ID   LECB_STYJP              Reviewed;         270 AA.
AC   P93538;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Bark lectin;
DE   AltName: Full=LECSJABG;
DE   Flags: Precursor; Fragment;
OS   Styphnolobium japonicum (Japanese pagoda tree) (Sophora japonica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; Cladrastis clade;
OC   Styphnolobium.
OX   NCBI_TaxID=3897;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Bark;
RX   PubMed=9049272; DOI=10.1023/a:1005781103418;
RA   van Damme E.J., Barre A., Rouge P., Peumans W.J.;
RT   "Molecular cloning of the bark and seed lectins from the Japanese pagoda
RT   tree (Sophora japonica).";
RL   Plant Mol. Biol. 33:523-536(1997).
CC   -!- FUNCTION: GalNAc-specific lectin.
CC   -!- SIMILARITY: Belongs to the leguminous lectin family. {ECO:0000305}.
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DR   EMBL; U63014; AAB51458.1; -; mRNA.
DR   AlphaFoldDB; P93538; -.
DR   SMR; P93538; -.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd06899; lectin_legume_LecRK_Arcelin_ConA; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR016363; L-lectin.
DR   InterPro; IPR000985; Lectin_LegA_CS.
DR   InterPro; IPR019825; Lectin_legB_Mn/Ca_BS.
DR   InterPro; IPR001220; Legume_lectin_dom.
DR   Pfam; PF00139; Lectin_legB; 1.
DR   PIRSF; PIRSF002690; L-type_lectin_plant; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   PROSITE; PS00308; LECTIN_LEGUME_ALPHA; 1.
DR   PROSITE; PS00307; LECTIN_LEGUME_BETA; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Glycoprotein; Lectin; Manganese; Metal-binding; Signal.
FT   SIGNAL          <1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..270
FT                   /note="Bark lectin"
FT                   /id="PRO_0000017654"
FT   BINDING         141
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         143
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         143
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         145
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         147
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         150
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         150
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         155
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        60
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        76
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        127
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        201
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
SQ   SEQUENCE   270 AA;  29315 MW;  1FD655A2C4E550B3 CRC64;
     ISITFFLLLL NKVNSAEILS FSFPKFVSNQ EDLLLQGDAL VSSEGELQLT TVENGVPVWN
     STGRALYYAP VHIWDNSTGR VASFATSFSF VVKAPVASKS ADGIAFFLAP LNNQIHGAGG
     GLYGLFNSSS YSSSYQIVAV EFDTHTNAWD PNTRHIGIDV NSVKSTKTVT WGWENGEVAN
     VLITYQAATE MLTVSLTYPS NQTSYILSAA VDLKSILPEW VRVGFTATTG LTTQYVETND
     VLSWSFTSTL ETSDCGAEDN NVHLASYAFI