ARF2B_SOLLC
ID ARF2B_SOLLC Reviewed; 828 AA.
AC K4DF01;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Auxin response factor 2B {ECO:0000303|PubMed:26716451, ECO:0000303|PubMed:26959229};
DE Short=SlARF2B {ECO:0000303|PubMed:26716451};
GN Name=ARF2B {ECO:0000303|PubMed:26716451, ECO:0000303|PubMed:26959229};
GN Synonyms=ARF11 {ECO:0000303|PubMed:21735233};
GN OrderedLocusNames=Solyc12g042070 {ECO:0000305|PubMed:26716451};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
RN [2]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND PHYLOGENETIC ANALYSIS.
RX PubMed=21735233; DOI=10.1007/s00299-011-1113-z;
RA Wu J., Wang F., Cheng L., Kong F., Peng Z., Liu S., Yu X., Lu G.;
RT "Identification, isolation and expression analysis of auxin response factor
RT (ARF) genes in Solanum lycopersicum.";
RL Plant Cell Rep. 30:2059-2073(2011).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=26716451; DOI=10.1371/journal.pgen.1005649;
RA Hao Y., Hu G., Breitel D., Liu M., Mila I., Frasse P., Fu Y., Aharoni A.,
RA Bouzayen M., Zouine M.;
RT "Auxin response factor SlARF2 is an essential component of the regulatory
RT mechanism controlling fruit ripening in tomato.";
RL PLoS Genet. 11:E1005649-E1005649(2015).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26959229; DOI=10.1371/journal.pgen.1005903;
RA Breitel D.A., Chappell-Maor L., Meir S., Panizel I., Puig C.P., Hao Y.,
RA Yifhar T., Yasuor H., Zouine M., Bouzayen M., Granell Richart A.,
RA Rogachev I., Aharoni A.;
RT "AUXIN RESPONSE FACTOR 2 intersects hormonal signals in the regulation of
RT tomato fruit ripening.";
RL PLoS Genet. 12:E1005903-E1005903(2016).
CC -!- FUNCTION: Auxin response factors (ARFs) are transcriptional factors
CC that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the
CC auxin-responsive promoter elements (AuxREs) (By similarity). Could act
CC as transcriptional activator or repressor. Involved in the control of
CC fruit ripening process. Regulates expression of a number of ripening
CC regulators, transcription factors, and ethylene biosynthesis and
CC signaling components (PubMed:26716451, PubMed:26959229). May act as a
CC transcriptional repressor of auxin-responsive genes (PubMed:26716451).
CC {ECO:0000255|RuleBase:RU004561, ECO:0000269|PubMed:26716451,
CC ECO:0000269|PubMed:26959229}.
CC -!- SUBUNIT: Homodimers and heterodimers. {ECO:0000255,
CC ECO:0000255|RuleBase:RU004561}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255, ECO:0000255|PROSITE-
CC ProRule:PRU00326, ECO:0000255|RuleBase:RU004561,
CC ECO:0000269|PubMed:26716451}.
CC -!- TISSUE SPECIFICITY: Expressed in root, leaf and stem (PubMed:26716451,
CC PubMed:21735233). Also expressed in flower and fruit (PubMed:26716451).
CC Expressed in flower buds about three days before opening including
CC stamen, petal and sepal with the highest in ovary (PubMed:21735233).
CC {ECO:0000269|PubMed:21735233, ECO:0000269|PubMed:26716451}.
CC -!- DEVELOPMENTAL STAGE: Expression increases during floral development.
CC Expressed during ovary development with the highest expression on the
CC third day after the flower fully opened. {ECO:0000269|PubMed:21735233}.
CC -!- INDUCTION: By tomato fruit ripening. Expression is up-regulated by
CC plant hormone auxin in tomato fruits. {ECO:0000269|PubMed:26716451}.
CC -!- DISRUPTION PHENOTYPE: Simultaneous RNAi-mediated silencing of both
CC ARF2A and ARF2B results in severe defects in tomato fruit ripening
CC process (PubMed:26959229, PubMed:26716451). Plants form triple
CC cotyledons and have enhanced root branching. Tomatoes have reduced
CC pigment accumulation, enhanced fruit firmness, low climacteric ethylene
CC production and inability to ripen upon exogenous application of
CC ethylene (PubMed:26716451). The fruits are either parthenocarpic or
CC contain only a few seeds, and the time from anthesis to breaker (Br)
CC developmental stage is significantly extended compared to wild type
CC fruit (PubMed:26959229). Altered expression of ethylene biosynthesis,
CC signaling and ethylene response factor (ERF) genes and genes involved
CC in the carotenoid pathway and ripening-related cell wall metabolism.
