LEP_ARATH
ID LEP_ARATH Reviewed; 211 AA.
AC Q9M644;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Ethylene-responsive transcription factor LEP;
DE AltName: Full=Protein LEAFY PETIOLE;
GN Name=LEP; Synonyms=ERF085; OrderedLocusNames=At5g13910; ORFNames=MAC12.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. C24;
RX PubMed=11044410; DOI=10.1242/dev.127.22.4971;
RA van der Graaff E., Dulk-Ras A.D., Hooykaas P.J.J., Keller B.;
RT "Activation tagging of the LEAFY PETIOLE gene affects leaf petiole
RT development in Arabidopsis thaliana.";
RL Development 127:4971-4980(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Pan Y., Gong W., Liu D., Fu Q., Mei W.-Q., Song W.-Q., Ma L.-G., Luo J.-C.,
RA Deng X.-W., Zhu Y.-X.;
RT "Molecular cloning, expression, phylogenetic and functional
RT characterization of the Arabidopsis AP2/EREBP transcription factor
RT family.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12472696; DOI=10.1046/j.1365-313x.2002.01470.x;
RA van der Graaff E., Hooykaas P.J.J., Keller B.;
RT "Activation tagging of the two closely linked genes LEP and VAS
RT independently affects vascular cell number.";
RL Plant J. 32:819-830(2002).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION BY IMBIBITION.
RX PubMed=16339853; DOI=10.1105/tpc.105.036707;
RA Ward J.M., Smith A.M., Shah P.K., Galanti S.E., Yi H., Demianski A.J.,
RA van der Graaff E., Keller B., Neff M.M.;
RT "A new role for the Arabidopsis AP2 transcription factor, LEAFY PETIOLE, in
RT gibberellin-induced germination is revealed by the misexpression of a
RT homologous gene, SOB2/DRN-LIKE.";
RL Plant Cell 18:29-39(2006).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Cell division-promoting factor involved in leaf blade
CC differentiation, inflorescence branching, as well as in carpel and
CC silique shape. Promotes the number of xylem cells. Regulates positively
CC the gibberellin signaling pathway leading to germination, hypocotyl
CC elongation, and leaf expansion. Probably acts as a transcriptional
CC activator. Binds to the GCC-box pathogenesis-related promoter element.
CC May be involved in the regulation of gene expression by stress factors
CC and by components of stress signal transduction pathways (By
CC similarity). {ECO:0000250, ECO:0000269|PubMed:11044410,
CC ECO:0000269|PubMed:12472696, ECO:0000269|PubMed:16339853}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in germinating seeds. Present in young
CC shoots, at low levels, especially in leaf primordia and developing leaf
CC blades. Also detected in vascular tissue, mostly in xylem, of young
CC leaves, petioles and hypocotyls. {ECO:0000269|PubMed:11044410,
CC ECO:0000269|PubMed:12472696, ECO:0000269|PubMed:16339853}.
CC -!- INDUCTION: By imbibition. {ECO:0000269|PubMed:16339853}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AF216581; AAF32292.1; -; Genomic_DNA.
DR EMBL; AY560852; AAT44919.1; -; mRNA.
DR EMBL; AB005230; BAB11119.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91958.1; -; Genomic_DNA.
DR EMBL; BT024919; ABD94075.1; -; mRNA.
DR RefSeq; NP_196895.1; NM_121394.2.
DR AlphaFoldDB; Q9M644; -.
DR SMR; Q9M644; -.
DR BioGRID; 16516; 10.
DR IntAct; Q9M644; 10.
DR STRING; 3702.AT5G13910.1; -.
DR iPTMnet; Q9M644; -.
DR PaxDb; Q9M644; -.
DR PRIDE; Q9M644; -.
DR ProteomicsDB; 250754; -.
DR EnsemblPlants; AT5G13910.1; AT5G13910.1; AT5G13910.
DR GeneID; 831238; -.
DR Gramene; AT5G13910.1; AT5G13910.1; AT5G13910.
DR KEGG; ath:AT5G13910; -.
DR Araport; AT5G13910; -.
DR TAIR; locus:2159068; AT5G13910.
DR eggNOG; ENOG502RYDI; Eukaryota.
DR HOGENOM; CLU_073466_1_0_1; -.
DR InParanoid; Q9M644; -.
DR OMA; HGFNHFG; -.
DR OrthoDB; 1047083at2759; -.
DR PhylomeDB; Q9M644; -.
DR PRO; PR:Q9M644; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9M644; baseline and differential.
DR Genevisible; Q9M644; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010030; P:positive regulation of seed germination; IMP:TAIR.
DR GO; GO:0009739; P:response to gibberellin; IMP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Ethylene signaling pathway;
KW Gibberellin signaling pathway; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..211
FT /note="Ethylene-responsive transcription factor LEP"
FT /id="PRO_0000297929"
FT DNA_BIND 19..76
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 74..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..108
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 211 AA; 23681 MW; 97CBA836C91B0E9B CRC64;
MNTTSSKSKK KQDDQVGTRF LGVRRRPWGR YAAEIRDPTT KERHWLGTFD TAEEAALAYD
RAARSMRGTR ARTNFVYSDM PPSSSVTSIV SPDDPPPPPP PPAPPSNDPV DYMMMFNQYS
STDSPMLQPH CDQVDSYMFG GSQSSNSYCY SNDSSNELPP LPSDLSNSCY SQPQWTWTGD
DYSSEYVHSP MFSRMPPVSD SFPQGFNYFG S
