LEC_MORIN
ID LEC_MORIN Reviewed; 157 AA.
AC C0HK14;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2016, sequence version 1.
DT 25-MAY-2022, entry version 10.
DE RecName: Full=Galactose-specific lectin {ECO:0000305|PubMed:27664852};
DE AltName: Full=Jacalin-related lectin {ECO:0000303|PubMed:27664852};
DE AltName: Full=Mulberry latex galactose-specific lectin {ECO:0000303|PubMed:27664852};
DE Short=MLGL {ECO:0000303|PubMed:27664852};
DE Contains:
DE RecName: Full=Galactose-specific lectin, light chain 1 {ECO:0000305|PubMed:27664852};
DE Contains:
DE RecName: Full=Galactose-specific lectin, light chain 2 {ECO:0000305|PubMed:27664852};
DE Contains:
DE RecName: Full=Galactose-specific lectin, heavy chain {ECO:0000305|PubMed:27664852};
DE Flags: Fragments;
OS Morus indica (Mulberry).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Moraceae; Morus.
OX NCBI_TaxID=248361 {ECO:0000303|PubMed:27664852};
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, SUBUNIT, MASS SPECTROMETRY, GLYCOSYLATION AT
RP ASN-45, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Latex {ECO:0000303|PubMed:27664852};
RX PubMed=27664852; DOI=10.1016/j.abb.2016.09.009;
RA Datta D., Pohlentz G., Schulte M., Kaiser M., Goycoolea F.M., Muething J.,
RA Mormann M., Swamy M.J.;
RT "Physico-chemical characteristics and primary structure of an affinity-
RT purified alpha-D-galactose-specific, jacalin-related lectin from the latex
RT of mulberry (Morus indica).";
RL Arch. Biochem. Biophys. 609:59-68(2016).
CC -!- FUNCTION: Alpha-D-galactose-specific lectin. Has hemagglutinating
CC activity towards human and rabbit erythrocytes. Is highly cytotoxic to
CC human cells in vitro. {ECO:0000269|PubMed:27664852}.
CC -!- SUBUNIT: Tetramer of heterodimers of light and heavy chains which are
CC non-covalently linked. {ECO:0000269|PubMed:27664852}.
CC -!- PTM: N-linked carbohydrates at Asn-45 can be of complex or paucimannose
CC type. {ECO:0000269|PubMed:27664852}.
CC -!- MASS SPECTROMETRY: [Galactose-specific lectin, heavy chain]:
CC Mass=15761; Method=Electrospray; Note=Heavy chain.;
CC Evidence={ECO:0000269|PubMed:27664852};
CC -!- MASS SPECTROMETRY: [Galactose-specific lectin, light chain 1]:
CC Mass=2163; Method=Electrospray; Note=Light chain 1.;
CC Evidence={ECO:0000269|PubMed:27664852};
CC -!- MASS SPECTROMETRY: [Galactose-specific lectin, light chain 2]:
CC Mass=2475; Method=Electrospray; Note=Light chain 2.;
CC Evidence={ECO:0000269|PubMed:27664852};
CC -!- SIMILARITY: Belongs to the jacalin lectin family. {ECO:0000255|PROSITE-
CC ProRule:PRU01088}.
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DR AlphaFoldDB; C0HK14; -.
DR SMR; C0HK14; -.
DR iPTMnet; C0HK14; -.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR CDD; cd09612; Jacalin; 1.
DR Gene3D; 2.100.10.30; -; 1.
DR InterPro; IPR001229; Jacalin-like_lectin_dom.
DR InterPro; IPR033734; Jacalin-like_lectin_dom_plant.
DR InterPro; IPR036404; Jacalin-like_lectin_dom_sf.
DR Pfam; PF01419; Jacalin; 1.
DR SMART; SM00915; Jacalin; 1.
DR SUPFAM; SSF51101; SSF51101; 1.
DR PROSITE; PS51752; JACALIN_LECTIN; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Lectin.
FT CHAIN 1..24
FT /note="Galactose-specific lectin, light chain 1"
FT /evidence="ECO:0000269|PubMed:27664852"
FT /id="PRO_0000438384"
FT CHAIN 3..24
FT /note="Galactose-specific lectin, light chain 2"
FT /evidence="ECO:0000269|PubMed:27664852"
FT /id="PRO_0000438385"
FT CHAIN 25..157
FT /note="Galactose-specific lectin, heavy chain"
FT /evidence="ECO:0000269|PubMed:27664852"
FT /id="PRO_0000438386"
FT DOMAIN 12..157
FT /note="Jacalin-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01088"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498,
FT ECO:0000269|PubMed:27664852"
FT UNSURE 2
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 5
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 6
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 9
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 13
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 36
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 39
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 49
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 52
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 53
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 66
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 67
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 72
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 79
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 80
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 82
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 89
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 98
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 103
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 108
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 111
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 114
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 129
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 130
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 132
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 133
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 136
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 137
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 141
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 148
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 151
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 155
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:27664852"
FT UNSURE 157
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:27664852"
FT NON_CONS 24..25
FT /evidence="ECO:0000269|PubMed:27664852"
SQ SEQUENCE 157 AA; 17059 MW; F0767EF8461F5F6F CRC64;
AKNNQQSGKS QSIVVGTWGA EVTSGVAFDD GSYTGIREIN FEYNNETAIG SIQVTYDVDG
TPFEAKKHAS FITGFTPVKI SLDFPSEYIV EVSGYTGKVS GYLVVRSLTF KTNKETYGPY
GVTSGTHFKL PIQDGLIVGF KGSVGYWLDY IGFHLAL
