC TISSUE=Seed;
RX PubMed=2037053; DOI=10.1016/0014-5793(91)80518-8;
RA Young N.M., Johnston R.A.Z., Watson D.C.;
RT "The amino acid sequences of jacalin and the Maclura pomifera agglutinin.";
RL FEBS Lett. 282:382-384(1991).
RN [2]
RP PROTEIN SEQUENCE OF 1-33.
RC TISSUE=Seed;
RX PubMed=2705782; DOI=10.1016/0003-9861(89)90542-0;
RA Young N.M., Johnston R.A.Z., Szabo A.G., Watson D.C.;
RT "Homology of the D-galactose-specific lectins from Artocarpus integrifolia
RT and Maclura pomifera and the role of an unusual small polypeptide
RT subunit.";
RL Arch. Biochem. Biophys. 270:596-603(1989).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX PubMed=9497359; DOI=10.1074/jbc.273.11.6312;
RA Lee X., Thompson A., Zhang Z., Ton-That H., Biesterfeldt J., Ogata C.,
RA Xu L., Johnston R.A., Young N.M.;
RT "Structure of the complex of Maclura pomifera agglutinin and the T-antigen
RT disaccharide, Galbeta1,3GalNAc.";
RL J. Biol. Chem. 273:6312-6318(1998).
CC -!- FUNCTION: D-galactose-specific lectin, binds the T-antigen structure
CC Gal-beta1,3-GalNAc.
CC -!- SUBUNIT: Formed of four alpha chains and four beta chains.
CC -!- SIMILARITY: Belongs to the jacalin lectin family. {ECO:0000255|PROSITE-
CC ProRule:PRU01088, ECO:0000305}.
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DR PIR; S03990; S03990.
DR PIR; S15825; S15825.
DR PDB; 1JOT; X-ray; 2.20 A; A=1-133.
DR PDB; 3LLY; X-ray; 2.25 A; A=1-133.
DR PDB; 3LLZ; X-ray; 1.55 A; A=1-133.
DR PDB; 3LM1; X-ray; 2.10 A; A/C/E/G/I/K/M/O=1-133.
DR PDBsum; 1JOT; -.
DR PDBsum; 3LLY; -.
DR PDBsum; 3LLZ; -.
DR PDBsum; 3LM1; -.
DR AlphaFoldDB; P18674; -.
DR SMR; P18674; -.
DR EvolutionaryTrace; P18674; -.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR CDD; cd09612; Jacalin; 1.
DR Gene3D; 2.100.10.30; -; 1.
DR InterPro; IPR001229; Jacalin-like_lectin_dom.
DR InterPro; IPR033734; Jacalin-like_lectin_dom_plant.
DR InterPro; IPR036404; Jacalin-like_lectin_dom_sf.
DR Pfam; PF01419; Jacalin; 1.
DR SMART; SM00915; Jacalin; 1.
DR SUPFAM; SSF51101; SSF51101; 1.
DR PROSITE; PS51752; JACALIN_LECTIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Lectin.
FT CHAIN 1..133
FT /note="Agglutinin alpha chain"
FT /id="PRO_0000072803"
FT DOMAIN 1..133
FT /note="Jacalin-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01088"
FT VARIANT 31
FT /note="T -> V (in minor forms)"
FT VARIANT 52
FT /note="K -> T (in minor forms)"
FT VARIANT 59
FT /note="E -> D (in minor forms)"
FT VARIANT 72
FT /note="V -> I (in minor forms)"
FT VARIANT 81
FT /note="I -> V (in minor forms)"
FT VARIANT 110
FT /note="N -> Q (in minor forms)"
FT VARIANT 112
FT /note="L -> G (in minor forms)"
FT CONFLICT 29
FT /note="R -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 32..33
FT /note="YD -> QN (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 2..5
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 10..19
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 21..23
FT /evidence="ECO:0007829|PDB:1JOT"
FT STRAND 25..34
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 37..40
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 52..57
FT /evidence="ECO:0007829|PDB:3LLZ"
FT TURN 60..62
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 65..75
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 78..90
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 92..97
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 101..107
FT /evidence="ECO:0007829|PDB:3LLZ"
FT STRAND 112..132
FT /evidence="ECO:0007829|PDB:3LLZ"
SQ SEQUENCE 133 AA; 14758 MW; 15C69FF94B6D09FD CRC64;
GVTFDDGAYT GIREINFEYN SETAIGGLRV TYDLNGMPFV AEDHKSFITG FKPVKISLEF
PSEYIVEVSG YVGKVEGYTV IRSLTFKTNK QTYGPYGVTN GTPFSLPIEN GLIVGFKGSI
GYWLDYFSIY LSL
