ID   LEG1B_DANRE             Reviewed;         364 AA.
AC   Q4QRF7; Q6P0T9;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-MAR-2015, sequence version 2.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Protein leg1b {ECO:0000303|PubMed:21857963};
DE            Short=Leg1-B {ECO:0000303|PubMed:21857963};
DE   AltName: Full=Liver-enriched gene protein 1-B {ECO:0000303|PubMed:21857963};
DE   Flags: Precursor;
GN   Name=leg1b {ECO:0000303|PubMed:21857963}; ORFNames=zgc:77778;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB; TISSUE=Liver;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=21857963; DOI=10.1371/journal.pone.0022910;
RA   Chang C., Hu M., Zhu Z., Lo L.J., Chen J., Peng J.;
RT   "liver-enriched gene 1a and 1b encode novel secretory proteins essential
RT   for normal liver development in zebrafish.";
RL   PLoS ONE 6:E22910-E22910(2011).
CC   -!- FUNCTION: Involved in early development of liver, exocrine pancreas and
CC       intestine, probably through cell cycle regulation. In liver, its
CC       function is partially redundant with leg1a function.
CC       {ECO:0000269|PubMed:21857963}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21857963}.
CC   -!- TISSUE SPECIFICITY: Detected in all tissues tested, with the highest
CC       levels in serum (at protein level). At mRNA level, only expressed in
CC       liver. {ECO:0000269|PubMed:21857963}.
CC   -!- DEVELOPMENTAL STAGE: Expressed as early as 24 hours post fecondation
CC       (hpf), expressed at lower levels than leg1a.
CC   -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein causes a
CC       small liver phenotype. Morpholino which can simultaneously block the
CC       translation of leg1a and leg1b, causes a more severe small liver
CC       phenotype with hypoplastic exocrine pancreas and intestinal tube as
CC       well. {ECO:0000269|PubMed:21857963}.
CC   -!- SIMILARITY: Belongs to the LEG1 family. {ECO:0000305}.
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DR   EMBL; BC065450; AAH65450.1; -; mRNA.
DR   EMBL; BC097023; AAH97023.1; -; mRNA.
DR   RefSeq; NP_998368.1; NM_213203.1.
DR   AlphaFoldDB; Q4QRF7; -.
DR   SMR; Q4QRF7; -.
DR   STRING; 7955.ENSDARP00000016639; -.
DR   PaxDb; Q4QRF7; -.
DR   PeptideAtlas; Q4QRF7; -.
DR   GeneID; 406484; -.
DR   KEGG; dre:406484; -.
DR   CTD; 406484; -.
DR   ZFIN; ZDB-GENE-040426-2281; leg1.2.
DR   eggNOG; ENOG502R60Y; Eukaryota.
DR   InParanoid; Q4QRF7; -.
DR   OrthoDB; 1401349at2759; -.
DR   PRO; PR:Q4QRF7; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IDA:ZFIN.
DR   GO; GO:0055123; P:digestive system development; IMP:ZFIN.
DR   GO; GO:0001889; P:liver development; IMP:ZFIN.
DR   InterPro; IPR008499; Leg1.
DR   PANTHER; PTHR18820; PTHR18820; 1.
DR   Pfam; PF05612; Leg1; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..364
FT                   /note="Protein leg1b"
FT                   /id="PRO_0000252387"
FT   CARBOHYD        70
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        198
FT                   /note="Q -> H (in Ref. 1; AAH65450)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        204
FT                   /note="E -> D (in Ref. 1; AAH65450)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        269
FT                   /note="S -> A (in Ref. 1; AAH65450)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   364 AA;  39839 MW;  91A2E4833435314B CRC64;
     MSEMGFLRSV AAVLLLAVFS HAAVVTENGL PIQWRKAPSD LSHLPISNTG VQVNPWVYSQ
     RMTMLKMVIN ATNAYMSSMG PGEQENPLWS LPLQLGWKLK SGRLADPTPD SSSTCGSEAS
     DPVCISPLSF FGCVNYYLSV LPFLAAVETG VVSSGGHQML IQVPAEVAQD YCSSYSDCST
     KHPNTMAKWH LFFQSLRQVS QSKESDFNKK DSILGLMWAA EEESIQTASG ACTERQKLYS
     SPEVSFQQSS VNLGAFISAA HYHASIERSG KFMSHLPSRV LQEADSPPNI ADLSTEENHA
     LYMLSWMNSI NQLLGGSLVD LWRKAMCSAQ AREKGQAFLD DLILDPKFPG SSLWSIICVM
     STSC