ARFB_ARATH
ID ARFB_ARATH Reviewed; 859 AA.
AC Q94JM3; O23671; Q56YL7; Q56YP4; Q56YR7; Q9C5W5; Q9FIT6;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 25-MAY-2022, entry version 152.
DE RecName: Full=Auxin response factor 2;
DE AltName: Full=ARF1-binding protein;
DE Short=ARF1-BP;
DE AltName: Full=Protein MEGAINTEGUMENTA;
GN Name=ARF2; Synonyms=MNT; OrderedLocusNames=At5g62000; ORFNames=MTG10.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=15659631; DOI=10.1105/tpc.104.028316;
RA Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C.,
RA Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A.,
RA Yu G., Theologis A.;
RT "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family
RT members in Arabidopsis thaliana: unique and overlapping functions of ARF7
RT and ARF19.";
RL Plant Cell 17:444-463(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 394-859.
RC STRAIN=cv. Columbia;
RX PubMed=9188533; DOI=10.1126/science.276.5320.1865;
RA Ulmasov T., Hagen G., Guilfoyle T.J.;
RT "ARF1, a transcription factor that binds to auxin response elements.";
RL Science 276:1865-1868(1997).
RN [7]
RP NUCLEOTIDE SEQUENCE OF 406-859.
RC STRAIN=cv. Columbia;
RA Sessa G., Carabelli M., Ciarbelli A.R., Ruzza V., Steindler C., Ruberti I.;
RT "Nucleotide sequence of the Arabidopsis IAA30.";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP DIMERIZATION, AND TISSUE SPECIFICITY.
RX PubMed=10476078; DOI=10.1046/j.1365-313x.1999.00538.x;
RA Ulmasov T., Hagen G., Guilfoyle T.J.;
RT "Dimerization and DNA binding of auxin response factors.";
RL Plant J. 19:309-319(1999).
RN [9]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036261; DOI=10.1023/a:1015207114117;
RA Hagen G., Guilfoyle T.J.;
RT "Auxin-responsive gene expression: genes, promoters and regulatory
RT factors.";
RL Plant Mol. Biol. 49:373-385(2002).
RN [10]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16176952; DOI=10.1242/dev.02012;
RA Ellis C.M., Nagpal P., Young J.C., Hagen G., Guilfoyle T.J., Reed J.W.;
RT "AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and
RT floral organ abscission in Arabidopsis thaliana.";
RL Development 132:4563-4574(2005).
RN [11]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15960614; DOI=10.1111/j.1365-313x.2005.02426.x;
RA Okushima Y., Mitina I., Quach H.L., Theologis A.;
RT "AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator.";
RL Plant J. 43:29-46(2005).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16339187; DOI=10.1242/dev.02194;
RA Schruff M.C., Spielman M., Tiwari S., Adams S., Fenby N., Scott R.J.;
RT "The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling,
RT cell division, and the size of seeds and other organs.";
RL Development 133:251-261(2006).
RN [13]
RP GENE FAMILY.
RX PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA Swaminathan K., Peterson K., Jack T.;
RT "The plant B3 superfamily.";
RL Trends Plant Sci. 13:647-655(2008).
CC -!- FUNCTION: Auxin response factors (ARFs) are transcriptional factors
CC that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the
CC auxin-responsive promoter elements (AuxREs). Could act as
CC transcriptional activator or repressor. Formation of heterodimers with
CC Aux/IAA proteins may alter their ability to modulate early auxin
CC response genes expression. Promotes flowering, stamen development,
CC floral organ abscission and fruit dehiscence. Functions independently
CC of ethylene and cytokinin response pathways. May act as a repressor of
CC cell division and organ growth. {ECO:0000269|PubMed:12036261,
CC ECO:0000269|PubMed:15960614, ECO:0000269|PubMed:16176952,
CC ECO:0000269|PubMed:16339187}.
CC -!- SUBUNIT: Homodimers and heterodimers. Interacts with ARF1.
CC -!- INTERACTION:
CC Q94JM3; Q8L7G0: ARF1; NbExp=4; IntAct=EBI-1799262, EBI-2324259;
CC Q94JM3; Q39011: ASK7; NbExp=6; IntAct=EBI-1799262, EBI-1798250;
CC Q94JM3; Q38828: IAA10; NbExp=6; IntAct=EBI-1799262, EBI-3946434;
CC Q94JM3; P93830: IAA17; NbExp=3; IntAct=EBI-1799262, EBI-632243;
CC Q94JM3; O24408: IAA18; NbExp=3; IntAct=EBI-1799262, EBI-2295525;
CC Q94JM3; Q8LAL2: IAA26; NbExp=7; IntAct=EBI-1799262, EBI-3947418;
CC Q94JM3; Q93WC4: IAA29; NbExp=6; IntAct=EBI-1799262, EBI-3946697;
CC Q94JM3; Q8RYC6: IAA32; NbExp=6; IntAct=EBI-1799262, EBI-3946448;
CC Q94JM3; Q9FKM7: IAA33; NbExp=3; IntAct=EBI-1799262, EBI-3946739;
CC Q94JM3; Q9C5X0: IAA34; NbExp=6; IntAct=EBI-1799262, EBI-3946459;
CC Q94JM3; Q9LQF0: TCP23; NbExp=3; IntAct=EBI-1799262, EBI-15192297;
CC Q94JM3; Q8GWF1: WRKY38; NbExp=3; IntAct=EBI-1799262, EBI-1993263;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q94JM3-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:10476078, ECO:0000269|PubMed:15960614}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the sepals and carpels of young
CC flower buds. At stage 10 of flower development, expression in the
CC carpels becomes restricted to the style. Also expressed in anthers and
CC filaments. At stage 13, expressed in the region at the top of the
CC pedicel, including the abscission zone. Expressed in developing
CC siliques. {ECO:0000269|PubMed:15960614, ECO:0000269|PubMed:16176952}.
