ID   LEGA_GOSHI              Reviewed;         509 AA.
AC   P09802; Q39790;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Legumin A;
DE   AltName: Full=Beta-globulin;
DE   AltName: Full=LEGA-C94;
DE   Contains:
DE     RecName: Full=Legumin A acidic chain;
DE   Contains:
DE     RecName: Full=Legumin A basic chain;
DE   Flags: Precursor;
GN   Name=LEGA;
OS   Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX   NCBI_TaxID=3635;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   PubMed=16668521; DOI=10.1104/pp.97.3.1268;
RA   Galau G.A., Wang H.Y., Hughes D.W.;
RT   "Sequence of the Gossypium hirsutum D-genome alloallele of Legumin A and
RT   its mRNA.";
RL   Plant Physiol. 97:1268-1270(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 3-509.
RX   AGRICOLA=IND87019922; DOI=10.1007/BF00020331;
RA   Chlan C.A., Pyle J.B., Legocki A.B., Dure L. III;
RT   "Developmental biochemistry of cottonseed embryogenesis and germination.
RT   XVIII. cDNA and amino acid sequences of the members of the storage protein
RT   families.";
RL   Plant Mol. Biol. 7:475-489(1986).
CC   -!- FUNCTION: This is a seed storage protein.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; M69188; AAA33053.1; -; Genomic_DNA.
DR   EMBL; M73072; AAA33065.1; -; mRNA.
DR   EMBL; M16905; AAA33072.1; -; mRNA.
DR   PIR; B30838; FWCNBA.
DR   RefSeq; XP_016701249.1; XM_016845760.1.
DR   AlphaFoldDB; P09802; -.
DR   SMR; P09802; -.
DR   STRING; 3635.P09802; -.
DR   PRIDE; P09802; -.
DR   GeneID; 107916455; -.
DR   KEGG; ghi:107916455; -.
DR   OMA; KFGRGQE; -.
DR   Proteomes; UP000189702; Chromosome 3.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   GO; GO:0010431; P:seed maturation; IEA:UniProt.
DR   Gene3D; 2.60.120.10; -; 3.
DR   InterPro; IPR022379; 11S_seedstore_CS.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Reference proteome; Seed storage protein; Signal;
KW   Storage protein.
FT   SIGNAL          1..21
FT   CHAIN           22..509
FT                   /note="Legumin A"
FT                   /id="PRO_0000032060"
FT   CHAIN           22..324
FT                   /note="Legumin A acidic chain"
FT                   /id="PRO_0000032061"
FT   CHAIN           325..509
FT                   /note="Legumin A basic chain"
FT                   /id="PRO_0000032062"
FT   DOMAIN          39..273
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          337..486
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          187..248
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          298..325
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        213..227
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        228..245
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        298..322
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        34..67
FT                   /evidence="ECO:0000250"
FT   DISULFID        110..331
FT                   /note="Interchain (between acidic and basic chains)"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        3
FT                   /note="I -> V (in Ref. 2; AAA33072)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   509 AA;  58425 MW;  E9235CCC9F946F37 CRC64;
     MAINPSLLFL SLLFLFNGCL ARQTFSSQQS QNECQINRLR ASAPQTRIRS EAGTTEWWNP
     NCQQLRCAGV SVMRQTIEPN GLVLPSFTNA PQLLYIVQGR GIQGIVMPGC AETFQDSQQW
     QHQSRGRFQD QHQKVRRFRQ GDIIALPQGV VHWSYNDGNE RVVTINLLDT GNSANQLDNI
     PRRFHLAGNP EEEQRQLRRL AQQMQGRSER GEESEEEEGE GEEEEEEDNP SRRSRHQEEE
     EQGRESSSCN NLLCAFDRNF LAQAFNVDHD IIRKIQRVRG NRGTIIRVRD RLQVVTPPRM
     EEEEREERQQ EQRYRHTRGG SQDNGLEETF CSMRIKENLA DPERADIFNP QAGRISTLNR
     FNLPILQRLE LSAERGVLYN RAGLIPQWNV NAHKILYMLR GCARVQVVNH NGDAVFDDNV
     EQGQLLTVPQ NFAFMKQAGN EGAEWISFFT NSEATNTPMA GSVSFMRALP EEVVAASYQV
     SREDARRIKF NNKNTFFFTP SQSERRADA