LECT2_CAEEL
ID LECT2_CAEEL Reviewed; 335 AA.
AC Q21241; A0A1B1LVF5;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2016, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Leukocyte cell-derived chemotaxin-2 homolog {ECO:0000312|WormBase:K05F1.5};
DE AltName: Full=Chondromodulin II {ECO:0000305|PubMed:27451901};
DE Flags: Precursor;
GN Name=lect-2 {ECO:0000312|WormBase:K05F1.5};
GN ORFNames=K05F1.5 {ECO:0000312|WormBase:K05F1.5};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:ANS57217.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF HIS-67; ALA-74;
RP ILE-101; GLY-133; VAL-153; ASN-244 AND ASP-262.
RX PubMed=27451901; DOI=10.1016/j.cub.2016.07.008;
RA Diaz-Balzac C.A., Rahman M., Lazaro-Pena M.I., Martin Hernandez L.A.,
RA Salzberg Y., Aguirre-Chen C., Kaprielian Z., Buelow H.E.;
RT "Muscle- and Skin-Derived Cues Jointly Orchestrate Patterning of
RT Somatosensory Dendrites.";
RL Curr. Biol. 26:2379-2387(2016).
RN [2] {ECO:0000305}
RP ERRATUM OF PUBMED:27451901.
RX PubMed=27546571; DOI=10.1016/j.cub.2016.07.078;
RA Diaz-Balzac C.A., Rahman M., Lazaro-Pena M.I., Martin Hernandez L.A.,
RA Salzberg Y., Aguirre-Chen C., Kaprielian Z., Buelow H.E.;
RT "Muscle- and Skin-Derived Cues Jointly Orchestrate Patterning of
RT Somatosensory Dendrites.";
RL Curr. Biol. 26:2397-2397(2016).
RN [3] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN COMPLEX WITH DMA-1; MNR-1 AND SAX-7,
RP INTERACTION WITH SAX-7, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND MUTAGENESIS OF GLY-103 AND GLY-259.
RX PubMed=27705746; DOI=10.7554/elife.18345;
RA Zou W., Shen A., Dong X., Tugizova M., Xiang Y.K., Shen K.;
RT "A multi-protein receptor-ligand complex underlies combinatorial dendrite
RT guidance choices in C. elegans.";
RL Elife 5:0-0(2016).
CC -!- FUNCTION: Muscle-derived dendritic guidance cue, which is required for
CC the formation of somatosensory dendritic arbors which extend from PVD
CC and FLP sensory neurons during development (PubMed:27451901). Ligand of
CC a multi-protein dma-1 receptor-ligand complex, which is activated upon
CC binding of lect-2, mnr-1 and sax-7 ligands to control the growth of
CC dendrites that extend anteriorly from the PVD neuronal cell body
CC (PubMed:27451901, PubMed:27705746). Enhances the binding of the mnr-1
CC and sax-7 ligands to the dma-1 receptor-ligand complex
CC (PubMed:27705746). Restricts the growth of secondary PVD dendritic
CC branches and any irregularly positioned ectopic tertiary dendritic
CC branches that originate from secondary branches, and promotes the
CC formation of stable higher order dendritic branches (PubMed:27451901,
CC PubMed:27705746). In particular, it is required for the formation of
CC quaternary PVD dendritic branches and promotes their innervation of
CC body wall muscles (PubMed:27705746). Promotes self-avoidance of
CC tertiary dendritic branches of PVD sensory neurons (PubMed:27451901).
CC Not required for the growth of dendrites that extend from AIY and PVQ
CC interneurons, DVB GABergic neurons, PLM and ALM mechanosensory neurons,
CC AFD sensory neurons and DD/VD and DA/DB motor neurons
CC (PubMed:27451901). {ECO:0000269|PubMed:27451901,
CC ECO:0000269|PubMed:27705746}.
CC -!- SUBUNIT: Component of a multi-protein dma-1 receptor-ligand complex,
CC which is activated upon binding of lect-2, mnr-1 and sax-7 ligands to
CC promote the morphogenesis of dendrites which extend from the PVD
CC neuronal body (PubMed:27705746). Within the complex interacts with sax-
CC 7; the interaction is required for lect-2 dendritic localization and
CC enhances the binding of the mnr-1 and sax-7 ligands to the dma-1
CC receptor-ligand complex (PubMed:27705746).
