ID   LEF5_NPVAC              Reviewed;         265 AA.
AC   P41658;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   29-SEP-2021, entry version 64.
DE   RecName: Full=Late expression factor 5;
GN   Name=LEF-5;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HR3;
RX   PubMed=1994581; DOI=10.1016/0042-6822(91)90500-b;
RA   Lu A., Carstens E.B.;
RT   "Nucleotide sequence of a gene essential for viral DNA replication in the
RT   baculovirus Autographa californica nuclear polyhedrosis virus.";
RL   Virology 181:336-347(1991).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=8249293; DOI=10.1006/viro.1993.1646;
RA   Passarelli A.L., Miller L.K.;
RT   "Identification of genes encoding late expression factors located between
RT   56.0 and 65.4 map units of the Autographa californica nuclear polyhedrosis
RT   virus genome.";
RL   Virology 197:704-714(1993).
RN   [4]
RP   SUBUNIT.
RX   PubMed=9770426; DOI=10.1006/viro.1998.9334;
RA   Harwood S.H., Li L., Ho P.S., Preston A.K., Rohrmann G.F.;
RT   "AcMNPV late expression factor-5 interacts with itself and contains a zinc
RT   ribbon domain that is required for maximal late transcription activity and
RT   is homologous to elongation factor TFIIS.";
RL   Virology 250:118-134(1998).
RN   [5]
RP   FUNCTION.
RX   PubMed=12438592; DOI=10.1128/jvi.76.24.12663-12675.2002;
RA   Guarino L.A., Dong W., Jin J.;
RT   "In vitro activity of the baculovirus late expression factor LEF-5.";
RL   J. Virol. 76:12663-12675(2002).
RN   [6]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=21601232; DOI=10.1016/j.virol.2011.04.019;
RA   Su J., Lung O., Blissard G.W.;
RT   "The Autographa californica multiple nucleopolyhedrovirus lef-5 gene is
RT   required for productive infection.";
RL   Virology 416:54-64(2011).
CC   -!- FUNCTION: Plays a role in the transcription of late viral genes. May
CC       function as an transcriptional initiation factor.
CC       {ECO:0000269|PubMed:12438592, ECO:0000269|PubMed:21601232}.
CC   -!- SUBUNIT: Interacts (via N-terminus) with itself.
CC       {ECO:0000269|PubMed:9770426}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:21601232}.
CC       Note=Co-localizes with IE1 in the nucleus of infected cells.
CC       {ECO:0000269|PubMed:21601232}.
CC   -!- SIMILARITY: Belongs to the baculoviridae LEF-5 family. {ECO:0000305}.
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DR   EMBL; L22858; AAA66729.1; -; Genomic_DNA.
DR   EMBL; M57687; AAA67906.1; -; Genomic_DNA.
DR   PIR; D72862; D72862.
DR   RefSeq; NP_054129.1; NC_001623.1.
DR   GeneID; 1403932; -.
DR   KEGG; vg:1403932; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039695; P:DNA-templated viral transcription; IDA:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR021758; Baculo_LEF5_C.
DR   InterPro; IPR006923; Baculo_LEF5_N.
DR   Pfam; PF04838; Baculo_LEF5; 1.
DR   Pfam; PF11792; Baculo_LEF5_C; 1.
PE   1: Evidence at protein level;
KW   Host nucleus; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..265
FT                   /note="Late expression factor 5"
FT                   /id="PRO_0000132826"
FT   CONFLICT        127..147
FT                   /note="GVDCCCPCQLLHKSLLNTKNY -> ASIAVVRASYCTRVYSTPKIN (in
FT                   Ref. 2; AAA67906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        229
FT                   /note="S -> N (in Ref. 2; AAA67906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        263
FT                   /note="S -> F (in Ref. 2; AAA67906)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   265 AA;  31010 MW;  6CC3C88409868578 CRC64;
     MSFDDGVVKA QTDPFALKRG GHNVQKWTSY ALFKLFKEFR INKNYSKLID FLTENFPNNV
     KNKTFNFSST GHLFHSLHAY VPSVSDLVKE RKQIRLQTEY LAKLFNNTIN DFKLYTELYE
     FIERTEGVDC CCPCQLLHKS LLNTKNYVEN LNCKLFDIKP PKFKKEPFDN ILYKYSLNYK
     SLLLKKKEKH TSTGCTRKKK IKHRQILNDK VIYLQNSNKN KLFELSGLSL KSCRHDFVTV
     ESQTRAGDEI ASFIRYCRLC GMSGC