LEG4_CHICK
ID LEG4_CHICK Reviewed; 135 AA.
AC P07583;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Beta-galactoside-binding lectin;
DE AltName: Full=14 kDa lectin;
DE AltName: Full=C-14;
DE AltName: Full=Galectin CG-1B;
GN Name=CG-1B;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3004444; DOI=10.1016/0006-291x(86)90525-5;
RA Ohyama Y., Hirabayashi J., Oda Y., Ohno S., Kawasaki H., Suzuki K.,
RA Kasai K.;
RT "Nucleotide sequence of chick 14K beta-galactoside-binding lectin mRNA.";
RL Biochem. Biophys. Res. Commun. 134:51-56(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3182759; DOI=10.1093/oxfordjournals.jbchem.a122436;
RA Ohyama Y., Kasai K.;
RT "Isolation and characterization of the chick 14K beta-galactoside-binding
RT lectin gene.";
RL J. Biochem. 104:173-177(1988).
RN [3]
RP PROTEIN SEQUENCE OF 2-135, AND ACETYLATION AT SER-2.
RC TISSUE=Embryo;
RX PubMed=3597352; DOI=10.1093/jb/101.3.775;
RA Hirabayashi J., Kawasaki H., Suzuki K., Kasai K.;
RT "Complete amino acid sequence of 14 kDa beta-galactoside-binding lectin of
RT chick embryo.";
RL J. Biochem. 101:775-787(1987).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBUNIT, MUTAGENESIS OF CYS-8, AND
RP DISULFIDE BOND.
RC STRAIN=Bantam;
RX PubMed=18848566; DOI=10.1016/j.jmb.2008.09.054;
RA Lopez-Lucendo M.F., Solis D., Saiz J.L., Kaltner H., Russwurm R., Andre S.,
RA Gabius H.J., Romero A.;
RT "Homodimeric chicken galectin CG-1B (C-14): Crystal structure and detection
RT of unique redox-dependent shape changes involving inter- and intrasubunit
RT disulfide bridges by gel filtration, ultracentrifugation, site-directed
RT mutagenesis, and peptide mass fingerprinting.";
RL J. Mol. Biol. 386:366-378(2009).
RN [5]
RP FUNCTION, AND INTERACTION WITH NEWCASTLE DISEASE VIRUS PROTEIN HN
RP (MICROBIAL INFECTION).
RX PubMed=28978647; DOI=10.1074/jbc.m116.772897;
RA Sun J., Han Z., Qi T., Zhao R., Liu S.;
RT "Chicken galectin-1B inhibits Newcastle disease virus adsorption and
RT replication through binding to hemagglutinin-neuraminidase (HN)
RT glycoprotein.";
RL J. Biol. Chem. 292:20141-20161(2017).
CC -!- FUNCTION: This protein binds beta-galactoside. May participate in host
CC antiviral defense through specific interaction with glycans on the
CC viral envelope glycoproteins. {ECO:0000269|PubMed:28978647}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000269|PubMed:18848566}.
CC -!- SUBUNIT: (Microbial infection) Interacts with newcastle disease virus
CC protein HN; this interaction inhibits viral adsorption rather than
CC internalization. {ECO:0000269|PubMed:28978647}.
CC -!- TISSUE SPECIFICITY: Mainly in the intestine (adult), mainly in the skin
CC (embryo).
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DR EMBL; M11674; AAA48779.1; -; mRNA.
DR EMBL; D00311; BAA00214.1; -; Genomic_DNA.
DR PIR; JX0042; LNCH14.
DR RefSeq; NP_990826.1; NM_205495.1.
DR PDB; 3DUI; X-ray; 2.10 A; A/B=1-135.
DR PDBsum; 3DUI; -.
DR AlphaFoldDB; P07583; -.
DR SMR; P07583; -.
DR STRING; 9031.ENSGALP00000020275; -.
DR BindingDB; P07583; -.
DR ChEMBL; CHEMBL2189155; -.
DR UniLectin; P07583; -.
DR iPTMnet; P07583; -.
DR PaxDb; P07583; -.
DR Ensembl; ENSGALT00000020302; ENSGALP00000020275; ENSGALG00000012420.
DR GeneID; 396491; -.
DR KEGG; gga:396491; -.
DR CTD; 396491; -.
DR VEuPathDB; HostDB:geneid_396491; -.
DR eggNOG; KOG3587; Eukaryota.
DR GeneTree; ENSGT00940000155534; -.
DR HOGENOM; CLU_037794_5_0_1; -.
DR InParanoid; P07583; -.
DR OMA; CNSKKME; -.
DR OrthoDB; 1429018at2759; -.
DR PhylomeDB; P07583; -.
DR TreeFam; TF315551; -.
DR Reactome; R-GGA-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-GGA-8957275; Post-translational protein phosphorylation.
DR EvolutionaryTrace; P07583; -.
DR PRO; PR:P07583; -.
DR Proteomes; UP000000539; Chromosome 1.
DR Bgee; ENSGALG00000012420; Expressed in lung and 13 other tissues.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IBA:GO_Central.
DR GO; GO:0043236; F:laminin binding; IBA:GO_Central.
DR CDD; cd00070; GLECT; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR044156; Galectin-like.
DR InterPro; IPR001079; Galectin_CRD.
DR PANTHER; PTHR11346; PTHR11346; 1.
DR Pfam; PF00337; Gal-bind_lectin; 1.
DR SMART; SM00908; Gal-bind_lectin; 1.
DR SMART; SM00276; GLECT; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS51304; GALECTIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; Disulfide bond;
KW Lectin; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:3597352"
FT CHAIN 2..135
FT /note="Beta-galactoside-binding lectin"
FT /id="PRO_0000076951"
FT DOMAIN 5..135
FT /note="Galectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00639"
FT BINDING 46..50
FT /ligand="a beta-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:28034"
FT /evidence="ECO:0000250"
FT BINDING 54
FT /ligand="a beta-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:28034"
FT /evidence="ECO:0000250"
FT BINDING 63
FT /ligand="a beta-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:28034"
FT /evidence="ECO:0000250"
FT BINDING 70..76
FT /ligand="a beta-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:28034"
FT /evidence="ECO:0000255"
FT BINDING 70..73
FT /ligand="a beta-D-galactoside"
FT /ligand_id="ChEBI:CHEBI:28034"
FT /evidence="ECO:0000250"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000269|PubMed:3597352"
FT DISULFID 3..8
FT /note="Redox-active"
FT /evidence="ECO:0000269|PubMed:18848566"
FT DISULFID 8
FT /note="Interchain; in linked form"
FT /evidence="ECO:0000269|PubMed:18848566"
FT MUTAGEN 8
FT /note="C->V: No redox-dependent shape changes."
FT /evidence="ECO:0000269|PubMed:18848566"
FT STRAND 7..9
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 18..25
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 31..39
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 42..53
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 56..68
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 86..93
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 95..102
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 105..110
FT /evidence="ECO:0007829|PDB:3DUI"
FT STRAND 120..135
FT /evidence="ECO:0007829|PDB:3DUI"
SQ SEQUENCE 135 AA; 15063 MW; 5059E30CC51F899A CRC64;
MSCQGPVCTN LGLKPGQRLT VKGIIAPNAK SFVMNLGKDS THLGLHFNPR FDAHGDVNLI
VCNSKKMEEW GTEQRETVFP FQKGAPIEIT FSINPSDLTV HLPGHQFSFP NRLGLSVFDY
FDTHGDFTLR SVSWE
