ID   LEP_HELPY               Reviewed;         290 AA.
AC   O25300;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 128.
DE   RecName: Full=Signal peptidase I;
DE            Short=SPase I;
DE            EC=3.4.21.89;
DE   AltName: Full=Leader peptidase I;
GN   Name=lepB; OrderedLocusNames=HP_0576;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences
CC         from secreted and periplasmic proteins.; EC=3.4.21.89;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S26 family. {ECO:0000305}.
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DR   EMBL; AE000511; AAD07643.1; -; Genomic_DNA.
DR   PIR; H64591; H64591.
DR   RefSeq; NP_207371.1; NC_000915.1.
DR   RefSeq; WP_000670710.1; NC_018939.1.
DR   AlphaFoldDB; O25300; -.
DR   SMR; O25300; -.
DR   IntAct; O25300; 2.
DR   STRING; 85962.C694_02970; -.
DR   PaxDb; O25300; -.
DR   EnsemblBacteria; AAD07643; AAD07643; HP_0576.
DR   KEGG; hpy:HP_0576; -.
DR   PATRIC; fig|85962.47.peg.621; -.
DR   eggNOG; COG0681; Bacteria.
DR   OMA; SDSRFWG; -.
DR   PhylomeDB; O25300; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006465; P:signal peptide processing; IBA:GO_Central.
DR   CDD; cd06530; S26_SPase_I; 1.
DR   InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR   InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR   InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR   InterPro; IPR019533; Peptidase_S26.
DR   PANTHER; PTHR43390; PTHR43390; 1.
DR   Pfam; PF10502; Peptidase_S26; 1.
DR   PRINTS; PR00727; LEADERPTASE.
DR   SUPFAM; SSF51306; SSF51306; 1.
DR   TIGRFAMs; TIGR02227; sigpep_I_bact; 1.
DR   PROSITE; PS00761; SPASE_I_3; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Protease; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..290
FT                   /note="Signal peptidase I"
FT                   /id="PRO_0000109508"
FT   TOPO_DOM        1..13
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        35..290
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        38
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        106
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   290 AA;  33710 MW;  F3DF2DFCE8465310 CRC64;
     MKFLRSVYAF CSSWVGTIVI VLLVIFFIAQ AFIIPSRSMV GTLYEGDMLF VKKFSYGIPI
     PKIPWIELPV MPDFKNNGHL IEGDRPKRGE VVVFIPPHEK KSYYVKRNFA IGGDEVLFTN
     EGFYLHPFES DTDKNYIAKH YPNAMTKEFM GKIFVLNPYK NEHPGIHYQK DNETFHLMEQ
     LATQGAEANI SMQLIQMEGE KVFYKKINDD EFFMIGDNRD NSSDSRFWGS VAYKNIVGSP
     WFVYFSLSLK NSLEMDAENN PKKRYLVRWE RMFKSVGGLE KIIKKENATH