CC Up-regulates auxin-responsive genes (PubMed:26716451). Down-regulated
CC expression levels of ripening regulators, including RIN, AP2a, NOR,
CC TAGL1, ETR3, ERF1 and CNR at the red (R) fruit stage. Expression levels
CC of hormones such as abscisates, cytokinins and salicyclic acid are
CC altered, and levels of carotenoids phytoene, phytofluene and lycopene
CC are reduced in red fruits. Gibberellic acid (GA) and auxin expression
CC levels are unchanged. Compounds normally reduced upon ripening show
CC higher levels than wild type fruit as a result of simultaneous
CC silencing of ARF2A and ARF2B (PubMed:26959229).
CC {ECO:0000269|PubMed:26716451, ECO:0000269|PubMed:26959229}.
CC -!- SIMILARITY: Belongs to the ARF family. {ECO:0000255|RuleBase:RU004561}.
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DR EMBL; CM001075; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_004252281.1; XM_004252233.3.
DR AlphaFoldDB; K4DF01; -.
DR SMR; K4DF01; -.
DR STRING; 4081.Solyc12g042070.1.1; -.
DR PaxDb; K4DF01; -.
DR PRIDE; K4DF01; -.
DR GeneID; 101249712; -.
DR KEGG; sly:101249712; -.
DR eggNOG; ENOG502QRXI; Eukaryota.
DR HOGENOM; CLU_002626_2_2_1; -.
DR InParanoid; K4DF01; -.
DR OrthoDB; 214403at2759; -.
DR PhylomeDB; K4DF01; -.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; K4DF01; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd10017; B3_DNA; 1.
DR Gene3D; 2.40.330.10; -; 1.
DR InterPro; IPR010525; ARF_dom.
DR InterPro; IPR044835; ARF_plant.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31384; PTHR31384; 1.
DR Pfam; PF02309; AUX_IAA; 1.
DR Pfam; PF06507; Auxin_resp; 1.
DR Pfam; PF02362; B3; 1.
DR SMART; SM01019; B3; 1.
DR SUPFAM; SSF101936; SSF101936; 1.
DR PROSITE; PS50863; B3; 1.
DR PROSITE; PS51745; PB1; 1.
PE 2: Evidence at transcript level;
KW Auxin signaling pathway; Developmental protein; DNA-binding;
KW Fruit ripening; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..828
FT /note="Auxin response factor 2B"
FT /id="PRO_0000441019"
FT DOMAIN 703..786
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT DNA_BIND 128..230
FT /note="TF-B3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT REGION 348..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 681..703
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 791..828
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 377..397
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 681..700
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 805..828
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 828 AA; 92468 MW; F152847D499EF9EA CRC64;
MATSENCRNA AGAGKVDAEK ALYTELWRAC AGPLVTVPCE GELVFYFPQG HIEQVEASTN
QASDQQMPVY NLPSKILCRV INVLLKAEPD TDEVYAQVTL LPEPNQDENV VSKEPMPSPP
PRFHVHSFCK TLTASDTSTH GGFSVLRRHA DECLPPLDMS RQPPTQELVA KDLHANEWRF
RHIFRGQPRR HLLQSGWSVF VSSKRLVAGD AFIFLRGENG ELRVGVRRAM RQQGNAPSSV
ISSHSMHLGV LATAWHAIQT KTLFTVYYKP RTSPADFIVP YDQYMESLKN NYSIGMRFKM
RFEGEEAPEQ RFTGTIVGIE NADLKRWPES KWRCLKVRWD ETSAIPRPDR VSPWKVEPAL
SPPALNPLPI PRQKRPRSNV LPSSPDSSVL TREGSSKVVV DTSQASGFSR VLQGQEISTL
RGNFVENNES DSSEKPPIWQ PLLDDEKADV HSASRKCISD KRLPLGRPES SFTDLLSGFG
GQSSSSHGFH SPTGGQTAPA SWVKRQALDK ETDFSLLAKQ WSLVSSGLSL NLMESGLKGA
DTLYQMRGTS RLNCFNEYPT FPGHRPDNQQ GNWLMPPSVL PYIQMSAHSG EIMPKPMASP
QPEAMKPKEG NCKLFGIPLV SKCATIDPVM LRKNSPIHST SNMHFGIHPH QFPIIESDQR
SEQSKGSKLP DDGFIVHDQE EQFQTSHPGT RDREGKGLVH STRSCTKVHK QGTALGRSVD
LAKFNNYEEL IAELDHIFDF NGELKARNKN WLVVYTDDEG DMMLVGDDPW EFCGMVRKIF
IYTKDEVQRM NPGTLNSKGE DNSSVAEGSD AKEVKNLQLH IDSSPEDS
