ID   LECG2_APLKU             Reviewed;          25 AA.
AC   B3EWI0;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Galactose-binding lectin-2 {ECO:0000303|PubMed:21953532};
DE            Short=AKL-2 {ECO:0000303|PubMed:21953532};
DE   Flags: Fragment;
OS   Aplysia kurodai (Kuroda's sea hare).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC   Aplysiidae; Aplysia.
OX   NCBI_TaxID=6501;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AND
RP   GLYCOSYLATION.
RC   TISSUE=Egg {ECO:0000269|PubMed:21953532};
RX   PubMed=21953532; DOI=10.1007/s10930-011-9356-7;
RA   Kawsar S.M., Matsumoto R., Fujii Y., Matsuoka H., Masuda N., Chihiro I.,
RA   Yasumitsu H., Kanaly R.A., Sugawara S., Hosono M., Nitta K., Ishizaki N.,
RA   Dogasaki C., Hamako J., Matsui T., Ozeki Y.;
RT   "Cytotoxicity and glycan-binding profile of a D-galactose-binding lectin
RT   from the eggs of a Japanese sea hare (Aplysia kurodai).";
RL   Protein J. 30:509-519(2011).
CC   -!- FUNCTION: D-galactose specific lectin. Binds in decreasing order of
CC       affinity: melibiose, N-acetyllactosamine, D-galacturonic acid, D-
CC       galactose, methyl-alpha-D-galactoside, D-galactose, methyl-alpha-D-
CC       galactopyranoside, methyl-beta-D-galactopyranoside and lactose. Binds
CC       also the glycoproteins globotriose, asialofetuin and mucin. Possesses
CC       glycan-dependent cytotoxic activity against Burkitt's lymphoma Raji
CC       cells and erythroleukemia K562 cells. Has calcium-independent
CC       hemagglutinating activity towards human erythrocytes.
CC       {ECO:0000269|PubMed:21953532}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Hemagglutinating activity is stable over the pH range 4.0-12.0 but
CC         decreases below pH 3. {ECO:0000269|PubMed:21953532};
CC       Temperature dependence:
CC         Hemagglutinating activity is stable when incubated over the range of
CC         20-70 degrees Celsius for 60 minutes but is almost lost after
CC         incubation for 60 minutes at 80 degrees Celsius.
CC         {ECO:0000269|PubMed:21953532};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:21953532}.
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:21953532}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; B3EWI0; -.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Hemagglutinin; Lectin.
FT   CHAIN           1..>25
FT                   /note="Galactose-binding lectin-2"
FT                   /id="PRO_0000417399"
FT   NON_TER         25
FT                   /evidence="ECO:0000303|PubMed:21953532"
SQ   SEQUENCE   25 AA;  2700 MW;  D30BA13E2FFA72DE CRC64;
     VLYQAVNVFS MDVIDVNSAA PKTCD