ID   LEDGE_XENLA             Reviewed;         121 AA.
AC   Q8QGU6;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Protein ripply2.1;
DE   AltName: Full=Protein ledgerline;
GN   Name=ripply2.1; Synonyms=ledgerline;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:BAB90857.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX   PubMed=16971787; DOI=10.2108/zsj.23.689;
RA   Chan T., Satow R., Kitagawa H., Kato S., Asashima M.;
RT   "Ledgerline, a novel Xenopus laevis gene, regulates differentiation of
RT   presomitic mesoderm during somitogenesis.";
RL   Zool. Sci. 23:689-697(2006).
RN   [2]
RP   FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=19247927; DOI=10.1387/ijdb.082823kh;
RA   Hitachi K., Danno H., Tazumi S., Aihara Y., Uchiyama H., Okabayashi K.,
RA   Kondow A., Asashima M.;
RT   "The Xenopus Bowline/Ripply family proteins negatively regulate the
RT   transcriptional activity of T-box transcription factors.";
RL   Int. J. Dev. Biol. 53:631-639(2009).
CC   -!- FUNCTION: Required during somitogenesis to regulate somite
CC       differentiation and the positioning of the presomitic mesoderm-front.
CC       Represses the expression of genes involved in somite segmentation by
CC       acting with the corepressor tle4 to down-regulate the transcriptional
CC       activity of tbx6. Also regulates retinoic acid signaling during
CC       somitogenesis and is necessary for the expression of aldh1a2/raldh2.
CC       {ECO:0000269|PubMed:16971787, ECO:0000269|PubMed:19247927}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16971787}.
CC   -!- TISSUE SPECIFICITY: Expressed in the presomitic mesoderm (PSM) in the
CC       anterior halves of somitomeres S-0, S-I and S-II and in the newly
CC       formed somites. {ECO:0000269|PubMed:16971787,
CC       ECO:0000269|PubMed:19247927}.
CC   -!- DEVELOPMENTAL STAGE: Expressed from stage 12.
CC       {ECO:0000269|PubMed:16971787}.
CC   -!- INDUCTION: By notch signaling. By tbx6 together with thy1 and tcf3.
CC       {ECO:0000269|PubMed:16971787, ECO:0000269|PubMed:19247927}.
CC   -!- DOMAIN: The ripply homology domain is required for transcriptional
CC       repression. {ECO:0000250|UniProtKB:Q25QX6}.
CC   -!- DOMAIN: The WRPW motif is required for binding to tle/groucho proteins
CC       and transcriptional repression. {ECO:0000250|UniProtKB:Q25QX6}.
CC   -!- SIMILARITY: Belongs to the ripply family.
CC       {ECO:0000250|UniProtKB:Q25QX6}.
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DR   EMBL; AB073615; BAB90857.1; -; mRNA.
DR   RefSeq; NP_001082224.1; NM_001088755.1.
DR   AlphaFoldDB; Q8QGU6; -.
DR   GeneID; 398303; -.
DR   KEGG; xla:398303; -.
DR   CTD; 398303; -.
DR   Xenbase; XB-GENE-6251611; ripply2.1.S.
DR   OrthoDB; 1552301at2759; -.
DR   Proteomes; UP000186698; Chromosome 5S.
DR   Bgee; 398303; Expressed in testis and 6 other tissues.
DR   GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR   GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR   GO; GO:0001756; P:somitogenesis; IMP:UniProtKB.
DR   InterPro; IPR028127; Ripply_fam.
DR   PANTHER; PTHR16770; PTHR16770; 1.
DR   Pfam; PF14998; Ripply; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Nucleus; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..121
FT                   /note="Protein ripply2.1"
FT                   /id="PRO_0000308375"
FT   REGION          1..69
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          69..104
FT                   /note="Ripply homology domain"
FT                   /evidence="ECO:0000255"
FT   MOTIF           29..32
FT                   /note="WRPW motif"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..25
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        40..62
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   121 AA;  13938 MW;  51324E674EB312BE CRC64;
     MEPNQQRSCG SSSDSSATGT PQGSNVAVWR PWHDAPRTHV QNPPTAQQQF YSDNQSHMND
     KGKPPSFQHP VKLFWPKSRC YDFMYQEAEE LLRHFPVQAT ISLYQETDSD SDSEEEDIYE
     N