ARFF_ARATH
ID ARFF_ARATH Reviewed; 935 AA.
AC Q9ZTX8; B2CT72; B2CT88; Q5IRX9;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Auxin response factor 6;
GN Name=ARF6; OrderedLocusNames=At1g30330; ORFNames=T4K22.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TRANSCRIPTIONAL ACTIVATOR.
RC STRAIN=cv. Columbia;
RX PubMed=10318972; DOI=10.1073/pnas.96.10.5844;
RA Ulmasov T., Hagen G., Guilfoyle T.J.;
RT "Activation and repression of transcription by auxin-response factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:5844-5849(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=15659631; DOI=10.1105/tpc.104.028316;
RA Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C.,
RA Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A.,
RA Yu G., Theologis A.;
RT "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family
RT members in Arabidopsis thaliana: unique and overlapping functions of ARF7
RT and ARF19.";
RL Plant Cell 17:444-463(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 793-905, AND VARIANT ASN-838.
RC STRAIN=cv. Ag-0, cv. An-1, cv. Bay-0, cv. Br-0, cv. C24, cv. Ct-1,
RC cv. Cvi-1, cv. Edi-0, cv. Ei-2, cv. Ga-0, cv. Gy-0, cv. Kas-2, cv. Ll-0,
RC cv. Mrk-0, cv. Ms-0, cv. Mt-0, cv. Nd-1, cv. Nok-3, cv. Oy-0, cv. Sorbo,
RC cv. Wa-1, cv. Wassilewskija, cv. Wei-0, and cv. Wt-5;
RX PubMed=18305205; DOI=10.1104/pp.108.116582;
RA Ehrenreich I.M., Purugganan M.D.;
RT "Sequence variation of microRNAs and their binding sites in Arabidopsis.";
RL Plant Physiol. 146:1974-1982(2008).
RN [6]
RP DIMERIZATION, AND TISSUE SPECIFICITY.
RX PubMed=10476078; DOI=10.1046/j.1365-313x.1999.00538.x;
RA Ulmasov T., Hagen G., Guilfoyle T.J.;
RT "Dimerization and DNA binding of auxin response factors.";
RL Plant J. 19:309-319(1999).
RN [7]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036261; DOI=10.1023/a:1015207114117;
RA Hagen G., Guilfoyle T.J.;
RT "Auxin-responsive gene expression: genes, promoters and regulatory
RT factors.";
RL Plant Mol. Biol. 49:373-385(2002).
RN [8]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16107481; DOI=10.1242/dev.01955;
RA Nagpal P., Ellis C.M., Weber H., Ploense S.E., Barkawi L.S.,
RA Guilfoyle T.J., Hagen G., Alonso J.M., Cohen J.D., Farmer E.E., Ecker J.R.,
RA Reed J.W.;
RT "Auxin response factors ARF6 and ARF8 promote jasmonic acid production and
RT flower maturation.";
RL Development 132:4107-4118(2005).
RN [9]
RP INDUCTION.
RX PubMed=17021043; DOI=10.1242/dev.02602;
RA Wu M.F., Tian Q., Reed J.W.;
RT "Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and
RT regulates both female and male reproduction.";
RL Development 133:4211-4218(2006).
RN [10]
RP GENE FAMILY.
RX PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA Swaminathan K., Peterson K., Jack T.;
RT "The plant B3 superfamily.";
RL Trends Plant Sci. 13:647-655(2008).
CC -!- FUNCTION: Auxin response factors (ARFs) are transcriptional factors
CC that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the
CC auxin-responsive promoter elements (AuxREs). Seems to act as
CC transcriptional activator. Formation of heterodimers with Aux/IAA
CC proteins may alter their ability to modulate early auxin response genes
CC expression. Regulates both stamen and gynoecium maturation. Promotes
CC jasmonic acid production. Partially redundant with ARF8.
CC {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481}.
CC -!- SUBUNIT: Homodimers and heterodimers. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9ZTX8; Q9XED8: ARF9; NbExp=3; IntAct=EBI-3946770, EBI-3946762;
CC Q9ZTX8; Q9XFM0: IAA28; NbExp=3; IntAct=EBI-3946770, EBI-3133404;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZTX8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZTX8-2; Sequence=VSP_037312;
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:10476078}.
