ID   LEP_MYCLE               Reviewed;         289 AA.
AC   O33021;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 133.
DE   RecName: Full=Probable signal peptidase I;
DE            Short=SPase I;
DE            EC=3.4.21.89;
DE   AltName: Full=Leader peptidase I;
GN   Name=lepB; OrderedLocusNames=ML1612; ORFNames=MLCB250.39;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences
CC         from secreted and periplasmic proteins.; EC=3.4.21.89;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type II
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S26 family. {ECO:0000305}.
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DR   EMBL; Z97369; CAB10633.1; -; Genomic_DNA.
DR   EMBL; AL583922; CAC30563.1; -; Genomic_DNA.
DR   PIR; F87110; F87110.
DR   RefSeq; NP_302111.1; NC_002677.1.
DR   RefSeq; WP_010908432.1; NC_002677.1.
DR   AlphaFoldDB; O33021; -.
DR   SMR; O33021; -.
DR   STRING; 272631.ML1612; -.
DR   MEROPS; S26.024; -.
DR   EnsemblBacteria; CAC30563; CAC30563; CAC30563.
DR   KEGG; mle:ML1612; -.
DR   PATRIC; fig|272631.5.peg.3038; -.
DR   Leproma; ML1612; -.
DR   eggNOG; COG0681; Bacteria.
DR   HOGENOM; CLU_028723_0_0_11; -.
DR   OMA; HGCNGCV; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR   CDD; cd06530; S26_SPase_I; 1.
DR   InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR   InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR   InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR   InterPro; IPR019756; Pept_S26A_signal_pept_1_Ser-AS.
DR   InterPro; IPR019533; Peptidase_S26.
DR   PANTHER; PTHR43390; PTHR43390; 1.
DR   Pfam; PF10502; Peptidase_S26; 1.
DR   PRINTS; PR00727; LEADERPTASE.
DR   SUPFAM; SSF51306; SSF51306; 1.
DR   TIGRFAMs; TIGR02227; sigpep_I_bact; 1.
DR   PROSITE; PS00501; SPASE_I_1; 1.
DR   PROSITE; PS00761; SPASE_I_3; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Protease; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..289
FT                   /note="Probable signal peptidase I"
FT                   /id="PRO_0000109510"
FT   TOPO_DOM        1..53
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        75..289
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        84
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        162
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   289 AA;  31086 MW;  69F59B03944768DD CRC64;
     MTETTDSVPE PPSDADQLQP KVSICGLDMP AEVSETAAEA AIGVSEPKKR SALWEFAILA
     VIAIGLYYVM LTFVARPYLI PSESMEPTLH GCSGCVGDRI MVDKITYRFS SPQPGDVIVF
     KGPPSWNTMY KSIRSNNIVL RSVQNALSFV GFVPPDENDL VKRVIAVGGQ TVQCRSDTGL
     TVNGKPLKEP YLRPVTMNAD LSFSPCLGSE FGPVTVPQGR LWVMGDNRIH SADSRYHCNS
     TDVVNGLSCT GDPNSGTVPV SNVIGKARVV VWPPSRWGGV GSVNSQQGQ