LECT2_ARATH
ID LECT2_ARATH Reviewed; 271 AA.
AC Q9LJR2; Q39205; Q8L715;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Lectin-like protein LEC;
DE Short=AtLEC;
DE Short=Ath.lec2;
DE Flags: Precursor;
GN Name=LEC; OrderedLocusNames=At3g15356; ORFNames=K7L4.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Herve C., Lescure B.;
RT "Sequence of an Arabidopsis cDNA encoding a legume lectin-like protein.";
RL (er) Plant Gene Register PGR95-095(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION BY SCLAREOL.
RX PubMed=14526118; DOI=10.1104/pp.103.024182;
RA Campbell E.J., Schenk P.M., Kazan K., Penninckx I.A.M.A., Anderson J.P.,
RA Maclean D.J., Cammue B.P.A., Ebert P.R., Manners J.M.;
RT "Pathogen-responsive expression of a putative ATP-binding cassette
RT transporter gene conferring resistance to the diterpenoid sclareol is
RT regulated by multiple defense signaling pathways in Arabidopsis.";
RL Plant Physiol. 133:1272-1284(2003).
RN [7]
RP SUBCELLULAR LOCATION, INDUCTION BY OLIGOGALACTURONIDES, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RX PubMed=18324730; DOI=10.1002/pmic.200700523;
RA Casasoli M., Spadoni S., Lilley K.S., Cervone F., De Lorenzo G., Mattei B.;
RT "Identification by 2-D DIGE of apoplastic proteins regulated by
RT oligogalacturonides in Arabidopsis thaliana.";
RL Proteomics 8:1042-1054(2008).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY MULTIPLE STIMULI.
RC STRAIN=cv. Columbia;
RX PubMed=19214436; DOI=10.1007/s10059-009-0007-1;
RA Lyou S.H., Park H.J., Jung C., Sohn H.B., Lee G., Kim C.H., Kim M.,
RA Choi Y.D., Cheong J.J.;
RT "The Arabidopsis AtLEC gene encoding a lectin-like protein is up-regulated
RT by multiple stimuli including developmental signal, wounding, jasmonate,
RT ethylene, and chitin elicitor.";
RL Mol. Cells 27:75-81(2009).
CC -!- FUNCTION: Plays a role in defense responses triggered by jasmonate,
CC ethylene and chitin. {ECO:0000269|PubMed:19214436}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000269|PubMed:18324730}.
CC -!- TISSUE SPECIFICITY: Expressed in rosette leaves, inflorescences, roots,
CC leaf veins, carpel heads, and silique receptacles.
CC {ECO:0000269|PubMed:19214436}.
CC -!- INDUCTION: By oligogalacturonides and the diterpenoid compound
CC sclareol. Accumulates in response to mechanical wounding, chitin,
CC methyl jasmonate (MeJA) and ethylene (ET) stimuli.
CC {ECO:0000269|PubMed:14526118, ECO:0000269|PubMed:18324730,
CC ECO:0000269|PubMed:19214436}.
CC -!- SIMILARITY: Belongs to the leguminous lectin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA62665.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X91259; CAA62665.1; ALT_FRAME; mRNA.
DR EMBL; AP000413; BAB02166.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75649.1; -; Genomic_DNA.
DR EMBL; AY140015; AAM98157.1; -; mRNA.
DR EMBL; BT020320; AAV85675.1; -; mRNA.
DR EMBL; BT021923; AAX49372.1; -; mRNA.
DR PIR; S71252; S71252.
DR RefSeq; NP_683568.1; NM_148726.3.
DR AlphaFoldDB; Q9LJR2; -.
DR SMR; Q9LJR2; -.
DR BioGRID; 6102; 3.
DR IntAct; Q9LJR2; 2.
DR STRING; 3702.AT3G15356.1; -.
DR PaxDb; Q9LJR2; -.
DR PRIDE; Q9LJR2; -.
DR ProteomicsDB; 238601; -.
DR EnsemblPlants; AT3G15356.1; AT3G15356.1; AT3G15356.
DR GeneID; 820768; -.
DR Gramene; AT3G15356.1; AT3G15356.1; AT3G15356.
DR KEGG; ath:AT3G15356; -.
DR Araport; AT3G15356; -.
DR TAIR; locus:504955770; AT3G15356.
DR eggNOG; ENOG502QRZ3; Eukaryota.
DR HOGENOM; CLU_000288_62_2_1; -.
DR InParanoid; Q9LJR2; -.
DR OMA; YINPIQF; -.
DR OrthoDB; 972171at2759; -.
DR PhylomeDB; Q9LJR2; -.
DR PRO; PR:Q9LJR2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LJR2; baseline and differential.
DR GO; GO:0048046; C:apoplast; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0071323; P:cellular response to chitin; IEP:UniProtKB.
DR GO; GO:0071369; P:cellular response to ethylene stimulus; IEP:UniProtKB.
DR GO; GO:0071395; P:cellular response to jasmonic acid stimulus; IEP:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IDA:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009611; P:response to wounding; IEP:UniProtKB.
DR CDD; cd06899; lectin_legume_LecRK_Arcelin_ConA; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR016363; L-lectin.
DR InterPro; IPR001220; Legume_lectin_dom.
DR Pfam; PF00139; Lectin_legB; 1.
DR PIRSF; PIRSF002690; L-type_lectin_plant; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
PE 1: Evidence at protein level;
KW Apoplast; Ethylene signaling pathway; Glycoprotein;
KW Jasmonic acid signaling pathway; Lectin; Plant defense; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..271
FT /note="Lectin-like protein LEC"
FT /id="PRO_0000428918"
FT REGION 20..270
FT /note="Legume-lectin like"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 151
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 196
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 132
FT /note="N -> S (in Ref. 4; AAM98157)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="S -> N (in Ref. 1; CAA62665)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 271 AA; 29749 MW; B411709DCF58DB9A CRC64;
MQIHKLCFLA LFLANAAFAV KFNFDSFDGS NLLFLGDAEL GPSSDGVSRS GALSMTRDET
PFSHGQGLYI NPIQFKSSNT SSPFDFKTSF TFSITPRTKP NSGQGLAFVI VPAADNSGAS
GGGYLGILNK TNDGKSENNL IFIEFDTFKN NESNDISGNH VGININSMTS LVAEKAGYWV
QTLVGKRKVW SFKDVNLSSG ERFKAWIEFR SKDSRNTITI APENVKKPKR PLIQGSRVLN
DVLLQNMYAG FAGSMGRAGE RHDVWSWSFE N