CC -!- DEVELOPMENTAL STAGE: Expressed in sepals at stages 11, 12 and 13 of
CC flower development. Highly expressed in petals at stages 9-10,
CC decreases at stage 11 and disappears after flower stage 12. In anthers,
CC expressed at stage 11 in the tapetum, disappears early in stage 12 when
CC the tapetum degrades and reappears throughout the anther late in stage
CC 12 to persist at least until stage 13. In stamen filaments, expressed
CC at stages 12 to 13, especially near the apical end of the filament.
CC Expressed throughout the gynoecium at early stages up to stage 12,
CC especially strongly in ovules. Expression in gynoecium decreases late
CC in stage 12, but persists through stage 13, especially near the apical
CC end including the style. {ECO:0000269|PubMed:16107481}.
CC -!- INDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
CC -!- DOMAIN: Interactions between auxin response factors (ARFs) and Aux/IAA
CC proteins occur through their C-terminal dimerization domains III and
CC IV.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ARF family. {ECO:0000305}.
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DR EMBL; AF013467; AAD01513.1; -; mRNA.
DR EMBL; AY669788; AAT67072.1; -; mRNA.
DR EMBL; AC025295; AAG51093.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31206.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31207.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58494.1; -; Genomic_DNA.
DR EMBL; EU550049; ACB30835.1; -; Genomic_DNA.
DR EMBL; EU550050; ACB30836.1; -; Genomic_DNA.
DR EMBL; EU550051; ACB30837.1; -; Genomic_DNA.
DR EMBL; EU550052; ACB30838.1; -; Genomic_DNA.
DR EMBL; EU550053; ACB30839.1; -; Genomic_DNA.
DR EMBL; EU550054; ACB30840.1; -; Genomic_DNA.
DR EMBL; EU550055; ACB30841.1; -; Genomic_DNA.
DR EMBL; EU550056; ACB30842.1; -; Genomic_DNA.
DR EMBL; EU550057; ACB30843.1; -; Genomic_DNA.
DR EMBL; EU550058; ACB30844.1; -; Genomic_DNA.
DR EMBL; EU550059; ACB30845.1; -; Genomic_DNA.
DR EMBL; EU550060; ACB30846.1; -; Genomic_DNA.
DR EMBL; EU550061; ACB30847.1; -; Genomic_DNA.
DR EMBL; EU550062; ACB30848.1; -; Genomic_DNA.
DR EMBL; EU550063; ACB30849.1; -; Genomic_DNA.
DR EMBL; EU550064; ACB30850.1; -; Genomic_DNA.
DR EMBL; EU550065; ACB30851.1; -; Genomic_DNA.
DR EMBL; EU550066; ACB30852.1; -; Genomic_DNA.
DR EMBL; EU550067; ACB30853.1; -; Genomic_DNA.
DR EMBL; EU550068; ACB30854.1; -; Genomic_DNA.
DR EMBL; EU550069; ACB30855.1; -; Genomic_DNA.
DR EMBL; EU550070; ACB30856.1; -; Genomic_DNA.
DR EMBL; EU550071; ACB30857.1; -; Genomic_DNA.
DR EMBL; EU550072; ACB30858.1; -; Genomic_DNA.
DR PIR; F86427; F86427.
DR RefSeq; NP_001031115.1; NM_001036038.2. [Q9ZTX8-1]
DR RefSeq; NP_001320924.1; NM_001332891.1. [Q9ZTX8-1]
DR RefSeq; NP_174323.1; NM_102771.4. [Q9ZTX8-2]
DR AlphaFoldDB; Q9ZTX8; -.
DR SMR; Q9ZTX8; -.
DR BioGRID; 25148; 37.
DR IntAct; Q9ZTX8; 32.
DR STRING; 3702.AT1G30330.2; -.
DR PaxDb; Q9ZTX8; -.
DR PRIDE; Q9ZTX8; -.
DR ProteomicsDB; 240607; -. [Q9ZTX8-1]
DR EnsemblPlants; AT1G30330.1; AT1G30330.1; AT1G30330. [Q9ZTX8-2]
DR EnsemblPlants; AT1G30330.2; AT1G30330.2; AT1G30330. [Q9ZTX8-1]
DR EnsemblPlants; AT1G30330.3; AT1G30330.3; AT1G30330. [Q9ZTX8-1]
DR GeneID; 839913; -.