CC {ECO:0000269|PubMed:27705746}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27451901,
CC ECO:0000269|PubMed:27705746}. Cell junction
CC {ECO:0000269|PubMed:27451901}. Secreted, extracellular space,
CC extracellular matrix, basement membrane {ECO:0000269|PubMed:27451901}.
CC Cell projection, dendrite {ECO:0000269|PubMed:27451901,
CC ECO:0000269|PubMed:27705746}. Perikaryon {ECO:0000269|PubMed:27451901}.
CC Cell surface {ECO:0000269|PubMed:27451901}. Note=Secreted by body wall
CC muscles and taken up by coelomocytes (PubMed:27451901,
CC PubMed:27705746). Localizes to the basement membrane of hypodermal
CC tissues, body wall muscles and along fibrous organelles in larvae and
CC adults (PubMed:27451901). Co-localizes with sax-7 at dendrites
CC (PubMed:27451901, PubMed:27705746). Dendritic localization is dependent
CC on sax-7, but not mnr-1 or dma-1 (PubMed:27451901, PubMed:27705746).
CC {ECO:0000269|PubMed:27451901, ECO:0000269|PubMed:27705746}.
CC -!- TISSUE SPECIFICITY: Expressed in body wall muscle cells, along the
CC boundary of the lateral hypodermis, seam cells, processes of the
CC nervous system including commissures, sensory dendrites in the head,
CC and lateral nerve tracts, and motor neurons and some mechanosensory
CC neurons such as ALM. {ECO:0000269|PubMed:27451901,
CC ECO:0000269|PubMed:27705746}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development
CC (PubMed:27451901). Diffusely expressed from the gastrula stage to the
CC 3-fold embryonic stage (PubMed:27451901). In larvae, expressed in
CC hypodermal tissues and processes of the nervous system, including
CC commissures, sensory dendrites in the head, and lateral nerve tracts
CC (PubMed:27451901). In L3 and L4 stage larvae, mainly expressed in body
CC wall muscles, and it is also expressed in head neurons and ventral
CC nerve cord neurons (PubMed:27705746). {ECO:0000269|PubMed:27451901,
CC ECO:0000269|PubMed:27705746}.
CC -!- SIMILARITY: Belongs to the LECT2/MIM-1 family. {ECO:0000305}.
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DR EMBL; KX255656; ANS57217.1; -; mRNA.
DR EMBL; BX284602; CCD69144.2; -; Genomic_DNA.
DR PIR; T16566; T16566.
DR RefSeq; NP_495141.2; NM_062740.5.
DR AlphaFoldDB; Q21241; -.
DR SMR; Q21241; -.
DR STRING; 6239.K05F1.5; -.
DR EPD; Q21241; -.
DR PaxDb; Q21241; -.
DR PeptideAtlas; Q21241; -.
DR EnsemblMetazoa; K05F1.5.1; K05F1.5.1; WBGene00019407.
DR GeneID; 173978; -.
DR KEGG; cel:CELE_K05F1.5; -.
DR UCSC; K05F1.5; c. elegans.
DR CTD; 173978; -.
DR WormBase; K05F1.5; CE51789; WBGene00019407; lect-2.
DR eggNOG; KOG4377; Eukaryota.
DR GeneTree; ENSGT00390000015484; -.
DR HOGENOM; CLU_046042_0_0_1; -.
DR InParanoid; Q21241; -.
DR OrthoDB; 829237at2759; -.
DR PRO; PR:Q21241; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00019407; Expressed in embryo and 3 other tissues.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005604; C:basement membrane; IDA:UniProtKB.
DR GO; GO:0009986; C:cell surface; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043204; C:perikaryon; IDA:UniProtKB.
DR GO; GO:0071683; C:sensory dendrite; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0060384; P:innervation; IMP:UniProtKB.
DR GO; GO:1903859; P:regulation of dendrite extension; IMP:UniProtKB.
DR GO; GO:0048814; P:regulation of dendrite morphogenesis; IMP:UniProtKB.
DR Gene3D; 2.70.70.10; -; 1.
DR InterPro; IPR011055; Dup_hybrid_motif.
