LEP_SALTY
ID LEP_SALTY Reviewed; 324 AA.
AC P0A1W2; P23697;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Signal peptidase I;
DE Short=SPase I;
DE EC=3.4.21.89;
DE AltName: Full=Leader peptidase I;
GN Name=lepB; OrderedLocusNames=STM2582;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PP1139;
RX PubMed=2250650; DOI=10.1007/bf00265059;
RA van Dijl J.M., van den Bergh R., Reversma T., Smith H., Bron S., Venema G.;
RT "Molecular cloning of the Salmonella typhimurium lep gene in Escherichia
RT coli.";
RL Mol. Gen. Genet. 223:233-240(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences
CC from secreted and periplasmic proteins.; EC=3.4.21.89;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase S26 family. {ECO:0000305}.
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DR EMBL; X54933; CAA38694.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21476.1; -; Genomic_DNA.
DR PIR; S12020; S12020.
DR RefSeq; NP_461517.1; NC_003197.2.
DR RefSeq; WP_000002559.1; NC_003197.2.
DR AlphaFoldDB; P0A1W2; -.
DR SMR; P0A1W2; -.
DR STRING; 99287.STM2582; -.
DR MEROPS; S26.001; -.
DR PaxDb; P0A1W2; -.
DR EnsemblBacteria; AAL21476; AAL21476; STM2582.
DR GeneID; 1254104; -.
DR KEGG; stm:STM2582; -.
DR PATRIC; fig|99287.12.peg.2723; -.
DR HOGENOM; CLU_028723_1_1_6; -.
DR OMA; SDSRFWG; -.
DR PhylomeDB; P0A1W2; -.
DR BioCyc; SENT99287:STM2582-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006465; P:signal peptide processing; IBA:GO_Central.
DR CDD; cd06530; S26_SPase_I; 1.
DR Gene3D; 2.170.230.10; -; 1.
DR InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR InterPro; IPR019757; Pept_S26A_signal_pept_1_Lys-AS.
DR InterPro; IPR019756; Pept_S26A_signal_pept_1_Ser-AS.
DR InterPro; IPR019533; Peptidase_S26.
DR InterPro; IPR019766; Sign_pep_all-beta_subdom.
DR PANTHER; PTHR43390; PTHR43390; 1.
DR Pfam; PF10502; Peptidase_S26; 1.
DR PRINTS; PR00727; LEADERPTASE.
DR SUPFAM; SSF51306; SSF51306; 1.
DR TIGRFAMs; TIGR02227; sigpep_I_bact; 2.
DR PROSITE; PS00501; SPASE_I_1; 1.
DR PROSITE; PS00760; SPASE_I_2; 1.
DR PROSITE; PS00761; SPASE_I_3; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Hydrolase; Membrane; Protease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..324
FT /note="Signal peptidase I"
FT /id="PRO_0000109518"
FT TOPO_DOM 1..3
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 4..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..324
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT ACT_SITE 91
FT /evidence="ECO:0000250"
FT ACT_SITE 146
FT /evidence="ECO:0000250"
SQ SEQUENCE 324 AA; 35778 MW; 40607CDA15779575 CRC64;
MANMFALILV IATLVTGILW CVDKFVFAPK RRARQAAAQT ASGDALDNAT LNKVAPKPGW
LETGASVFPV LAIVLIVRSF LYEPFQIPSG SMMPTLLIGD FILVEKFAYG IKDPIYQKTL
IETGHPKRGD IVVFKYPEDP KLDYIKRAVG LPGDKITYDP VAKEVTIQPG CSSGQACENA
LPVTYSNVEP SDFVQTFARR NGGEATSGFF EVPLNETKEN GIRLTERKET LGDVTHRILM
VPIAQDQLGM YYQQPGQPLA TWVVPPGQYF MMGDNRDNSA DSRYWGFVPE ANLVGKAVAI
WMSFDKQEGE WPTGVRLSRI GGIH
