ID   ARFA_HAEIN              Reviewed;          69 AA.
AC   P44588;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Alternative ribosome-rescue factor A;
GN   Name=arfA; OrderedLocusNames=HI_0235;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: Rescues ribosomes stalled at the 3' end of non-stop mRNAs.
CC       Recruits release factor 2 (RF2) to the stalled ribosome, helping
CC       position it correctly in the ribosomal A site so its GGQ motif can
CC       hydrolyze the peptidyl-tRNA bond (By similarity).
CC       {ECO:0000250|UniProtKB:P36675}.
CC   -!- SUBUNIT: Interacts with the 70S ribosome and release factor 2.
CC       {ECO:0000250|UniProtKB:P36675}.
CC   -!- SIMILARITY: Belongs to the alternative ribosome-rescue factor A family.
CC       {ECO:0000305}.
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DR   EMBL; L42023; AAC21905.1; -; Genomic_DNA.
DR   PIR; I64145; I64145.
DR   RefSeq; NP_438406.1; NC_000907.1.
DR   RefSeq; WP_005648813.1; NC_000907.1.
DR   AlphaFoldDB; P44588; -.
DR   SMR; P44588; -.
DR   STRING; 71421.HI_0235; -.
DR   DNASU; 951152; -.
DR   EnsemblBacteria; AAC21905; AAC21905; HI_0235.
DR   KEGG; hin:HI_0235; -.
DR   PATRIC; fig|71421.8.peg.250; -.
DR   eggNOG; COG3036; Bacteria.
DR   HOGENOM; CLU_170842_2_1_6; -.
DR   OMA; HAKGGNW; -.
DR   PhylomeDB; P44588; -.
DR   BioCyc; HINF71421:G1GJ1-251-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   GO; GO:0072344; P:rescue of stalled ribosome; IEA:InterPro.
DR   InterPro; IPR005589; ArfA.
DR   Pfam; PF03889; ArfA; 1.
PE   3: Inferred from homology;
KW   Reference proteome; RNA-binding; rRNA-binding; Translation regulation.
FT   CHAIN           1..69
FT                   /note="Alternative ribosome-rescue factor A"
FT                   /id="PRO_0000169495"
SQ   SEQUENCE   69 AA;  8154 MW;  786EC512B63540B1 CRC64;
     MRKKQKSAVE NETVYLHTRG VIKDNAVMAL LGDKLFRQRV EKKRKGKGSY QRKAKHPGKI
     FEKPDYKFF