DR InterPro; IPR008663; LECT2.
DR InterPro; IPR016047; Peptidase_M23.
DR PANTHER; PTHR11329; PTHR11329; 1.
DR Pfam; PF01551; Peptidase_M23; 1.
PE 1: Evidence at protein level;
KW Basement membrane; Cell junction; Cell projection; Disulfide bond;
KW Extracellular matrix; Glycoprotein; Metal-binding; Neurogenesis;
KW Reference proteome; Secreted; Signal; Zinc.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..335
FT /note="Leukocyte cell-derived chemotaxin-2 homolog"
FT /id="PRO_5015097204"
FT BINDING 63
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:O14960"
FT CARBOHYD 317
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 28..66
FT /evidence="ECO:0000250|UniProtKB:O14960"
FT DISULFID 39..46
FT /evidence="ECO:0000250|UniProtKB:O14960"
FT MUTAGEN 67
FT /note="H->L: In gk660807; does not cause defects in the
FT formation of the somatosensory dendritic arbor of PVD
FT sensory neurons."
FT /evidence="ECO:0000269|PubMed:27451901"
FT MUTAGEN 74
FT /note="A->V: In gk751884; does not cause defects in the
FT formation of the somatosensory dendritic arbor of PVD
FT sensory neurons."
FT /evidence="ECO:0000269|PubMed:27451901"
FT MUTAGEN 101
FT /note="I->T: In gk609178; defective formation of the
FT somatosensory dendritic arbor of PVD sensory neurons."
FT /evidence="ECO:0000269|PubMed:27451901"
FT MUTAGEN 103
FT /note="G->E: In wy953; defective formation of the
FT somatosensory dendritic arbor of PVD sensory neurons.
FT Secondary dendrites that extend from the PVD neuronal cell
FT body are disorganized and fail to form 'T' shaped tertiary
FT dendrites, and furthermore no quaternary dendritic branches
FT are formed."
FT /evidence="ECO:0000269|PubMed:27705746"
FT MUTAGEN 133
FT /note="G->E: In dz203 and gk606935; defective formation of
FT the somatosensory dendritic arbor of PVD sensory neurons."
FT /evidence="ECO:0000269|PubMed:27451901"
FT MUTAGEN 153
FT /note="V->D: In gk864764; defective formation of the
FT somatosensory dendritic arbor of PVD sensory neurons."
FT /evidence="ECO:0000269|PubMed:27451901"
FT MUTAGEN 244
FT /note="N->K: In gk808537; does not cause defects in the
FT formation of the somatosensory dendritic arbor of PVD
FT sensory neurons."
FT /evidence="ECO:0000269|PubMed:27451901"
FT MUTAGEN 259
FT /note="G->E: In wy935; defective formation of the
FT somatosensory dendritic arbor of PVD sensory neurons.
FT Secondary dendrites that extend from the PVD neuronal cell
FT body are disorganized and fail to form 'T' shaped tertiary
FT dendrites, and furthermore no quaternary dendritic branches
FT are formed."
FT /evidence="ECO:0000269|PubMed:27705746"
FT MUTAGEN 262
FT /note="D->V: In gk762721; does not cause defects in the
FT formation of the somatosensory dendritic arbor of PVD
FT sensory neurons."
FT /evidence="ECO:0000269|PubMed:27451901"
SQ SEQUENCE 335 AA; 37489 MW; A22B9BF7A59ADAB2 CRC64;
MHLRTLHFLI LIGIFIGGQT LGCMKKVCGN NAKNDFRRCP DTDGSCGNYH TERTSGEIID
GVDVRCHLGE PIYAPIEGEM YFWRPYGGKR EKSCADQGVR IEGTGQWQGY AVHISSVKLS
FFGGHVEAGD EIGEALNRYC FNDRGQNDVE PHVEIRLYKE GRLIDPTHHL QNCMCTGQIC
ESNTRNVLLG DPFKTDKRYN GVRGWDVECQ MIDDDDEDSP RAPMIYSPIA GEIVGRIRLF
TDSNGAYTGC DNDGIFIVGI DDWLGFEARL YNVKARADIG FGRKRIIQGE PIATRLACEN
SPDSVFVEIR FEGRVVNITD IITAANCKTP NFPVF
