ID   ARFC_MYCTU              Reviewed;         175 AA.
AC   P9WJG5; L0T6R9; P64757; Q10559;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Uncharacterized membrane protein ArfC;
GN   Name=arfC; OrderedLocusNames=Rv0901; ORFNames=MTCY31.29;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21410778; DOI=10.1111/j.1365-2958.2011.07619.x;
RA   Song H., Huff J., Janik K., Walter K., Keller C., Ehlers S., Bossmann S.H.,
RA   Niederweis M.;
RT   "Expression of the ompATb operon accelerates ammonia secretion and
RT   adaptation of Mycobacterium tuberculosis to acidic environments.";
RL   Mol. Microbiol. 80:900-918(2011).
CC   -!- FUNCTION: Required for wild-type expression of ArfA and ammonia
CC       secretion, not however part of an ammonia transporter.
CC       {ECO:0000269|PubMed:21410778}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass membrane
CC       protein {ECO:0000250}.
CC   -!- INDUCTION: Part of the arfA-arfB-arfC operon. Maximal expression of
CC       ArfA requires the full operon. {ECO:0000269|PubMed:21410778}.
CC   -!- DISRUPTION PHENOTYPE: Upon operon disruption no reduction of serine
CC       uptake at pH 6.9, no visible effect on outer membrane permeability,
CC       however severe delays in ammonia secretion, medium pH neutralization
CC       and growth also occur at pH 5.5. {ECO:0000269|PubMed:21410778}.
CC   -!- SIMILARITY: Belongs to the ArfC membrane protein family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43649.1; -; Genomic_DNA.
DR   PIR; B70783; B70783.
DR   RefSeq; NP_215416.1; NC_000962.3.
DR   RefSeq; WP_003404688.1; NZ_NVQJ01000001.1.
DR   AlphaFoldDB; P9WJG5; -.
DR   SMR; P9WJG5; -.
DR   STRING; 83332.Rv0901; -.
DR   PaxDb; P9WJG5; -.
DR   DNASU; 885203; -.
DR   GeneID; 885203; -.
DR   KEGG; mtu:Rv0901; -.
DR   TubercuList; Rv0901; -.
DR   eggNOG; COG0088; Bacteria.
DR   OMA; PEDECYE; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..175
FT                   /note="Uncharacterized membrane protein ArfC"
FT                   /id="PRO_0000103741"
FT   TRANSMEM        4..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          36..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   175 AA;  18909 MW;  AD7024B10E5E1704 CRC64;
     MEHVHWWLAG LAFTLGMVLT STLMVRPVEH QVLVKKSVRG SSAKSKPPTA RKPAVKSGTK
     REESPTAKTK VATESAAEQI PVAGEPAAEP IPVAGEPAAR IPVVPYAPYG PGSARAGADG
     SGPQGWLVKG RSDTRLYYTP EDPTYDPTVA QVWFQDEESA ARAFFTPWRK STRRT