LEG1H_HUMAN
ID LEG1H_HUMAN Reviewed; 330 AA.
AC Q6P5S2; B4E1I0; Q5VUP2;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein LEG1 homolog {ECO:0000250|UniProtKB:A5PF61, ECO:0000250|UniProtKB:Q4QRF7};
DE Flags: Precursor;
GN Name=LEG1 {ECO:0000250|UniProtKB:A5PF61, ECO:0000250|UniProtKB:Q4QRF7};
GN Synonyms=C6orf58 {ECO:0000312|HGNC:HGNC:20960};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-69, AND TISSUE SPECIFICITY.
RC TISSUE=Saliva;
RX PubMed=16740002; DOI=10.1021/pr050492k;
RA Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.;
RT "Identification of N-linked glycoproteins in human saliva by glycoprotein
RT capture and mass spectrometry.";
RL J. Proteome Res. 5:1493-1503(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND TISSUE SPECIFICITY.
RX PubMed=20651090; DOI=10.1177/0022034510375824;
RA Mangum J.E., Crombie F.A., Kilpatrick N., Manton D.J., Hubbard M.J.;
RT "Surface integrity governs the proteome of hypomineralized enamel.";
RL J. Dent. Res. 89:1160-1165(2010).
CC -!- FUNCTION: May be involved in early liver development.
CC {ECO:0000250|UniProtKB:A5PF61, ECO:0000250|UniProtKB:Q4QRF7}.
CC -!- INTERACTION:
CC Q6P5S2; P04083: ANXA1; NbExp=3; IntAct=EBI-11750531, EBI-354007;
CC Q6P5S2; P27797: CALR; NbExp=3; IntAct=EBI-11750531, EBI-1049597;
CC Q6P5S2; P36957: DLST; NbExp=3; IntAct=EBI-11750531, EBI-351007;
CC Q6P5S2; Q8TDX7: NEK7; NbExp=3; IntAct=EBI-11750531, EBI-1055945;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:A5PF61,
CC ECO:0000250|UniProtKB:Q4QRF7}.
CC -!- TISSUE SPECIFICITY: Detected in saliva and in hypomineralized dental
CC enamel (at protein level). {ECO:0000269|PubMed:16740002,
CC ECO:0000269|PubMed:20651090}.
CC -!- SIMILARITY: Belongs to the LEG1 family. {ECO:0000305}.
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DR EMBL; AK303850; BAG64792.1; -; mRNA.
DR EMBL; AL583806; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471051; EAW48094.1; -; Genomic_DNA.
DR EMBL; BC062712; AAH62712.2; -; mRNA.
DR CCDS; CCDS34533.1; -.
DR RefSeq; NP_001010905.1; NM_001010905.2.
DR AlphaFoldDB; Q6P5S2; -.
DR SMR; Q6P5S2; -.
DR BioGRID; 131603; 24.
DR IntAct; Q6P5S2; 8.
DR STRING; 9606.ENSP00000328069; -.
DR GlyGen; Q6P5S2; 3 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q6P5S2; -.
DR PhosphoSitePlus; Q6P5S2; -.
DR BioMuta; C6orf58; -.
DR DMDM; 74749125; -.
DR MassIVE; Q6P5S2; -.
DR PaxDb; Q6P5S2; -.
DR PeptideAtlas; Q6P5S2; -.
DR PRIDE; Q6P5S2; -.
DR ProteomicsDB; 67003; -.
DR Antibodypedia; 49859; 164 antibodies from 17 providers.
DR DNASU; 352999; -.
DR Ensembl; ENST00000329722.8; ENSP00000328069.7; ENSG00000184530.9.
DR GeneID; 352999; -.
DR KEGG; hsa:352999; -.
DR MANE-Select; ENST00000329722.8; ENSP00000328069.7; NM_001010905.3; NP_001010905.1.
DR UCSC; uc003qbh.5; human.
DR CTD; 352999; -.
DR DisGeNET; 352999; -.
DR GeneCards; C6orf58; -.
DR HGNC; HGNC:20960; C6orf58.
DR HPA; ENSG00000184530; Group enriched (intestine, salivary gland).
DR neXtProt; NX_Q6P5S2; -.
DR OpenTargets; ENSG00000184530; -.
DR PharmGKB; PA134881952; -.
DR VEuPathDB; HostDB:ENSG00000184530; -.
DR eggNOG; ENOG502QVPP; Eukaryota.
DR GeneTree; ENSGT00390000004904; -.
DR HOGENOM; CLU_071068_0_0_1; -.
DR InParanoid; Q6P5S2; -.
DR OMA; DIEPPLW; -.
DR OrthoDB; 1401349at2759; -.
DR PhylomeDB; Q6P5S2; -.
DR TreeFam; TF332991; -.
DR PathwayCommons; Q6P5S2; -.
DR SignaLink; Q6P5S2; -.
DR BioGRID-ORCS; 352999; 3 hits in 1051 CRISPR screens.
DR ChiTaRS; C6orf58; human.
DR GeneWiki; C6orf58; -.
DR GenomeRNAi; 352999; -.
DR Pharos; Q6P5S2; Tbio.
DR PRO; PR:Q6P5S2; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q6P5S2; protein.
DR Bgee; ENSG00000184530; Expressed in trachea and 102 other tissues.
DR Genevisible; Q6P5S2; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR InterPro; IPR008499; Leg1.
DR PANTHER; PTHR18820; PTHR18820; 1.
DR Pfam; PF05612; Leg1; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..330
FT /note="Protein LEG1 homolog"
FT /id="PRO_0000252385"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 69
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:16740002"
FT VARIANT 308
FT /note="C -> G (in dbSNP:rs9491833)"
FT /id="VAR_033675"
SQ SEQUENCE 330 AA; 37926 MW; 75F8219FC2C76914 CRC64;
MAFLPSWVCV LVGSFSASLA GTSNLSETEP PLWKESPGQL SDYRVENSMY IINPWVYLER
MGMYKIILNQ TARYFAKFAP DNEQNILWGL PLQYGWQYRT GRLADPTRRT NCGYESGDHM
CISVDSWWAD LNYFLSSLPF LAAVDSGVMG ISSDQVRLLP PPKNERKFCY DVSSCRSSFP
ETMNKWNTFY QYLQSPFSKF DDLLKYLWAA HTSTLADNIK SFEDRYDYYS KAEAHFERSW
VLAVDHLAAV LFPTTLIRSY KFQKGMPPRI LLNTDVAPFI SDFTAFQNVV LVLLNMLDNV
DKSIGYLCTE KSNVYRDHSE SSSRSYGNNS
