ID   ARFA_MYCBP              Reviewed;         326 AA.
AC   A1KH31;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Peptidoglycan-binding protein ArfA;
DE   AltName: Full=Outer membrane protein A;
DE            Short=OmpATb;
DE   AltName: Full=Outer membrane protein ArfA;
GN   Name=arfA; Synonyms=ompA; OrderedLocusNames=BCG_0951;
OS   Mycobacterium bovis (strain BCG / Pasteur 1173P2).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=410289;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BCG / Pasteur 1173P2;
RX   PubMed=17372194; DOI=10.1073/pnas.0700869104;
RA   Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P.,
RA   Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K.,
RA   Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C.,
RA   Barrell B.G., Parkhill J., Cole S.T.;
RT   "Genome plasticity of BCG and impact on vaccine efficacy.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007).
RN   [2]
RP   INDUCTION, REQUIREMENT FOR ARFB AND ARFC, AND DISRUPTION PHENOTYPE.
RC   STRAIN=BCG / Pasteur 1173P2;
RX   PubMed=21802366; DOI=10.1016/j.tube.2011.06.008;
RA   Veyron-Churlet R., Brust B., Kremer L., Blanc-Potard A.B.;
RT   "Expression of OmpATb is dependent on small membrane proteins in
RT   Mycobacterium bovis BCG.";
RL   Tuberculosis 91:544-548(2011).
CC   -!- FUNCTION: May play a role in ammonia secretion that neutralizes the
CC       medium at pH 5.5, although it does not play a direct role in ammonia
CC       transport. Binds peptidoglycan (By similarity). Expression depends on
CC       arfB and arfC. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}. Note=Does not
CC       have a cleavable signal sequence. {ECO:0000250}.
CC   -!- INDUCTION: Expressed equally at pH 5.5 and 7.2 (at protein level). Part
CC       of the arfA-arfB-arfC operon. {ECO:0000269|PubMed:21802366}.
CC   -!- DISRUPTION PHENOTYPE: No effect of a single gene deletion on growth at
CC       pH 7.0 nor of whole-operon deletion on growth at pH 4.6, 5.5 or 7.2. No
CC       effect of whole-operon deletion on growth in macrophages.
CC       {ECO:0000269|PubMed:21802366}.
CC   -!- SIMILARITY: Belongs to the outer membrane OOP (TC 1.B.6) superfamily.
CC       ArfA family. {ECO:0000305}.
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DR   EMBL; AM408590; CAL70937.1; -; Genomic_DNA.
DR   RefSeq; WP_003404684.1; NC_008769.1.
DR   AlphaFoldDB; A1KH31; -.
DR   BMRB; A1KH31; -.
DR   SMR; A1KH31; -.
DR   KEGG; mbb:BCG_0951; -.
DR   HOGENOM; CLU_836308_0_0_11; -.
DR   OMA; MKGEVHN; -.
DR   Proteomes; UP000001472; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   CDD; cd07185; OmpA_C-like; 1.
DR   Gene3D; 3.30.1330.60; -; 1.
DR   InterPro; IPR007055; BON_dom.
DR   InterPro; IPR006664; OMP_bac.
DR   InterPro; IPR006665; OmpA-like.
DR   InterPro; IPR006690; OMPA-like_CS.
DR   InterPro; IPR036737; OmpA-like_sf.
DR   Pfam; PF04972; BON; 1.
DR   Pfam; PF00691; OmpA; 1.
DR   PRINTS; PR01021; OMPADOMAIN.
DR   SUPFAM; SSF103088; SSF103088; 1.
DR   PROSITE; PS01068; OMPA_1; 1.
DR   PROSITE; PS51123; OMPA_2; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Disulfide bond; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..326
FT                   /note="Peptidoglycan-binding protein ArfA"
FT                   /id="PRO_0000415885"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          127..196
FT                   /note="BON"
FT   DOMAIN          212..326
FT                   /note="OmpA-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00473"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..20
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        208..250
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   326 AA;  33574 MW;  9E0D46ABCC179F4A CRC64;
     MASKAGLGQT PATTDARRTQ KFYRGSPGRP WLIGAVVIPL LIAAIGYGAF ERPQSVTGPT
     GVLPTLTPTS TRGASALSLS LLSISRSGNT VTLIGDFPDE AAKAALMTAL NGLLAPGVNV
     IDQIHVDPVV RSLDFSSAEP VFTASVPIPD FGLKVERDTV TLTGTAPSSE HKDAVKRAAT
     STWPDMKIVN NIEVTGQAPP GPPASGPCAD LQSAINAVTG GPIAFGNDGA SLIPADYEIL
     NRVADKLKAC PDARVTINGY TDNTGSEGIN IPLSAQRAKI VADYLVARGV AGDHIATVGL
     GSVNPIASNA TPEGRAKNRR VEIVVN