CC -!- DOMAIN: Interactions between auxin response factors (ARFs) and Aux/IAA
CC proteins occur through their C-terminal dimerization domains III and
CC IV.
CC -!- DISRUPTION PHENOTYPE: Large, dark green rosette leaves, delayed
CC flowering, thick and long inflorescence, abnormal flower morphology and
CC sterility in early formed flowers, but fertility in late-formed
CC flowers. Delayed senescence and abscission. Increased seed size and
CC weight, and extra cell division and expansion in many organs.
CC {ECO:0000269|PubMed:15960614, ECO:0000269|PubMed:16339187}.
CC -!- SIMILARITY: Belongs to the ARF family. {ECO:0000305}.
CC -!- CAUTION: Was originally (Ref.5) erroneously termed IAA26 and IAA30.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC49752.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAK55665.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAN46837.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAD29696.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAD30210.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY669787; AAT67071.1; -; mRNA.
DR EMBL; AB016880; BAB10162.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97548.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97549.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97550.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68885.1; -; Genomic_DNA.
DR EMBL; AF336918; AAG53999.1; -; mRNA.
DR EMBL; AF378862; AAK55665.1; ALT_FRAME; mRNA.
DR EMBL; BT000784; AAN31923.1; -; mRNA.
DR EMBL; BT001072; AAN46837.1; ALT_FRAME; mRNA.
DR EMBL; AK221252; BAD93891.1; -; mRNA.
DR EMBL; AK221254; BAD93897.1; -; mRNA.
DR EMBL; AK221274; BAD93959.1; -; mRNA.
DR EMBL; AK221277; BAD93968.1; -; mRNA.
DR EMBL; AK221282; BAD93985.1; -; mRNA.
DR EMBL; AK221305; BAD94058.1; -; mRNA.
DR EMBL; U89771; AAC49752.1; ALT_INIT; mRNA.
DR EMBL; AJ441313; CAD29696.1; ALT_INIT; mRNA.
DR EMBL; AJ458328; CAD30210.1; ALT_INIT; mRNA.
DR RefSeq; NP_001330603.1; NM_001345519.1. [Q94JM3-1]
DR RefSeq; NP_201006.2; NM_125593.5. [Q94JM3-1]
DR RefSeq; NP_851244.1; NM_180913.4. [Q94JM3-1]
DR RefSeq; NP_974980.1; NM_203251.4. [Q94JM3-1]
DR AlphaFoldDB; Q94JM3; -.
DR SMR; Q94JM3; -.
DR BioGRID; 21565; 30.
DR DIP; DIP-46014N; -.
DR IntAct; Q94JM3; 20.
DR STRING; 3702.AT5G62000.1; -.
DR iPTMnet; Q94JM3; -.
DR PaxDb; Q94JM3; -.
DR PRIDE; Q94JM3; -.
DR ProteomicsDB; 246955; -. [Q94JM3-1]
DR EnsemblPlants; AT5G62000.1; AT5G62000.1; AT5G62000. [Q94JM3-1]
DR EnsemblPlants; AT5G62000.2; AT5G62000.2; AT5G62000. [Q94JM3-1]
DR EnsemblPlants; AT5G62000.3; AT5G62000.3; AT5G62000. [Q94JM3-1]
DR EnsemblPlants; AT5G62000.5; AT5G62000.5; AT5G62000. [Q94JM3-1]
DR GeneID; 836321; -.
DR Gramene; AT5G62000.1; AT5G62000.1; AT5G62000. [Q94JM3-1]
DR Gramene; AT5G62000.2; AT5G62000.2; AT5G62000. [Q94JM3-1]
DR Gramene; AT5G62000.3; AT5G62000.3; AT5G62000. [Q94JM3-1]
DR Gramene; AT5G62000.5; AT5G62000.5; AT5G62000. [Q94JM3-1]
DR KEGG; ath:AT5G62000; -.
DR Araport; AT5G62000; -.
DR TAIR; locus:2174013; AT5G62000.
