ID   LEGB_GOSHI              Reviewed;         516 AA.
AC   P09800;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Legumin B;
DE   AltName: Full=Beta-globulin B;
DE   AltName: Full=LEGB-C134;
DE   Contains:
DE     RecName: Full=Legumin B acidic chain;
DE   Contains:
DE     RecName: Full=Legumin B basic chain;
DE   Flags: Precursor;
GN   Name=LEGB;
OS   Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX   NCBI_TaxID=3635;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   AGRICOLA=IND87019922; DOI=10.1007/BF00020331;
RA   Chlan C.A., Pyle J.B., Legocki A.B., Dure L. III;
RT   "Developmental biochemistry of cottonseed embryogenesis and germination.
RT   XVIII. cDNA and amino acid sequences of the members of the storage protein
RT   families.";
RL   Plant Mol. Biol. 7:475-489(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Stoneville 887;
RA   Chlan C.A.;
RT   "Structural similarities between the legumins of Gossypium hirsutum:
RT   sequence of the Legumin B gene1.";
RL   (er) Plant Gene Register PGR95-141(1995).
CC   -!- FUNCTION: This is a seed storage protein.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; M16936; AAA33070.1; -; mRNA.
DR   EMBL; U43727; AAD09844.1; -; Genomic_DNA.
DR   PIR; C30838; FWCNBB.
DR   RefSeq; XP_016699481.1; XM_016843992.1.
DR   AlphaFoldDB; P09800; -.
DR   SMR; P09800; -.
DR   STRING; 3635.P09800; -.
DR   PRIDE; P09800; -.
DR   GeneID; 107914926; -.
DR   KEGG; ghi:107914926; -.
DR   OMA; FCTMKLR; -.
DR   Proteomes; UP000189702; Chromosome 22.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   GO; GO:0010431; P:seed maturation; IEA:UniProt.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR022379; 11S_seedstore_CS.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Reference proteome; Seed storage protein; Signal;
KW   Storage protein.
FT   SIGNAL          1..22
FT   CHAIN           23..516
FT                   /note="Legumin B"
FT                   /id="PRO_0000032063"
FT   CHAIN           23..335
FT                   /note="Legumin B acidic chain"
FT                   /id="PRO_0000032064"
FT   CHAIN           336..516
FT                   /note="Legumin B basic chain"
FT                   /id="PRO_0000032065"
FT   DOMAIN          55..264
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          348..496
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          207..245
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          286..342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        217..237
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        286..335
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        50..83
FT                   /evidence="ECO:0000250"
FT   DISULFID        126..342
FT                   /note="Interchain (between acidic and basic chains)"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   516 AA;  58709 MW;  3AA532E0873897FD CRC64;
     MAYTSLLSFS VCLLVLFHGC CAQIDLVTNH HQDPPWGQPQ QPQPRHQSQC QLQNLNALQP
     KHRFRSEAGE TEFWDQNEDQ FQCAGVAFLR HKIQRKGLLL PSFTSAPMLF YVEQGEGIHG
     AVFPGCPETY QSQSQQNIQD RPQRDQHQKL RRLKEGDVVA LPAGVAHWIF NNGRSQLVLV
     ALVDVGNDAN QLDENFRKFF LAGSPQGGVV RGGQSRDRNQ RQSRTQRGER EEEESQESGG
     NNVLSGFRDN LLAQAFGIDT RLARKLQNER DNRGAIVRME HGFEWPEEGQ RRQGREEEGE
     EEREPKWQRR QESQEEGSEE EEREERGRGR RRSGNGLEET FCSMRLKHRT PASSADVFNP
     RGGRITTVNS FNLPILQYLQ LSAERGVLYN NAIYAPHWNM NAHSIVYITR GNGRIQIVSE
     NGEAIFDEQV ERGQVITVPQ NHAVVKKAGR RGFEWIAFKT NANAKISQIA GRVSIMRGLP
     VDVLANSFGI SREEAMRLKH NRQEVSVFSP RQGSQQ