DR Gramene; AT1G30330.1; AT1G30330.1; AT1G30330. [Q9ZTX8-2]
DR Gramene; AT1G30330.2; AT1G30330.2; AT1G30330. [Q9ZTX8-1]
DR Gramene; AT1G30330.3; AT1G30330.3; AT1G30330. [Q9ZTX8-1]
DR KEGG; ath:AT1G30330; -.
DR Araport; AT1G30330; -.
DR TAIR; locus:2204237; AT1G30330.
DR eggNOG; ENOG502QSCZ; Eukaryota.
DR HOGENOM; CLU_002626_4_0_1; -.
DR InParanoid; Q9ZTX8; -.
DR OMA; QLMWLRG; -.
DR OrthoDB; 162380at2759; -.
DR PhylomeDB; Q9ZTX8; -.
DR PRO; PR:Q9ZTX8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ZTX8; baseline and differential.
DR Genevisible; Q9ZTX8; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IGI:TAIR.
DR GO; GO:0009733; P:response to auxin; IMP:TAIR.
DR CDD; cd10017; B3_DNA; 1.
DR Gene3D; 2.40.330.10; -; 1.
DR InterPro; IPR010525; ARF_dom.
DR InterPro; IPR044835; ARF_plant.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31384; PTHR31384; 2.
DR Pfam; PF02309; AUX_IAA; 1.
DR Pfam; PF06507; Auxin_resp; 1.
DR Pfam; PF02362; B3; 1.
DR SMART; SM01019; B3; 1.
DR SUPFAM; SSF101936; SSF101936; 1.
DR PROSITE; PS50863; B3; 1.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Auxin signaling pathway; DNA-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..935
FT /note="Auxin response factor 6"
FT /id="PRO_0000111510"
FT DOMAIN 796..880
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT DNA_BIND 129..231
FT /note="TF-B3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT REGION 536..624
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 645..714
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 896..917
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 645..691
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 896..914
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 15..16
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10318972"
FT /id="VSP_037312"
FT VARIANT 838
FT /note="D -> N (in strain: cv. Nd-1)"
FT /evidence="ECO:0000269|PubMed:18305205"
SQ SEQUENCE 935 AA; 103257 MW; 8A7A1621A0E934E3 CRC64;
MRLSSAGFNP QPHEVTGEKR VLNSELWHAC AGPLVSLPPV GSRVVYFPQG HSEQVAASTN
KEVDAHIPNY PSLHPQLICQ LHNVTMHADV ETDEVYAQMT LQPLNAQEQK DPYLPAELGV
PSRQPTNYFC KTLTASDTST HGGFSVPRRA AEKVFPPLDY SQQPPAQELM ARDLHDNEWK
FRHIFRGQPK RHLLTTGWSV FVSAKRLVAG DSVLFIWNDK NQLLLGIRRA NRPQTVMPSS
VLSSDSMHLG LLAAAAHAAA TNSRFTIFYN PRASPSEFVI PLAKYVKAVY HTRVSVGMRF
RMLFETEESS VRRYMGTITG ICDLDPTRWA NSHWRSVKVG WDESTAGERQ PRVSLWEIEP
LTTFPMYPSP FPLRLKRPWP PGLPSFHGLK EDDMGMSMSS PLMWDRGLQS LNFQGMGVNP
WMQPRLDTSG LLGMQNDVYQ AMAAAALQDM RGIDPAKAAA SLLQFQNSPG FSMQSPSLVQ
PQMLQQQLSQ QQQQLSQQQQ QQQQLSQQQQ QQLSQQQQQQ LSQQQQQQLS QQQQQQAYLG
VPETHQPQSQ AQSQSNNHLS QQQQQVVDNH NPSASSAAVV SAMSQFGSAS QPNTSPLQSM
TSLCHQQSFS DTNGGNNPIS PLHTLLSNFS QDESSQLLHL TRTNSAMTSS GWPSKRPAVD
SSFQHSGAGN NNTQSVLEQL GQSHTSNVPP NAVSLPPFPG GRECSIEQEG SASDPHSHLL
FGVNIDSSSL LMPNGMSNLR SIGIEGGDST TLPFTSSNFN NDFSGNLAMT TPSSCIDESG
FLQSSENLGS ENPQSNTFVK VYKSGSFGRS LDISKFSSYH ELRSELARMF GLEGQLEDPV
RSGWQLVFVD RENDVLLLGD DPWPEFVSSV WCIKILSPQE VQQMGKRGLE LLNSAPSSNN
VDKLPSNGNC DDFGNRSDPR NLGNGIASVG GSFNY
