ID   LEC_BAUPU               Reviewed;         290 AA.
AC   P16030;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 2.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Lectin;
DE   Flags: Precursor;
OS   Bauhinia purpurea (Camel's foot tree) (Phanera purpurea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Cercidoideae; Cercideae; Bauhiniinae;
OC   Bauhinia.
OX   NCBI_TaxID=3806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=1657898; DOI=10.1093/oxfordjournals.jbchem.a123477;
RA   Kusui K., Yamamoto K., Konami Y., Osawa T.;
RT   "cDNA cloning and expression of Bauhinia purpurea lectin.";
RL   J. Biochem. 109:899-903(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 29-62.
RA   Young N.M., Watson D.C., Williams R.E.;
RT   "Lectins and legume chemotaxonomy. Characterisation of the N-acetyl-D-
RT   galactosamine specific lectin of Bauhinia purpurea.";
RL   FEBS Lett. 182:403-405(1985).
CC   -!- FUNCTION: N-acetyl-D-galactosamine specific lectin.
CC   -!- SIMILARITY: Belongs to the leguminous lectin family. {ECO:0000305}.
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DR   EMBL; D12481; BAA02049.1; -; mRNA.
DR   PIR; JX0175; JX0175.
DR   AlphaFoldDB; P16030; -.
DR   SMR; P16030; -.
DR   IntAct; P16030; 1.
DR   iPTMnet; P16030; -.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd06899; lectin_legume_LecRK_Arcelin_ConA; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR016363; L-lectin.
DR   InterPro; IPR000985; Lectin_LegA_CS.
DR   InterPro; IPR019825; Lectin_legB_Mn/Ca_BS.
DR   InterPro; IPR001220; Legume_lectin_dom.
DR   Pfam; PF00139; Lectin_legB; 1.
DR   PIRSF; PIRSF002690; L-type_lectin_plant; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   PROSITE; PS00308; LECTIN_LEGUME_ALPHA; 1.
DR   PROSITE; PS00307; LECTIN_LEGUME_BETA; 1.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Glycoprotein; Lectin; Manganese;
KW   Metal-binding; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000269|Ref.2"
FT   CHAIN           29..290
FT                   /note="Lectin"
FT                   /id="PRO_0000017584"
FT   BINDING         161
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         163
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         163
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         165
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         167
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         172
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         172
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         177
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        48
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:1657898"
FT   CARBOHYD        57
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:1657898"
FT   CARBOHYD        183
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        255
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        270
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        35
FT                   /note="G -> S (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        55
FT                   /note="Missing (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        62
FT                   /note="P -> W (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   290 AA;  32238 MW;  9DBC144630D2C768 CRC64;
     MLLYNSKSYV LQLIFITLLL TQLNKVKSTS STLTGFTFPN FWSNTQENGT EIIFLGNATY
     TPGALRLTRI GEDGIPLKSN AGQASYSRPV FLWDSTGHVA SFYTSFSFIV RSIDVPHITA
     DGFAFFLAPV DSSVKDYGGC LGLFRYKTAT DPSKNQVVAV EFDTWPNTEW SDLRYPHIGI
     NVNSTVSVAT TRWDNDDAYV TKSTAHITYD ATSKIITVLL TYDNGRHYQL SHVVDLPKIL
     PERVRIGFSG GTGFNETQYI LSWSFTSTLN STKISALTQK LRSSASYSSM