ID   LECH_HORVV              Reviewed;         146 AA.
AC   P82953; Q8W009;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 2.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Horcolin;
DE   AltName: Full=Agglutinin;
DE   AltName: Full=Mannose-specific lectin;
OS   Hordeum vulgare subsp. vulgare (Domesticated barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=112509;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-16; 32-52; 59-97 AND
RP   103-108, FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Apex; TISSUE=Coleoptile;
RX   PubMed=17245569; DOI=10.1007/s00425-006-0467-x;
RA   Grunwald I., Heinig I., Thole H.H., Neumann D., Kahmann U., Kloppstech K.,
RA   Gau A.E.;
RT   "Purification and characterisation of a jacalin-related, coleoptile
RT   specific lectin from Hordeum vulgare.";
RL   Planta 226:225-234(2007).
CC   -!- FUNCTION: Mannose-specific lectin. Has a weak agglutinating activity
CC       against rabbit erythrocytes. {ECO:0000269|PubMed:17245569}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000269|PubMed:17245569, ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in both light- and dark-grown
CC       coleoptiles, but not in leaves or roots. {ECO:0000269|PubMed:17245569}.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined MW of this protein is: 15.3
CC       kDa.
CC   -!- SIMILARITY: Belongs to the jacalin lectin family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01088, ECO:0000305}.
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DR   EMBL; AY033628; AAK54458.1; -; mRNA.
DR   AlphaFoldDB; P82953; -.
DR   SMR; P82953; -.
DR   PRIDE; P82953; -.
DR   eggNOG; ENOG502S4MA; Eukaryota.
DR   Proteomes; UP000011116; Unassembled WGS sequence.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005537; F:mannose binding; IEA:UniProtKB-KW.
DR   CDD; cd09612; Jacalin; 1.
DR   Gene3D; 2.100.10.30; -; 1.
DR   InterPro; IPR001229; Jacalin-like_lectin_dom.
DR   InterPro; IPR033734; Jacalin-like_lectin_dom_plant.
DR   InterPro; IPR036404; Jacalin-like_lectin_dom_sf.
DR   Pfam; PF01419; Jacalin; 1.
DR   SMART; SM00915; Jacalin; 1.
DR   SUPFAM; SSF51101; SSF51101; 1.
DR   PROSITE; PS51752; JACALIN_LECTIN; 1.
PE   1: Evidence at protein level;
KW   Apoplast; Direct protein sequencing; Lectin; Mannose-binding;
KW   Reference proteome; Secreted.
FT   CHAIN           1..146
FT                   /note="Horcolin"
FT                   /id="PRO_0000072805"
FT   DOMAIN          4..146
FT                   /note="Jacalin-type lectin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01088"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   146 AA;  15122 MW;  E5417043C6FCA0A8 CRC64;
     MSKPVKIGPW GGNGGSERDV QPKPIRMVSM TVSSGAIVDA IAFTYVGTDN VQHSSGIKWG
     GTGGTEDTIN LDATNYVTEI SGTVGKFGTD DIVTSLKIIT SKGVTRTYGS GTGIPFRVPV
     LDGGKIAGFF GRAGAFLDAI GFYITP