ID   LES1_SCHPO              Reviewed;         431 AA.
AC   O94559;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Nuclear bridge Ish domain protein les1 {ECO:0000303|PubMed:32848252};
DE   AltName: Full=Lem-like enriched in stalks protein 1 {ECO:0000303|PubMed:32848252};
DE   Flags: Precursor;
GN   Name=les1 {ECO:0000303|PubMed:32848252}; ORFNames=SPAC23C4.05c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=32848252; DOI=10.1038/s41586-020-2648-3;
RA   Dey G., Culley S., Curran S., Schmidt U., Henriques R., Kukulski W.,
RA   Baum B.;
RT   "Closed mitosis requires local disassembly of the nuclear envelope.";
RL   Nature 585:119-123(2020).
CC   -!- FUNCTION: Inner nuclear envelope protein involved in nuclear fission,
CC       which is achieved via local disassembly of nuclear pores within the
CC       narrow bridge that links segregating daughter nuclei (PubMed:32848252).
CC       Les1 restricts the process of local nuclear envelope breakdown to the
CC       bridge midzone to prevent the leakage of material from daughter nuclei
CC       during mitosis (PubMed:32848252). {ECO:0000269|PubMed:32848252}.
CC   -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC       {ECO:0000269|PubMed:32848252}. Note=Concentrates at the stalk of each
CC       daughter nucleus during anaphase. {ECO:0000269|PubMed:32848252}.
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DR   EMBL; CU329670; CAB16876.1; -; Genomic_DNA.
DR   PIR; T38260; T38260.
DR   RefSeq; NP_593177.1; NM_001018573.2.
DR   AlphaFoldDB; O94559; -.
DR   BioGRID; 278441; 7.
DR   STRING; 4896.SPAC23C4.05c.1; -.
DR   SwissPalm; O94559; -.
DR   MaxQB; O94559; -.
DR   PaxDb; O94559; -.
DR   EnsemblFungi; SPAC23C4.05c.1; SPAC23C4.05c.1:pep; SPAC23C4.05c.
DR   GeneID; 2541954; -.
DR   KEGG; spo:SPAC23C4.05c; -.
DR   PomBase; SPAC23C4.05c; -.
DR   VEuPathDB; FungiDB:SPAC23C4.05c; -.
DR   HOGENOM; CLU_636407_0_0_1; -.
DR   InParanoid; O94559; -.
DR   OMA; KEIIHLW; -.
DR   PhylomeDB; O94559; -.
DR   PRO; PR:O94559; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0140511; C:mitotic nuclear bridge stalk; EXP:PomBase.
DR   GO; GO:0005637; C:nuclear inner membrane; EXP:PomBase.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0140515; P:mitotic nuclear bridge organization; IMP:PomBase.
DR   GO; GO:1905557; P:regulation of mitotic nuclear envelope disassembly; IMP:PomBase.
DR   InterPro; IPR018803; Ish1/Msc1-like.
DR   Pfam; PF10281; Ish1; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Membrane; Mitosis; Nucleus; Reference proteome;
KW   Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..431
FT                   /note="Nuclear bridge Ish domain protein les1"
FT                   /id="PRO_0000373865"
SQ   SEQUENCE   431 AA;  49532 MW;  E76BFDED686902D5 CRC64;
     MQPRFLLHGA LLALGIQLCL SIGKITGHIS SIEATAADIH DAPSTTTKYV QRTVYAGREK
     GKIGGPADSW PQRKLDDFLQ NHGVKSLDVP PIETPSQFWR KPLQYVSKVT DKCKSFYEKE
     KNHASHNAQK LDVWIFNSWT NSELSRWLIK NKYEVPEPGT REQLLETVFQ ASMGDAISTN
     DELESWSNNL LLSMLDQKNI TVPIGASHDD LIVLARRYYD IEERKSQDKV TNITDSQPAP
     YMKEIIHLWS DGRLIDFLRE RNIPISVLSP RETLLKEAYA NRFTPRVMIA SNVLDGWSSE
     DLLDWIWKYN KRGSIFSHVA YNSRHELIHA AKLFYMDVAS EWSSSDMASL NDSLYSHPSV
     SKQSTWTEEE LKEELESFGE LVPVPFSSTK AFERLLPHLY YYLRGPAFLN RIHYWQTFLG
     NSLKRAFVLS Q