ID   ARFC_MYCBP              Reviewed;         175 AA.
AC   A1KH33;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Uncharacterized membrane protein ArfC;
GN   Name=arfC; OrderedLocusNames=BCG_0953;
OS   Mycobacterium bovis (strain BCG / Pasteur 1173P2).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=410289;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BCG / Pasteur 1173P2;
RX   PubMed=17372194; DOI=10.1073/pnas.0700869104;
RA   Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P.,
RA   Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K.,
RA   Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C.,
RA   Barrell B.G., Parkhill J., Cole S.T.;
RT   "Genome plasticity of BCG and impact on vaccine efficacy.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=BCG / Pasteur 1173P2;
RX   PubMed=21802366; DOI=10.1016/j.tube.2011.06.008;
RA   Veyron-Churlet R., Brust B., Kremer L., Blanc-Potard A.B.;
RT   "Expression of OmpATb is dependent on small membrane proteins in
RT   Mycobacterium bovis BCG.";
RL   Tuberculosis 91:544-548(2011).
CC   -!- FUNCTION: Required for ammonia secretion (By similarity). Also required
CC       for wild-type expression of ArfA. {ECO:0000250,
CC       ECO:0000269|PubMed:21802366}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:21802366};
CC       Single-pass membrane protein {ECO:0000305|PubMed:21802366}.
CC   -!- INDUCTION: Expressed equally at pH 5.5 and 7.2. Part of the arfA-arfB-
CC       arfC operon. {ECO:0000269|PubMed:21802366}.
CC   -!- DISRUPTION PHENOTYPE: No effect of a single gene deletion on growth at
CC       pH 7.0 nor of whole-operon deletion on growth at pH 4.6, 5.5 or 7.2. No
CC       effect of whole-operon deletion on growth in macrophages. Loss of
CC       expression of ArfA. {ECO:0000269|PubMed:21802366}.
CC   -!- SIMILARITY: Belongs to the ArfC membrane protein family. {ECO:0000305}.
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DR   EMBL; AM408590; CAL70939.1; -; Genomic_DNA.
DR   RefSeq; WP_003404688.1; NC_008769.1.
DR   AlphaFoldDB; A1KH33; -.
DR   SMR; A1KH33; -.
DR   KEGG; mbb:BCG_0953; -.
DR   HOGENOM; CLU_1523548_0_0_11; -.
DR   OMA; PEDECYE; -.
DR   Proteomes; UP000001472; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..175
FT                   /note="Uncharacterized membrane protein ArfC"
FT                   /id="PRO_0000415887"
FT   TRANSMEM        4..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          36..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   175 AA;  18909 MW;  AD7024B10E5E1704 CRC64;
     MEHVHWWLAG LAFTLGMVLT STLMVRPVEH QVLVKKSVRG SSAKSKPPTA RKPAVKSGTK
     REESPTAKTK VATESAAEQI PVAGEPAAEP IPVAGEPAAR IPVVPYAPYG PGSARAGADG
     SGPQGWLVKG RSDTRLYYTP EDPTYDPTVA QVWFQDEESA ARAFFTPWRK STRRT