ARFC_ARATH
ID ARFC_ARATH Reviewed; 608 AA.
AC O23661; O23670; O81087; Q33DU9;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Auxin response factor 3;
DE AltName: Full=Protein ETTIN;
GN Name=ARF3; Synonyms=ETT; OrderedLocusNames=At2g33860; ORFNames=T1B8.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=9188533; DOI=10.1126/science.276.5320.1865;
RA Ulmasov T., Hagen G., Guilfoyle T.J.;
RT "ARF1, a transcription factor that binds to auxin response elements.";
RL Science 276:1865-1868(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Wassilewskija;
RX PubMed=9409666; DOI=10.1242/dev.124.22.4481;
RA Sessions A., Nemhauser J.L., McCall A., Roe J.L., Feldmann K.A.,
RA Zambryski P.C.;
RT "ETTIN patterns the Arabidopsis floral meristem and reproductive organs.";
RL Development 124:4481-4491(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-109.
RC STRAIN=cv. Columbia;
RX PubMed=16227452; DOI=10.1105/tpc.105.036533;
RA Nishimura T., Wada T., Yamamoto K.T., Okada K.;
RT "The Arabidopsis STV1 protein, responsible for translation reinitiation, is
RT required for auxin-mediated gynoecium patterning.";
RL Plant Cell 17:2940-2953(2005).
RN [7]
RP DIMERIZATION, AND TISSUE SPECIFICITY.
RX PubMed=10476078; DOI=10.1046/j.1365-313x.1999.00538.x;
RA Ulmasov T., Hagen G., Guilfoyle T.J.;
RT "Dimerization and DNA binding of auxin response factors.";
RL Plant J. 19:309-319(1999).
RN [8]
RP FUNCTION.
RX PubMed=10952886; DOI=10.1242/dev.127.18.3877;
RA Nemhauser J.L., Feldman L.J., Zambryski P.C.;
RT "Auxin and ETTIN in Arabidopsis gynoecium morphogenesis.";
RL Development 127:3877-3888(2000).
RN [9]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036261; DOI=10.1023/a:1015207114117;
RA Hagen G., Guilfoyle T.J.;
RT "Auxin-responsive gene expression: genes, promoters and regulatory
RT factors.";
RL Plant Mol. Biol. 49:373-385(2002).
RN [10]
RP GENE FAMILY.
RX PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA Swaminathan K., Peterson K., Jack T.;
RT "The plant B3 superfamily.";
RL Trends Plant Sci. 13:647-655(2008).
RN [11]
RP INDUCTION.
RX PubMed=19956801; DOI=10.1371/journal.pbio.1000251;
RA Ng K.H., Yu H., Ito T.;
RT "AGAMOUS controls GIANT KILLER, a multifunctional chromatin modifier in
RT reproductive organ patterning and differentiation.";
RL PLoS Biol. 7:E1000251-E1000251(2009).
RN [12]
RP DISRUPTION PHENOTYPE.
RX PubMed=27334696; DOI=10.1242/bio.019109;
RA Matsumura Y., Ohbayashi I., Takahashi H., Kojima S., Ishibashi N., Keta S.,
RA Nakagawa A., Hayashi R., Saez-Vasquez J., Echeverria M., Sugiyama M.,
RA Nakamura K., Machida C., Machida Y.;
RT "A genetic link between epigenetic repressor AS1-AS2 and a putative small
RT subunit processome in leaf polarity establishment of Arabidopsis.";
RL Biol. Open 5:942-954(2016).
CC -!- FUNCTION: Auxin response factors (ARFs) are transcriptional factors
CC that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the
CC auxin-responsive promoter elements (AuxREs). Could act as
CC transcriptional activator or repressor. Formation of heterodimers with
CC Aux/IAA proteins may alter their ability to modulate early auxin
CC response genes expression. Involved in the establishment or elaboration
CC of tissue patterning during gynoecial development.
CC {ECO:0000269|PubMed:10952886, ECO:0000269|PubMed:12036261}.
CC -!- SUBUNIT: Homo and heterodimers. {ECO:0000250}.
CC -!- INTERACTION:
CC O23661; Q9SA94: At1g11810; NbExp=3; IntAct=EBI-3946474, EBI-4433406;
CC O23661; Q9FFR5: MBK23.15; NbExp=3; IntAct=EBI-3946474, EBI-25521402;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:10476078, ECO:0000269|PubMed:9409666}.
CC -!- DEVELOPMENTAL STAGE: Detected during gynoecium development.
CC -!- INDUCTION: Negatively regulated by AHL21/GIK.
CC {ECO:0000269|PubMed:19956801}.
CC -!- DISRUPTION PHENOTYPE: Double mutations in this protein and in ARF4
CC protein significantly suppresses the adaxial development defect of
CC leaves of the AS2 and RH10 proteins double mutant at high temperatures.
CC {ECO:0000269|PubMed:27334696}.
CC -!- SIMILARITY: Belongs to the ARF family. {ECO:0000305}.
