ID   LEF8_NPVAC              Reviewed;         876 AA.
AC   P41452;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Probable DNA-directed RNA polymerase catalytic subunit;
DE            EC=2.7.7.6;
DE   AltName: Full=Late expression factor 8;
GN   Name=LEF-8;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=L1;
RX   PubMed=8207843; DOI=10.1128/jvi.68.7.4673-4678.1994;
RA   Passarelli A.L., Todd J.W., Miller L.K.;
RT   "A baculovirus gene involved in late gene expression predicts a large
RT   polypeptide with a conserved motif of RNA polymerases.";
RL   J. Virol. 68:4673-4678(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [3]
RP   INTERACTION WITH LEF-4; LEF-9 AND P47, AND FUNCTION.
RX   PubMed=9733837; DOI=10.1128/jvi.72.10.7985-7991.1998;
RA   Guarino L.A., Xu B., Jin J., Dong W.;
RT   "A virus-encoded RNA polymerase purified from baculovirus-infected cells.";
RL   J. Virol. 72:7985-7991(1998).
CC   -!- FUNCTION: Component of the viral DNA-dependent RNA polymerase which is
CC       composed of four equimolar subunits of LEF-4, LEF-8, LEF-9, and p47.
CC       Plays an essential role in late and very late gene expression.
CC       {ECO:0000269|PubMed:9733837}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: Interacts with LEF-4, LEF-9, and p47.
CC       {ECO:0000269|PubMed:9733837}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; L22858; AAA66680.1; -; Genomic_DNA.
DR   EMBL; U04879; AAA20054.1; -; Genomic_DNA.
DR   PIR; B72856; B72856.
DR   RefSeq; NP_054079.1; NC_001623.1.
DR   GeneID; 1403882; -.
DR   KEGG; vg:1403882; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0039695; P:DNA-templated viral transcription; IDA:UniProtKB.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 1.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR007025; LEF-8.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   Pfam; PF04941; LEF-8; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   1: Evidence at protein level;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transcription regulation; Transferase.
FT   CHAIN           1..876
FT                   /note="Probable DNA-directed RNA polymerase catalytic
FT                   subunit"
FT                   /id="PRO_0000048053"
SQ   SEQUENCE   876 AA;  101765 MW;  F129466336F9C3D2 CRC64;
     MTDVVQDFNE LYDRIENKYK LKYTFDCATN SNERMLFCAI QERKSYLCCA LNEQLSCVMH
     KCVPVIFGTR LDKQFRETDD VDANNNINGT FMLDGRFLSF PNIMMNNNVL VHNFYDKLYA
     KHCKRMFLYG NVDQEKHINR AIQLVYDKQD DVLFARDVYA SDYVVTEDLN SVLETYLANS
     GKWKPLDFLF EYNTLHKQQL VEHIKIIMNH DINYSIDSLA NKIVYKHAYL IELLLTSTIL
     QNYQRVLDKT ADDDAYTVAN KRRKIQSVLY NKESKKIVDC IVNGRLIYCV SKTFSKQRKV
     FPNQQDNSSN NNIEISLPVL KYRVGNEVAR ITNDSMRQKM LKQKKDFVKF IGSFFHGEMT
     VAGKKFFLCR NACLPNVDYE MVAQKFQYLL KHNLVAFVDD LNDVQDDSLL IAFNDRPTNL
     KCLKSNTSFI VYTMKRNMAP IELKITDRIL YVNHHEGMIC IKKKLRVNNE ADINVLLTPY
     EYHYKHSIYY NPIIQCTIVE NDDVKSLMSK LEQYYYRNFI HLFHTTPVPK LIVSLTNLKN
     AMPVFEYKEE NCVSGLPNGY SVAVNKSILL NNKMFKLWTL VRDNKLMTAE DPYIPHIALP
     ICLYNNKVNK LKGKLVVGPK QSCLVKFTNS SDKNYVALDD GLVLYMAGVL VSNAKINWVY
     DGRRYKIETC TNGNFNVYKV YVYFRQIKNQ KIEKLDASMV VNGDNVMLKI VIVTSTNDLE
     GIKICGIHGQ KGVFNRSEDL TEWMAEDGTH AQICLSPVSF LSRQSNFDKI ERKYVVRGGN
     HDDPHAKRYP IFNIPYMLFN NTPDNIFKEF IKTSHTGHEK VEGTRFDQWT KNQSFVGNRM
     SESLHWMRGG SNLPQNCGEF NVVSSLLMCN NTIMKN