ID   LEF2_NPVAC              Reviewed;         210 AA.
AC   P41418;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   23-FEB-2022, entry version 63.
DE   RecName: Full=Primase-associated factor LEF-2;
GN   Name=LEF-2; ORFNames=ORF6;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=1926775; DOI=10.1016/0042-6822(91)90770-c;
RA   Possee R.D., Sun T.P., Howard S.C., Ayres M.D., Hill-Perkins M.,
RA   Gearing K.L.;
RT   "Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4
RT   kbp EcoRI-I and -R (polyhedrin gene) region.";
RL   Virology 185:229-241(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [3]
RP   FUNCTION.
RX   PubMed=8445724; DOI=10.1128/jvi.67.4.2149-2158.1993;
RA   Passarelli A.L., Miller L.K.;
RT   "Three baculovirus genes involved in late and very late gene expression:
RT   ie-1, ie-n, and lef-2.";
RL   J. Virol. 67:2149-2158(1993).
RN   [4]
RP   FUNCTION, INTERACTION WITH DNA PRIMASE, AND SUBCELLULAR LOCATION.
RX   PubMed=9060674; DOI=10.1128/jvi.71.4.3114-3119.1997;
RA   Evans J.T., Leisy D.J., Rohrmann G.F.;
RT   "Characterization of the interaction between the baculovirus replication
RT   factors LEF-1 and LEF-2.";
RL   J. Virol. 71:3114-3119(1997).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=20219928; DOI=10.1128/jvi.02423-09;
RA   Wu C.P., Huang Y.J., Wang J.Y., Wu Y.L., Lo H.R., Wang J.C., Chao Y.C.;
RT   "Autographa californica multiple nucleopolyhedrovirus LEF-2 is a capsid
RT   protein required for amplification but not initiation of viral DNA
RT   replication.";
RL   J. Virol. 84:5015-5024(2010).
CC   -!- FUNCTION: Plays an essential role in viral DNA replication. Does not
CC       seem to participate in the initiation step but is rather important for
CC       the amplification of DNA. Also required for expression from the vp39
CC       and polh promoters. {ECO:0000269|PubMed:20219928,
CC       ECO:0000269|PubMed:8445724, ECO:0000269|PubMed:9060674}.
CC   -!- SUBUNIT: Interacts with the DNA primase. {ECO:0000269|PubMed:9060674}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:20219928}. Host
CC       nucleus {ECO:0000269|PubMed:20219928}. Host cytoplasm
CC       {ECO:0000269|PubMed:20219928}. Note=Colocalizes with the active viral
CC       DNA replication sites in the nucleus of infected cells.
CC       {ECO:0000269|PubMed:9060674}.
CC   -!- SIMILARITY: Belongs to the baculoviridae LEF-2 family. {ECO:0000305}.
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DR   EMBL; M75679; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; L22858; AAA66636.1; -; Genomic_DNA.
DR   PIR; F72850; F72850.
DR   RefSeq; NP_054035.1; NC_001623.1.
DR   GeneID; 1403838; -.
DR   KEGG; vg:1403838; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IDA:UniProtKB.
DR   GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IDA:UniProtKB.
DR   GO; GO:0019079; P:viral genome replication; IDA:UniProtKB.
DR   GO; GO:0019083; P:viral transcription; IEA:InterPro.
DR   InterPro; IPR004283; Lef-2.
DR   Pfam; PF03041; Baculo_LEF-2; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Host cytoplasm; Host nucleus; Reference proteome;
KW   Transcription; Transcription regulation; Virion.
FT   CHAIN           1..210
FT                   /note="Primase-associated factor LEF-2"
FT                   /id="PRO_0000132818"
SQ   SEQUENCE   210 AA;  23926 MW;  A3BC524B24145B78 CRC64;
     MANASYNVWS PLIRASCLDK KATYLIDPDD FIDKLTLTPY TVFYNGGVLV KISGLRLYML
     LTAPPTINEI KNSNFKKRSK RNICMKECVE GKKNVVDMLN NKINMPPCIK KILNDLKENN
     VPRGGMYRKR FILNCYIANV VSCAKCENRC LIKALTHFYN HDSKCVGEVM HLLIKSQDVY
     KPPNCQKMKT VDKLCPFAGN CKGLNPICNY