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DR EMBL; U89296; AAB62404.1; -; mRNA.
DR EMBL; AF007788; AAC23589.1; -; Genomic_DNA.
DR EMBL; U78721; AAC69148.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08900.1; -; Genomic_DNA.
DR EMBL; AF336917; AAG53998.1; -; mRNA.
DR EMBL; AF360313; AAK26023.1; -; mRNA.
DR EMBL; AY056365; AAL07251.1; -; mRNA.
DR EMBL; AB199791; BAE48151.1; -; mRNA.
DR PIR; E84750; E84750.
DR PIR; T03278; T03278.
DR RefSeq; NP_180942.1; NM_128946.4.
DR AlphaFoldDB; O23661; -.
DR SMR; O23661; -.
DR BioGRID; 3303; 32.
DR IntAct; O23661; 21.
DR STRING; 3702.AT2G33860.1; -.
DR iPTMnet; O23661; -.
DR PaxDb; O23661; -.
DR PRIDE; O23661; -.
DR ProteomicsDB; 241022; -.
DR EnsemblPlants; AT2G33860.1; AT2G33860.1; AT2G33860.
DR GeneID; 817956; -.
DR Gramene; AT2G33860.1; AT2G33860.1; AT2G33860.
DR KEGG; ath:AT2G33860; -.
DR Araport; AT2G33860; -.
DR TAIR; locus:2057609; AT2G33860.
DR eggNOG; ENOG502QQSY; Eukaryota.
DR HOGENOM; CLU_002626_2_2_1; -.
DR InParanoid; O23661; -.
DR OMA; DTDPMWH; -.
DR OrthoDB; 266861at2759; -.
DR PhylomeDB; O23661; -.
DR PRO; PR:O23661; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O23661; baseline and differential.
DR Genevisible; O23661; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0031490; F:chromatin DNA binding; IPI:TAIR.
DR GO; GO:0003677; F:DNA binding; ISS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010158; P:abaxial cell fate specification; IGI:TAIR.
DR GO; GO:0009850; P:auxin metabolic process; TAS:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010582; P:floral meristem determinacy; IMP:TAIR.
DR GO; GO:1902584; P:positive regulation of response to water deprivation; IMP:TAIR.
DR GO; GO:0009733; P:response to auxin; ISS:TAIR.
DR GO; GO:0010050; P:vegetative phase change; IMP:TAIR.
DR CDD; cd10017; B3_DNA; 1.
DR DisProt; DP02380; -.
DR Gene3D; 2.40.330.10; -; 1.
DR InterPro; IPR010525; ARF_dom.
DR InterPro; IPR044835; ARF_plant.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR PANTHER; PTHR31384; PTHR31384; 1.
DR Pfam; PF06507; Auxin_resp; 1.
DR Pfam; PF02362; B3; 1.
DR SMART; SM01019; B3; 1.
DR SUPFAM; SSF101936; SSF101936; 1.
DR PROSITE; PS50863; B3; 1.
PE 1: Evidence at protein level;
KW Auxin signaling pathway; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..608
FT /note="Auxin response factor 3"
FT /id="PRO_0000111507"
FT DNA_BIND 159..261
FT /note="TF-B3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 411
FT /note="F -> I (in Ref. 2; AAC23589)"
FT /evidence="ECO:0000305"
FT CONFLICT 530
FT /note="L -> F (in Ref. 1; AAB62404)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 608 AA; 66606 MW; 4BE93292324D354B CRC64;
MGGLIDLNVM ETEEDETQTQ TPSSASGSVS PTSSSSASVS VVSSNSAGGG VCLELWHACA
GPLISLPKRG SLVLYFPQGH LEQAPDFSAA IYGLPPHVFC RILDVKLHAE TTTDEVYAQV
SLLPESEDIE RKVREGIIDV DGGEEDYEVL KRSNTPHMFC KTLTASDTST HGGFSVPRRA
AEDCFPPLDY SQPRPSQELL ARDLHGLEWR FRHIYRGQPR RHLLTTGWSA FVNKKKLVSG
DAVLFLRGDD GKLRLGVRRA SQIEGTAALS AQYNQNMNHN NFSEVAHAIS THSVFSISYN
PKASWSNFII PAPKFLKVVD YPFCIGMRFK ARVESEDASE RRSPGIISGI SDLDPIRWPG
SKWRCLLVRW DDIVANGHQQ RVSPWEIEPS GSISNSGSFV TTGPKRSRIG FSSGKPDIPV
SEGIRATDFE ESLRFQRVLQ GQEIFPGFIN TCSDGGAGAR RGRFKGTEFG DSYGFHKVLQ
GQETVPAYSI TDHRQQHGLS QRNIWCGPFQ NFSTRILPPS VSSSPSSVLL TNSNSPNGRL
EDHHGGSGRC RLFGFPLTDE TTAVASATAV PCVEGNSMKG ASAVQSNHHH SQGRDIYAMR
DMLLDIAL