ID   LEP_PSEAE               Reviewed;         284 AA.
AC   Q9I5G7;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Signal peptidase I;
DE            Short=SPase I;
DE            EC=3.4.21.89;
DE   AltName: Full=Leader peptidase I;
GN   Name=lepB; OrderedLocusNames=PA0768;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences
CC         from secreted and periplasmic proteins.; EC=3.4.21.89;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the peptidase S26 family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG04157.1; -; Genomic_DNA.
DR   PIR; H83550; H83550.
DR   RefSeq; NP_249459.1; NC_002516.2.
DR   RefSeq; WP_003101969.1; NZ_QZGE01000007.1.
DR   AlphaFoldDB; Q9I5G7; -.
DR   SMR; Q9I5G7; -.
DR   STRING; 287.DR97_1215; -.
DR   MEROPS; S26.001; -.
DR   PaxDb; Q9I5G7; -.
DR   PRIDE; Q9I5G7; -.
DR   DNASU; 882104; -.
DR   EnsemblBacteria; AAG04157; AAG04157; PA0768.
DR   GeneID; 882104; -.
DR   KEGG; pae:PA0768; -.
DR   PATRIC; fig|208964.12.peg.798; -.
DR   PseudoCAP; PA0768; -.
DR   HOGENOM; CLU_028723_1_1_6; -.
DR   InParanoid; Q9I5G7; -.
DR   OMA; SDSRFWG; -.
DR   PhylomeDB; Q9I5G7; -.
DR   BioCyc; PAER208964:G1FZ6-781-MON; -.
DR   BRENDA; 3.4.21.89; 5087.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0008233; F:peptidase activity; IDA:PseudoCAP.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006465; P:signal peptide processing; IBA:GO_Central.
DR   CDD; cd06530; S26_SPase_I; 1.
DR   InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR   InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR   InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR   InterPro; IPR019757; Pept_S26A_signal_pept_1_Lys-AS.
DR   InterPro; IPR019756; Pept_S26A_signal_pept_1_Ser-AS.
DR   InterPro; IPR019533; Peptidase_S26.
DR   PANTHER; PTHR43390; PTHR43390; 1.
DR   Pfam; PF10502; Peptidase_S26; 1.
DR   PRINTS; PR00727; LEADERPTASE.
DR   SUPFAM; SSF51306; SSF51306; 1.
DR   TIGRFAMs; TIGR02227; sigpep_I_bact; 1.
DR   PROSITE; PS00501; SPASE_I_1; 1.
DR   PROSITE; PS00760; SPASE_I_2; 1.
DR   PROSITE; PS00761; SPASE_I_3; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Protease;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..284
FT                   /note="Signal peptidase I"
FT                   /id="PRO_0000109513"
FT   TRANSMEM        4..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..58
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        78..284
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        90
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        145
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   284 AA;  32103 MW;  ACA143F48F90ED2C CRC64;
     MTLNFPLLLV IAVAVCGALA LVDLVLFAPR RRAAISSYEG QVNEPDPAVL EKLNKEPLLV
     EYGKSFFPVL FIVLVLRSFL VEPFQIPSGS MKPTLEVGDF ILVNKFAYGI RLPVLDTKVI
     PIGDPQRGDV MVFRYPSEPN INYIKRVVGL PGDTVRYTKE KRLYVNGELV AEKLVGEEPG
     TLGSVTLYQE KLGQAEHLIR KEMSRYRIEP DRQWTIPAGH YFMMGDNRDN SNDSRYWNDP
     KIPKDLLGMV PDRNIVGKAF AVWMSWPDPK MSNLPNFSRV GVIH