LEGA_PEA
ID LEGA_PEA Reviewed; 517 AA.
AC P02857; Q41033; Q41034;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Legumin A;
DE Contains:
DE RecName: Full=Legumin A alpha chain;
DE AltName: Full=Legumin A acidic chain;
DE Contains:
DE RecName: Full=Legumin A beta chain;
DE AltName: Full=Legumin A basic chain;
DE Flags: Precursor;
GN Name=LEGA;
OS Pisum sativum (Garden pea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Feltham First;
RX PubMed=6330672; DOI=10.1093/nar/12.11.4493;
RA Lycett G.W., Croy R.R.D., Shirsat A.H., Boulter D.;
RT "The complete nucleotide sequence of a legumin gene from pea (Pisum sativum
RT L.).";
RL Nucleic Acids Res. 12:4493-4506(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE OF 209-411.
RX AGRICOLA=IND87019921; DOI=10.1007/BF00020330;
RA Domoney C., Barker D., Casey R.;
RT "The complete deduced amino acid sequences of legumin beta-polypeptides
RT from different genetic loci in Pisum.";
RL Plant Mol. Biol. 7:467-474(1986).
CC -!- FUNCTION: This protein found in the seeds of many leguminous and non-
CC leguminous plants is the source of sulfur-containing amino acids in
CC seed meals.
CC -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC chain derived from a single precursor and linked by a disulfide bond.
CC -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA26720.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X02982; CAA26720.1; ALT_SEQ; Genomic_DNA.
DR EMBL; M16904; AAA33677.1; -; mRNA.
DR PIR; A22866; FWPMLA.
DR PIR; S09559; S09559.
DR PIR; T06452; T06452.
DR PDB; 3KSC; X-ray; 2.61 A; A/B/C/D/E/F=22-517.
DR PDBsum; 3KSC; -.
DR AlphaFoldDB; P02857; -.
DR SMR; P02857; -.
DR PRIDE; P02857; -.
DR EvolutionaryTrace; P02857; -.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR022379; 11S_seedstore_CS.
DR InterPro; IPR006044; 11S_seedstore_pln.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 2.
DR PRINTS; PR00439; 11SGLOBULIN.
DR SMART; SM00835; Cupin_1; 2.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Seed storage protein; Signal;
KW Storage protein.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..332
FT /note="Legumin A alpha chain"
FT /id="PRO_0000032066"
FT CHAIN 333..517
FT /note="Legumin A beta chain"
FT /id="PRO_0000032067"
FT DOMAIN 36..232
FT /note="Cupin type-1 1"
FT /evidence="ECO:0000255"
FT DOMAIN 345..494
FT /note="Cupin type-1 2"
FT /evidence="ECO:0000255"
FT REGION 249..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 263..290
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..332
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 31..64
FT /evidence="ECO:0000250"
FT DISULFID 107..339
FT /note="Interchain (between alpha and beta chains)"
FT /evidence="ECO:0000255"
FT CONFLICT 219
FT /note="Y -> F (in Ref. 2; AAA33677)"
FT /evidence="ECO:0000305"
FT CONFLICT 319
FT /note="G -> R (in Ref. 2; AAA33677)"
FT /evidence="ECO:0000305"
FT STRAND 42..47
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 50..54
FT /evidence="ECO:0007829|PDB:3KSC"
FT HELIX 60..65
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 68..74
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 78..87
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 89..95
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 98..103
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 132..135
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 139..143
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 148..153
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 155..157
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 159..165
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 180..186
FT /evidence="ECO:0007829|PDB:3KSC"
FT HELIX 192..194
FT /evidence="ECO:0007829|PDB:3KSC"
FT HELIX 211..214
FT /evidence="ECO:0007829|PDB:3KSC"
FT HELIX 217..224
FT /evidence="ECO:0007829|PDB:3KSC"
FT HELIX 228..234
FT /evidence="ECO:0007829|PDB:3KSC"
FT TURN 235..238
FT /evidence="ECO:0007829|PDB:3KSC"
FT TURN 241..243
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 245..248
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 339..341
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 344..346
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 354..357
FT /evidence="ECO:0007829|PDB:3KSC"
FT TURN 358..360
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 361..366
FT /evidence="ECO:0007829|PDB:3KSC"
FT TURN 368..370
FT /evidence="ECO:0007829|PDB:3KSC"
FT HELIX 374..377
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 380..387
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 391..399
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 402..416
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 422..429
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 433..436
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 441..446
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 448..459
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 464..469
FT /evidence="ECO:0007829|PDB:3KSC"
FT TURN 473..476
FT /evidence="ECO:0007829|PDB:3KSC"
FT HELIX 479..486
FT /evidence="ECO:0007829|PDB:3KSC"
FT HELIX 490..498
FT /evidence="ECO:0007829|PDB:3KSC"
FT STRAND 503..506
FT /evidence="ECO:0007829|PDB:3KSC"
SQ SEQUENCE 517 AA; 58805 MW; CD2CA2B41758D0BA CRC64;
MAKLLALSLS FCFLLLGGCF ALREQPQQNE CQLERLDALE PDNRIESEGG LIETWNPNNK
QFRCAGVALS RATLQRNALR RPYYSNAPQE IFIQQGNGYF GMVFPGCPET FEEPQESEQG
EGRRYRDRHQ KVNRFREGDI IAVPTGIVFW MYNDQDTPVI AVSLTDIRSS NNQLDQMPRR
FYLAGNHEQE FLQYQHQQGG KQEQENEGNN IFSGFKRDYL EDAFNVNRHI VDRLQGRNED
EEKGAIVKVK GGLSIISPPE KQARHQRGSR QEEDEDEEKQ PRHQRGSRQE EEEDEDEERQ
PRHQRRRGEE EEEDKKERGG SQKGKSRRQG DNGLEETVCT AKLRLNIGPS SSPDIYNPEA
GRIKTVTSLD LPVLRWLKLS AEHGSLHKNA MFVPHYNLNA NSIIYALKGR ARLQVVNCNG
NTVFDGELEA GRALTVPQNY AVAAKSLSDR FSYVAFKTND RAGIARLAGT SSVINNLPLD
VVAATFNLQR NEARQLKSNN PFKFLVPARE SENRASA
