LEG1A_DANRE
ID LEG1A_DANRE Reviewed; 364 AA.
AC A5PF61;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Protein leg1a {ECO:0000303|PubMed:21857963};
DE Short=Leg1-A {ECO:0000303|PubMed:21857963};
DE AltName: Full=Liver-enriched gene protein 1-A {ECO:0000303|PubMed:21857963};
DE Flags: Precursor;
GN Name=leg1a {ECO:0000303|PubMed:21857963};
GN ORFNames=zgc:172246 {ECO:0000312|Ensembl:ENSDARP00000064418};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Ensembl:ENSDARP00000064418};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=21857963; DOI=10.1371/journal.pone.0022910;
RA Chang C., Hu M., Zhu Z., Lo L.J., Chen J., Peng J.;
RT "liver-enriched gene 1a and 1b encode novel secretory proteins essential
RT for normal liver development in zebrafish.";
RL PLoS ONE 6:E22910-E22910(2011).
CC -!- FUNCTION: Important for early development of liver, exocrine pancreas
CC and intestine, probably through cell cycle regulation. In liver, its
CC function is partially redundant with leg1b function.
CC {ECO:0000269|PubMed:21857963}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21857963}.
CC -!- TISSUE SPECIFICITY: Detected in all tissues tested, with the highest
CC levels in serum (at protein level). At mRNA level, only expressed in
CC liver. {ECO:0000269|PubMed:21857963}.
CC -!- DEVELOPMENTAL STAGE: Expressed as early as 24 hours post fecondation
CC (hpf), expressed at higher levels than leg1b.
CC {ECO:0000269|PubMed:21857963}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein causes a
CC small liver phenotype. Morpholino which can simultaneously block the
CC translation of leg1a and leg1b, causes a more severe small liver
CC phenotype with hypoplastic exocrine pancreas and intestinal tube as
CC well. {ECO:0000269|PubMed:21857963}.
CC -!- SIMILARITY: Belongs to the LEG1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL954182; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC155632; AAI55633.1; -; mRNA.
DR RefSeq; NP_001093526.1; NM_001100056.2.
DR AlphaFoldDB; A5PF61; -.
DR SMR; A5PF61; -.
DR STRING; 7955.ENSDARP00000064418; -.
DR PaxDb; A5PF61; -.
DR PeptideAtlas; A5PF61; -.
DR Ensembl; ENSDART00000064419; ENSDARP00000064418; ENSDARG00000090722.
DR GeneID; 796447; -.
DR KEGG; dre:796447; -.
DR CTD; 796447; -.
DR ZFIN; ZDB-GENE-030131-1178; leg1.1.
DR eggNOG; ENOG502R60Y; Eukaryota.
DR GeneTree; ENSGT00390000004904; -.
DR HOGENOM; CLU_071068_0_0_1; -.
DR InParanoid; A5PF61; -.
DR OrthoDB; 1401349at2759; -.
DR PhylomeDB; A5PF61; -.
DR TreeFam; TF332991; -.
DR PRO; PR:A5PF61; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 20.
DR Bgee; ENSDARG00000090722; Expressed in liver and 16 other tissues.
DR GO; GO:0005615; C:extracellular space; IDA:ZFIN.
DR GO; GO:0055123; P:digestive system development; IMP:ZFIN.
DR GO; GO:1990402; P:embryonic liver development; IMP:ZFIN.
DR GO; GO:0001889; P:liver development; IMP:ZFIN.
DR InterPro; IPR008499; Leg1.
DR PANTHER; PTHR18820; PTHR18820; 1.
DR Pfam; PF05612; Leg1; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..364
FT /note="Protein leg1a"
FT /id="PRO_0000432356"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 364 AA; 39932 MW; BE4FF6CEC1A713E2 CRC64;
MSEMGFLRSV AAVLLLAVFS HAAVVTENGL PIQWGKAPSD LSHLPISDTG VQVNPWDYSQ
RMTMYKMLIN ATNAYMSSMG PGEQENPLWS LPLQLGWKLK SGRLADPTLD SSSTCGSEAS
DPVCISPLSW FACVNYYLSV LPFLAAVETG VVSSGGHQVL IQVPAEVAQD YCSSYSDCST
KHPNAMAKWH LFFQSLRQVS QSEDSDFNKK DSILGLMWAA EEESLQTASG ACTERQKLYS
SPEVSFQQSW LNSAAFVSAA HFHANIERSE KFMAPLPSRV LQEADSPPNI ADLSTEENHT
LYIFGWMNSV NQLLGGSLVN LWRKAMCSAQ AREKGQALLH DLILDPKFPG SSLWSILSEM
STSC
