LEF4_NPVAC
ID LEF4_NPVAC Reviewed; 464 AA.
AC P41477;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=DNA-dependent RNA polymerase subunit LEF-4;
DE EC=2.7.7.50;
GN Name=LEF-4; ORFNames=ORF90;
OS Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX NCBI_TaxID=46015;
OH NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=L1;
RX PubMed=8249293; DOI=10.1006/viro.1993.1646;
RA Passarelli A.L., Miller L.K.;
RT "Identification of genes encoding late expression factors located between
RT 56.0 and 65.4 map units of the Autographa californica nuclear polyhedrosis
RT virus genome.";
RL Virology 197:704-714(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C6;
RX PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT virus.";
RL Virology 202:586-605(1994).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=9499802; DOI=10.1006/viro.1997.8971;
RA Durantel D., Croizier G., Ravallec M., Lopez-Ferber M.;
RT "Temporal expression of the AcMNPV lef-4 gene and subcellular localization
RT of the protein.";
RL Virology 241:276-284(1998).
RN [4]
RP INTERACTION WITH LEF-8; LEF-9 AND P47, AND FUNCTION.
RX PubMed=9733837; DOI=10.1128/jvi.72.10.7985-7991.1998;
RA Guarino L.A., Xu B., Jin J., Dong W.;
RT "A virus-encoded RNA polymerase purified from baculovirus-infected cells.";
RL J. Virol. 72:7985-7991(1998).
RN [5]
RP FUNCTION.
RX PubMed=9811740; DOI=10.1128/jvi.72.12.10020-10028.1998;
RA Gross C.H., Shuman S.;
RT "RNA 5'-triphosphatase, nucleoside triphosphatase, and guanylyltransferase
RT activities of baculovirus LEF-4 protein.";
RL J. Virol. 72:10020-10028(1998).
RN [6]
RP FUNCTION.
RX PubMed=9811738; DOI=10.1128/jvi.72.12.10003-10010.1998;
RA Guarino L.A., Jin J., Dong W.;
RT "Guanylyltransferase activity of the LEF-4 subunit of baculovirus RNA
RT polymerase.";
RL J. Virol. 72:10003-10010(1998).
CC -!- FUNCTION: Component of the viral DNA-dependent RNA polymerase that
CC catalyzes two reactions involved in viral RNA cap formation: an RNA 5'-
CC triphosphatase that hydrolyzes the gamma phosphate of triphosphate-
CC terminated RNA and a guanylyltransferase that reacts with GTP to form a
CC covalent protein-guanylate adduct. Therefore plays an essential role in
CC late and very late gene expression. {ECO:0000269|PubMed:8249293,
CC ECO:0000269|PubMed:9733837, ECO:0000269|PubMed:9811738,
CC ECO:0000269|PubMed:9811740}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-
CC end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + diphosphate;
CC Xref=Rhea:RHEA:67012, Rhea:RHEA-COMP:17165, Rhea:RHEA-COMP:17166,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:167616, ChEBI:CHEBI:167617; EC=2.7.7.50;
CC -!- SUBUNIT: Interacts with LEF-8, LEF-9, and p47.
CC {ECO:0000269|PubMed:9733837}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:9499802}. Host
CC nucleus {ECO:0000269|PubMed:9499802}. Note=Preferentially in the host
CC nucleus and associated with the virogenic stroma.
CC {ECO:0000269|PubMed:9499802}.
CC -!- SIMILARITY: Belongs to the baculoviridae LEF-4 family. {ECO:0000305}.
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DR EMBL; L22858; AAA66720.1; -; Genomic_DNA.
DR EMBL; L20217; AAA46713.1; -; Genomic_DNA.
DR PIR; C72861; C72861.
DR RefSeq; NP_054120.1; NC_001623.1.
DR PRIDE; P41477; -.
DR GeneID; 1403923; -.
DR KEGG; vg:1403923; -.
DR Proteomes; UP000008292; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0004484; F:mRNA guanylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0039695; P:DNA-templated viral transcription; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR007790; LEF-4.
DR Pfam; PF05098; LEF-4; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host nucleus; Reference proteome; Transcription;
KW Transcription regulation; Transferase.
FT CHAIN 1..464
FT /note="DNA-dependent RNA polymerase subunit LEF-4"
FT /id="PRO_0000132824"
SQ SEQUENCE 464 AA; 53910 MW; 07B1673CA0205986 CRC64;
MDYGDFVIEK EISYSINFSQ DLLYKILNSY IVPNYSLAQQ YFDLYDENGF RTRIPIQSAC
NNIISSVKKT NSKHKKFVYW PKDTNALVPL VWRESKEIKL PYKTLSHNLS KIIKVYVYQH
DKIEIKFEHV YFSKSDIDLF DSTMANKISK LLTLLENGDA SETLQNSQVG SDEILARIRL
EYEFDDDAPD DAQLNVMCNI IADMEALTDA QNISPFVPLT TLIDKMAPRK FEREQKIVYG
DDAFDNASVK KWALKLDGMR GRGLFMRNFC IIQTDDMQFY KTKMANLFAL NNIVAFQCEV
MDKQKIYITD LLQVFKYKYN NRTQYECGVN ASYAIDPVTA IECINYMNNN VQSVTLTDTC
PAIELRFQQF FDPPLQQSNY MTVSVDGYVV LDTELRYVKY KWMPTTELEY DAVNKSFNTL
NGPLNGLMIL TDLPELLHEN IYECVITDTT INVLKHRRDR IVPN