ID   LEGJ_PEA                Reviewed;         503 AA.
AC   P05692;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Legumin J;
DE   Contains:
DE     RecName: Full=Legumin J alpha chain;
DE     AltName: Full=Legumin J acidic chain;
DE   Contains:
DE     RecName: Full=Legumin J beta chain;
DE     AltName: Full=Legumin J basic chain;
DE   Flags: Precursor;
GN   Name=LEGJ;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=cv. Feltham First;
RX   PubMed=3355508; DOI=10.1042/bj2500015;
RA   Gatehouse J.A., Bown D., Gilroy J., Levasseur M., Castleton J.,
RA   Ellis T.H.N.;
RT   "Two genes encoding 'minor' legumin polypeptides in pea (Pisum sativum L.).
RT   Characterization and complete sequence of the LegJ gene.";
RL   Biochem. J. 250:15-24(1988).
CC   -!- FUNCTION: This protein found in the seeds of many leguminous and non-
CC       leguminous plants is the source of sulfur-containing amino acids in
CC       seed meals.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; X07014; CAA30067.1; -; Genomic_DNA.
DR   PIR; S00336; S00336.
DR   AlphaFoldDB; P05692; -.
DR   SMR; P05692; -.
DR   PRIDE; P05692; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR022379; 11S_seedstore_CS.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Seed storage protein; Signal;
KW   Storage protein.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..322
FT                   /note="Legumin J alpha chain"
FT                   /id="PRO_0000032072"
FT   CHAIN           323..503
FT                   /note="Legumin J beta chain"
FT                   /id="PRO_0000032073"
FT   DOMAIN          38..257
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          335..482
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          111..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          185..235
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          253..323
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        253..301
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        33..66
FT                   /evidence="ECO:0000250"
FT   DISULFID        109..329
FT                   /note="Interchain (between alpha and beta chains)"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   503 AA;  56895 MW;  3F90F4DE85046DAA CRC64;
     MSKPFLSLLS LSLLLFASAC LATSSEFDRL NQCQLDSINA LEPDHRVESE AGLTETWNPN
     HPELKCAGVS LIRRTIDPNG LHLPSFSPSP QLIFIIQGKG VLGLSFPGCP ETYEEPRSSQ
     SRQESRQQQG DSHQKVRRFR KGDIIAIPSG IPYWTYNHGD EPLVAISLLD TSNIANQLDS
     TPRVFYLGGN PETEFPETQE EQQGRHRQKH SYPVGRRSGH HQQEEESEEQ NEGNSVLSGF
     SSEFLAQTFN TEEDTAKRLR SPRDERSQIV RVEGGLRIIK PKGKEEEEKE QSHSHSHREE
     KEEEEEEEED EEEKQRSEER KNGLEETICS AKIRENIADA ARADLYNPRA GRISTANSLT
     LPVLRYLRLS AEYVRLYRNG IYAPHWNINA NSLLYVIRGE GRVRIVNCQG NTVFDNKVRK
     GQLVVVPQNF VVAEQAGEEE GLEYVVFKTN DRAAVSHVQQ VFRATPSEVL ANAFGLRQRQ
     VTELKLSGNR GPLVHPRSQS QSH