LEU11_RALSO
ID LEU11_RALSO Reviewed; 513 AA.
AC Q8XXP1;
DT 26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=2-isopropylmalate synthase 1;
DE EC=2.3.3.13;
DE AltName: Full=Alpha-IPM synthase 1;
DE AltName: Full=Alpha-isopropylmalate synthase 1;
GN Name=leuA1; OrderedLocusNames=RSc2072; ORFNames=RS03637;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
CC -!- FUNCTION: Catalyzes the condensation of the acetyl group of acetyl-CoA
CC with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-
CC hydroxy-4-methylpentanoate (2-isopropylmalate). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-
CC isopropylmalate + CoA + H(+); Xref=Rhea:RHEA:21524, ChEBI:CHEBI:1178,
CC ChEBI:CHEBI:11851, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.13;
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 1/4.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC family. LeuA type 1 subfamily. {ECO:0000305}.
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DR EMBL; AL646052; CAD15779.1; -; Genomic_DNA.
DR RefSeq; WP_011002004.1; NC_003295.1.
DR AlphaFoldDB; Q8XXP1; -.
DR SMR; Q8XXP1; -.
DR STRING; 267608.RSc2072; -.
DR EnsemblBacteria; CAD15779; CAD15779; RSc2072.
DR GeneID; 60501584; -.
DR KEGG; rso:RSc2072; -.
DR eggNOG; COG0119; Bacteria.
DR HOGENOM; CLU_022158_0_1_4; -.
DR OMA; NTMRMLV; -.
DR UniPathway; UPA00048; UER00070.
DR Proteomes; UP000001436; Chromosome.
DR GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.20.20.70; -; 1.
DR Gene3D; 3.30.160.270; -; 1.
DR HAMAP; MF_01025; LeuA_type1; 1.
DR InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR036230; LeuA_allosteric_dom_sf.
DR InterPro; IPR005671; LeuA_bact_synth.
DR InterPro; IPR000891; PYR_CT.
DR Pfam; PF00682; HMGL-like; 1.
DR Pfam; PF08502; LeuA_dimer; 1.
DR SMART; SM00917; LeuA_dimer; 1.
DR SUPFAM; SSF110921; SSF110921; 1.
DR TIGRFAMs; TIGR00973; leuA_bact; 1.
DR PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR PROSITE; PS50991; PYR_CT; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW Leucine biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..513
FT /note="2-isopropylmalate synthase 1"
FT /id="PRO_0000140372"
FT DOMAIN 5..268
FT /note="Pyruvate carboxyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
SQ SEQUENCE 513 AA; 55573 MW; 62A68CAE3E66C0C2 CRC64;
MSDKLIIFDT TLRDGEQSPG ASMTKEEKIR IAKQLERLKV DVIEAGFAAS SNGDFEAIRA
IAQSVKDSRI CSLARANDRD ISRAAEALKP AGQFRIHTFI ATSALHMEKK LRMTPDQVYE
QARLAVRFAR QFTDDIEFSP EDGSRSDMDF LCRVLEGVIA EGATTINLPD TVGYAVPEGY
AALIRSVRER IPNSDKAIWS VHCHNDLGMA VANSLAAVKL GGARQIECTI NGLGERAGNT
SLEEVVMAVK TRRDYFNLDV GVDTTQIVPA SKLVSQITGF VVQPNKAVVG ANAFAHASGI
HQDGVLKARD TYEIMRAEDV GWTANKIVLG KLSGRNAFKQ RLQELGIELD SEAELNAAFT
RFKELADQKA EIFDEDIVAI VSNEAQHAEG EHFRFVSLSQ RSETGERPHA RIVFVADGKE
VTGEAEGNGP VDATLNAIES KVASGAEQLL YSVNAITTGT QAQGEVTVRL SKSGRIVNGV
GTDPDIVAAS AKAYLAALNK LQDKSSEKLN PQI