DR eggNOG; ENOG502QRXI; Eukaryota.
DR HOGENOM; CLU_002626_2_2_1; -.
DR InParanoid; Q94JM3; -.
DR OMA; ENWMPSG; -.
DR OrthoDB; 214403at2759; -.
DR PhylomeDB; Q94JM3; -.
DR PRO; PR:Q94JM3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q94JM3; baseline and differential.
DR Genevisible; Q94JM3; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010227; P:floral organ abscission; IMP:TAIR.
DR GO; GO:0010047; P:fruit dehiscence; IMP:TAIR.
DR GO; GO:0010150; P:leaf senescence; IMP:TAIR.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0048481; P:plant ovule development; IMP:TAIR.
DR GO; GO:0009911; P:positive regulation of flower development; IMP:TAIR.
DR GO; GO:1903288; P:positive regulation of potassium ion import across plasma membrane; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR CDD; cd10017; B3_DNA; 1.
DR Gene3D; 2.40.330.10; -; 1.
DR InterPro; IPR010525; ARF_dom.
DR InterPro; IPR044835; ARF_plant.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31384; PTHR31384; 1.
DR Pfam; PF02309; AUX_IAA; 1.
DR Pfam; PF06507; Auxin_resp; 1.
DR Pfam; PF02362; B3; 1.
DR SMART; SM01019; B3; 1.
DR SUPFAM; SSF101936; SSF101936; 1.
DR PROSITE; PS50863; B3; 1.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Auxin signaling pathway; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..859
FT /note="Auxin response factor 2"
FT /id="PRO_0000111506"
FT DOMAIN 733..817
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT DNA_BIND 164..266
FT /note="TF-B3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 396..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 687..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 829..859
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 396..414
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 416..431
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 711..736
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 845..859
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 491..492
FT /note="RR -> KK (in Ref. 6; AAC49752 and 7; CAD29696/
FT CAD30210)"
FT /evidence="ECO:0000305"
FT CONFLICT 520
FT /note="D -> E (in Ref. 6; AAC49752 and 7; CAD29696/
FT CAD30210)"
FT /evidence="ECO:0000305"
FT CONFLICT 596
FT /note="V -> A (in Ref. 5; BAD93968)"
FT /evidence="ECO:0000305"
FT CONFLICT 675
FT /note="R -> K (in Ref. 6; AAC49752 and 7; CAD29696/
FT CAD30210)"
FT /evidence="ECO:0000305"
FT CONFLICT 823
FT /note="P -> Q (in Ref. 6; AAC49752 and 7; CAD29696/
FT CAD30210)"
FT /evidence="ECO:0000305"
FT CONFLICT 826
FT /note="L -> V (in Ref. 5; BAD93959/BAD93897/BAD93891)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 859 AA; 95701 MW; 6B75171D7FF0E66B CRC64;
MASSEVSMKG NRGGDNFSSS GFSDPKETRN VSVAGEGQKS NSTRSAAAER ALDPEAALYR
ELWHACAGPL VTVPRQDDRV FYFPQGHIEQ VEASTNQAAE QQMPLYDLPS KLLCRVINVD
LKAEADTDEV YAQITLLPEA NQDENAIEKE APLPPPPRFQ VHSFCKTLTA SDTSTHGGFS
VLRRHADECL PPLDMSRQPP TQELVAKDLH ANEWRFRHIF RGQPRRHLLQ SGWSVFVSSK
RLVAGDAFIF LRGENGELRV GVRRAMRQQG NVPSSVISSH SMHLGVLATA WHAISTGTMF
TVYYKPRTSP SEFIVPFDQY MESVKNNYSI GMRFKMRFEG EEAPEQRFTG TIVGIEESDP
TRWPKSKWRS LKVRWDETSS IPRPDRVSPW KVEPALAPPA LSPVPMPRPK RPRSNIAPSS
PDSSMLTREG TTKANMDPLP ASGLSRVLQG QEYSTLRTKH TESVECDAPE NSVVWQSSAD
DDKVDVVSGS RRYGSENWMS SARHEPTYTD LLSGFGTNID PSHGQRIPFY DHSSSPSMPA
KRILSDSEGK FDYLANQWQM IHSGLSLKLH ESPKVPAATD ASLQGRCNVK YSEYPVLNGL
STENAGGNWP IRPRALNYYE EVVNAQAQAQ AREQVTKQPF TIQEETAKSR EGNCRLFGIP
LTNNMNGTDS TMSQRNNLND AAGLTQIASP KVQDLSDQSK GSKSTNDHRE QGRPFQTNNP
HPKDAQTKTN SSRSCTKVHK QGIALGRSVD LSKFQNYEEL VAELDRLFEF NGELMAPKKD
WLIVYTDEEN DMMLVGDDPW QEFCCMVRKI FIYTKEEVRK MNPGTLSCRS EEEAVVGEGS
DAKDAKSASN PSLSSAGNS
