LEO1_CAEEL
ID LEO1_CAEEL Reviewed; 430 AA.
AC Q17431;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=RNA polymerase-associated protein LEO1 {ECO:0000250|UniProtKB:P38439};
GN Name=leo-1 {ECO:0000312|WormBase:B0035.11};
GN ORFNames=B0035.11 {ECO:0000312|WormBase:B0035.11};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=24721716; DOI=10.1016/j.ydbio.2014.04.002;
RA Kubota Y., Tsuyama K., Takabayashi Y., Haruta N., Maruyama R., Iida N.,
RA Sugimoto A.;
RT "The PAF1 complex is involved in embryonic epidermal morphogenesis in
RT Caenorhabditis elegans.";
RL Dev. Biol. 391:43-53(2014).
CC -!- FUNCTION: Component of the PAF1 complex which is a multifunctional
CC complex involved in transcription initiation via genetic interactions
CC with TATA-binding proteins, elongation and transcription-coupled
CC histone modification. {ECO:0000250|UniProtKB:P38439}.
CC -!- SUBUNIT: Component of the PAF1 complex which consists of at least cdc-
CC 73, ctr-9, leo-1, pafo-1 and rtfo-1. {ECO:0000250|UniProtKB:P38439}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24721716}. Cytoplasm
CC {ECO:0000269|PubMed:24721716}. Note=Nuclear localization depends on
CC ctr-9, pafo-1 and cdc-73. Located in nuclei before mitosis. Located in
CC the cytoplasm upon mitotic nuclear membrane breakdown. Located in
CC nuclei when nuclear envelope is reassembled in telophase.
CC {ECO:0000269|PubMed:24721716, ECO:0000303|PubMed:24721716}.
CC -!- DEVELOPMENTAL STAGE: Expressed in both somatic cells and germ cells
CC from the one-cell stage onwards. {ECO:0000269|PubMed:24721716}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knock-down is maternal effect
CC embryonic lethal. Embryogenesis proceeds more slowly, with embryos
CC displaying defects in the positioning and shape of epidermal cells.
CC {ECO:0000269|PubMed:24721716}.
CC -!- SIMILARITY: Belongs to the LEO1 family. {ECO:0000255}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z73102; CAA97406.1; -; Genomic_DNA.
DR PIR; T18651; T18651.
DR RefSeq; NP_502135.1; NM_069734.3.
DR AlphaFoldDB; Q17431; -.
DR SMR; Q17431; -.
DR ComplexPortal; CPX-966; PAF1 complex.
DR STRING; 6239.B0035.11; -.
DR EPD; Q17431; -.
DR PaxDb; Q17431; -.
DR PeptideAtlas; Q17431; -.
DR EnsemblMetazoa; B0035.11.1; B0035.11.1; WBGene00007110.
DR GeneID; 178052; -.
DR KEGG; cel:CELE_B0035.11; -.
DR UCSC; B0035.11; c. elegans.
DR CTD; 178052; -.
DR WormBase; B0035.11; CE05166; WBGene00007110; leo-1.
DR eggNOG; KOG2428; Eukaryota.
DR GeneTree; ENSGT00550000074952; -.
DR HOGENOM; CLU_638168_0_0_1; -.
DR InParanoid; Q17431; -.
DR OMA; ARICPKF; -.
DR OrthoDB; 1036192at2759; -.
DR PhylomeDB; Q17431; -.
DR Reactome; R-CEL-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-CEL-201722; Formation of the beta-catenin:TCF transactivating complex.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-75955; RNA Polymerase II Transcription Elongation.
DR PRO; PR:Q17431; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00007110; Expressed in embryo and 4 other tissues.
DR GO; GO:0016593; C:Cdc73/Paf1 complex; IC:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:1990269; F:RNA polymerase II C-terminal domain phosphoserine binding; IBA:GO_Central.
DR GO; GO:0016571; P:histone methylation; IC:ComplexPortal.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR InterPro; IPR007149; Leo1.
DR PANTHER; PTHR23146; PTHR23146; 1.
DR Pfam; PF04004; Leo1; 1.
PE 2: Evidence at transcript level;
KW Activator; Coiled coil; Cytoplasm; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..430
FT /note="RNA polymerase-associated protein LEO1"
FT /id="PRO_0000431510"
FT REGION 1..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 349..430
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 326..347
FT /evidence="ECO:0000255"
FT COILED 409..429
FT /evidence="ECO:0000255"
FT COMPBIAS 1..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..72
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..118
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..430
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 430 AA; 48063 MW; B6753387A0FC1F84 CRC64;
MSSSEGNSDA SAPGTPVKSS TPSSRGSSPD SPKKQEPSKK RAILNESDDD DDSRPAPRQL
SDSSDDNIHP RGGGEKSSPG AGGGLFGDLS GSSDDDSDRE GKPKESNTRA RLSDSDAESR
GSLNDLQGIV MANPDEIDED EKAKEHVHDT EMVTGRVTLE YAADPPHFVR MPNFLSVATH
PFDPQHYEED EDDEQAKLDD EGRTRLKLRV ENTLRWRVRK DENGKEIRES NAKIVKWDDG
TMSLYLGNEI FEVSLVPLNS NNLPHLYVKQ PTLMSAQAVL THRMTFRPHS TDSQTHRKVT
LNMADRSRKN AQVKVMDDVG QNPEITRREN ARKEEESLRA HIRRTQMVRN NFKVRGPRYA
GQYSDDEDMP TSSRKGKKKE APIIGASSES EDDHADTTKK SGSDSDSDEE YRKRKQQQKK
QIVTSDEESD
