ID   LEPE_ASPFN              Reviewed;         828 AA.
AC   B8NJG9;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Transcription factor lepE {ECO:0000303|PubMed:26051490};
DE   AltName: Full=Leporins biosynthesis protein E {ECO:0000303|PubMed:26051490};
GN   Name=lepB {ECO:0000303|PubMed:26051490}; ORFNames=AFLA_066900;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
RN   [2]
RP   IDENTIFICATION OF THE GENE CLUSTER 23, AND FUNCTION.
RX   PubMed=20447271; DOI=10.1111/j.1364-3703.2009.00594.x;
RA   Georgianna D.R., Fedorova N.D., Burroughs J.L., Dolezal A.L., Bok J.W.,
RA   Horowitz-Brown S., Woloshuk C.P., Yu J., Keller N.P., Payne G.A.;
RT   "Beyond aflatoxin: four distinct expression patterns and functional roles
RT   associated with Aspergillus flavus secondary metabolism gene clusters.";
RL   Mol. Plant Pathol. 11:213-226(2010).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26051490; DOI=10.1016/j.fgb.2015.05.010;
RA   Cary J.W., Uka V., Han Z., Buyst D., Harris-Coward P.Y., Ehrlich K.C.,
RA   Wei Q., Bhatnagar D., Dowd P.F., Martens S.L., Calvo A.M., Martins J.C.,
RA   Vanhaecke L., Coenye T., De Saeger S., Di Mavungu J.D.;
RT   "An Aspergillus flavus secondary metabolic gene cluster containing a hybrid
RT   PKS-NRPS is necessary for synthesis of the 2-pyridones, leporins.";
RL   Fungal Genet. Biol. 81:88-97(2015).
CC   -!- FUNCTION: Transcription factor involved in regulation of gene cluster
CC       23 that mediates the biosynthesis of leporins (PubMed:20447271,
CC       PubMed:26051490). {ECO:0000269|PubMed:26051490,
CC       ECO:0000305|PubMed:20447271}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Results in significantly reduced expression of
CC       the leporins biosynthesis gene cluster (PubMed:26051490).
CC       {ECO:0000269|PubMed:26051490}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EQ963479; EED49868.1; -; Genomic_DNA.
DR   RefSeq; XP_002380249.1; XM_002380208.1.
DR   AlphaFoldDB; B8NJG9; -.
DR   SMR; B8NJG9; -.
DR   EnsemblFungi; EED49868; EED49868; AFLA_066900.
DR   VEuPathDB; FungiDB:AFLA_066900; -.
DR   eggNOG; ENOG502SMMJ; Eukaryota.
DR   HOGENOM; CLU_007091_2_0_1; -.
DR   OMA; HQPMVAR; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..828
FT                   /note="Transcription factor lepE"
FT                   /id="PRO_0000438458"
FT   DNA_BIND        23..51
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   828 AA;  92231 MW;  0AC601B5DA51BB6B CRC64;
     MDGSSRPLPQ TAQRKRRRPV LACDRCRRRK IRCDRKVPCS HCLRTGYAST CTYLANSNSS
     FPEEDLPGLD TTDPAMDMAT SHPNHGHDRV PTDSGIGHSP PAQVEPIPSE GEMYGDFWDP
     LFVIHNTIST RKHRDPASTP ASLGHVPRHT PAQAPTEVQV LIQRVRQLEQ KVFSQQEPES
     SLAAPHIQQR HPQARRTVCQ ANLLGKSHWL VGFAQFDTIV HTLNNRLQDN DVEFRALFVK
     CQRLSRAIQQ QYSLRASSLQ ALDGSLPSQE ACDVCVNAYC RTFESVLRIL HVPTFRQVYN
     EMWTGSQPTS RLFLLKLQLV IAIGARVVNQ SDMLGTPDMA SRCVAWIQEA QQWLHAPGES
     LQASVDGIQL YCLVLLTRIV CAVDASLVYV STGALLQKAQ QLGLHRDPSH FPAMPVFQAE
     IRRRLWCTVC ELVLQSSTDS GTPPLISCDE FDCRPPANLN DTNLGTTSEP EPSNILTQTS
     FSILLMRCLP VRVQVAKVLN SVQLDPSYSE VLRLGEELTA ACHYNTQMIK SMSPDHEQPT
     LFQLELLNLL TYRFLLCLHQ PFAMKARKNL AYYFSRRVCL ETAIQILAGH PTTPSGQNVN
     DYLNVKRYSS GLFRDPYLLA SVTVGHEILC VTEGDWFSCQ PVSPLTNNLS STPNKSQQVP
     RTVLEEYVGL SECRLKDGAQ IDIKTYVLLS CIIAQIKAAE TGSPQDDAVL SAAVDSLEKC
     HIVLVDRYKS LAQSRESPQP LQVYGGSNVP CRLGKGSNDI LDINETGEGE AWSDFDVSQV
     CVMCHSRYDL TSDCIQAEWS LQVEYPDLDW NLLNTWSACV PGSDSGAS