ID   LEPC_ASPFN              Reviewed;         475 AA.
AC   B8NJG7;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Leporins efflux protein lepC {ECO:0000303|PubMed:26051490};
DE   AltName: Full=Leporins biosynthesis protein C {ECO:0000303|PubMed:26051490};
GN   Name=lepC {ECO:0000303|PubMed:26051490}; ORFNames=AFLA_066880;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
RN   [2]
RP   IDENTIFICATION OF THE GENE CLUSTER 23, AND FUNCTION.
RX   PubMed=20447271; DOI=10.1111/j.1364-3703.2009.00594.x;
RA   Georgianna D.R., Fedorova N.D., Burroughs J.L., Dolezal A.L., Bok J.W.,
RA   Horowitz-Brown S., Woloshuk C.P., Yu J., Keller N.P., Payne G.A.;
RT   "Beyond aflatoxin: four distinct expression patterns and functional roles
RT   associated with Aspergillus flavus secondary metabolism gene clusters.";
RL   Mol. Plant Pathol. 11:213-226(2010).
RN   [3]
RP   FUNCTION.
RX   PubMed=26051490; DOI=10.1016/j.fgb.2015.05.010;
RA   Cary J.W., Uka V., Han Z., Buyst D., Harris-Coward P.Y., Ehrlich K.C.,
RA   Wei Q., Bhatnagar D., Dowd P.F., Martens S.L., Calvo A.M., Martins J.C.,
RA   Vanhaecke L., Coenye T., De Saeger S., Di Mavungu J.D.;
RT   "An Aspergillus flavus secondary metabolic gene cluster containing a hybrid
RT   PKS-NRPS is necessary for synthesis of the 2-pyridones, leporins.";
RL   Fungal Genet. Biol. 81:88-97(2015).
CC   -!- FUNCTION: Efflux pump that may be involved in the secretion of leporins
CC       (PubMed:20447271, PubMed:26051490). {ECO:0000269|PubMed:26051490,
CC       ECO:0000305|PubMed:20447271}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:26051490};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Monocarboxylate porter (TC 2.A.1.13) family. {ECO:0000305}.
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DR   EMBL; EQ963479; EED49866.1; -; Genomic_DNA.
DR   RefSeq; XP_002380247.1; XM_002380206.1.
DR   AlphaFoldDB; B8NJG7; -.
DR   SMR; B8NJG7; -.
DR   EnsemblFungi; EED49866; EED49866; AFLA_066880.
DR   VEuPathDB; FungiDB:AFLA_066880; -.
DR   eggNOG; KOG2504; Eukaryota.
DR   HOGENOM; CLU_001265_1_1_1; -.
DR   OMA; FYHATIL; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..475
FT                   /note="Leporins efflux protein lepC"
FT                   /id="PRO_0000438461"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        255..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        283..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        329..349
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        443..463
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          216..242
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        216..239
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   475 AA;  50776 MW;  E7DD36F1976B4A30 CRC64;
     MSATVDNTEA NASPPKEFTL RSTIALIGAF MALFCTLGFQ NAFGVFQAFY HATILRDHSE
     FDIAWIGSLL TFMIFFFAAP AGVLVDRVGP THSLTRHLQP LLTFGAIATI LATFMISLCK
     ELYQFLLAQG ILLGIGNAFL LCPAMATVTR LFDHHRGAAN GIMIAGSSIG GIIWPIMLDQ
     LLNKDGVSFG WTFRIVGFVV LPLCLFMVAT IRPAPKTPHD SDREGIELSH GESESDHKAQ
     GAEGPAAIIK NPTFLILCAG LSVATFGLFS PLFFISTYAT DQGLSVSLAF YLVSMLNGAS
     MVGRVSTGFL ADRYGNFNLC FLTIASSGLI AMCWTKATNT VGIIFFALAY GYTSGVSPSD
     LKKNPPGPNT AVAGHVQSPN PVCRPALDAR VEGGCRRHSL CRASNSVSIV LFSRIARSCR
     LTHSYSGLVG TPISGRLLKH GYLALSMYSG AALLAGAGLV LIARLRQNTK LMAKV