ARFH_ARATH
ID ARFH_ARATH Reviewed; 811 AA.
AC Q9FGV1; A8MQI4; Q5IRX7; Q9ZTR9;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Auxin response factor 8;
DE AltName: Full=Protein FRUIT WITHOUT FERTILIZATION;
GN Name=ARF8; Synonyms=FWF; OrderedLocusNames=At5g37020; ORFNames=K15O15.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TRANSCRIPTIONAL ACTIVATOR.
RC STRAIN=cv. Columbia;
RX PubMed=10318972; DOI=10.1073/pnas.96.10.5844;
RA Ulmasov T., Hagen G., Guilfoyle T.J.;
RT "Activation and repression of transcription by auxin-response factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:5844-5849(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=15659631; DOI=10.1105/tpc.104.028316;
RA Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C.,
RA Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A.,
RA Yu G., Theologis A.;
RT "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family
RT members in Arabidopsis thaliana: unique and overlapping functions of ARF7
RT and ARF19.";
RL Plant Cell 17:444-463(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP DIMERIZATION, AND TISSUE SPECIFICITY.
RX PubMed=10476078; DOI=10.1046/j.1365-313x.1999.00538.x;
RA Ulmasov T., Hagen G., Guilfoyle T.J.;
RT "Dimerization and DNA binding of auxin response factors.";
RL Plant J. 19:309-319(1999).
RN [6]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036261; DOI=10.1023/a:1015207114117;
RA Hagen G., Guilfoyle T.J.;
RT "Auxin-responsive gene expression: genes, promoters and regulatory
RT factors.";
RL Plant Mol. Biol. 49:373-385(2002).
RN [7]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16107481; DOI=10.1242/dev.01955;
RA Nagpal P., Ellis C.M., Weber H., Ploense S.E., Barkawi L.S.,
RA Guilfoyle T.J., Hagen G., Alonso J.M., Cohen J.D., Farmer E.E., Ecker J.R.,
RA Reed J.W.;
RT "Auxin response factors ARF6 and ARF8 promote jasmonic acid production and
RT flower maturation.";
RL Development 132:4107-4118(2005).
RN [8]
RP INDUCTION.
RX PubMed=17021043; DOI=10.1242/dev.02602;
RA Wu M.F., Tian Q., Reed J.W.;
RT "Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and
RT regulates both female and male reproduction.";
RL Development 133:4211-4218(2006).
RN [9]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=16829592; DOI=10.1105/tpc.105.037192;
RA Goetz M., Vivian-Smith A., Johnson S.D., Koltunow A.M.;
RT "AUXIN RESPONSE FACTOR8 is a negative regulator of fruit initiation in
RT Arabidopsis.";
RL Plant Cell 18:1873-1886(2006).
RN [10]
RP GENE FAMILY.
RX PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA Swaminathan K., Peterson K., Jack T.;
RT "The plant B3 superfamily.";
RL Trends Plant Sci. 13:647-655(2008).
CC -!- FUNCTION: Auxin response factors (ARFs) are transcriptional factors
CC that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the
CC auxin-responsive promoter elements (AuxREs). Seems to act as
CC transcriptional activator. Formation of heterodimers with Aux/IAA
CC proteins may alter their ability to modulate early auxin response genes
CC expression. Regulates both stamen and gynoecium maturation. Promotes
CC jasmonic acid production. Partially redundant with ARF6. Involved in
CC fruit initiation. Acts as an inhibitor to stop further carpel
CC development in the absence of fertilization and the generation of
CC signals required to initiate fruit and seed development.
CC {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481,
CC ECO:0000269|PubMed:16829592}.
CC -!- SUBUNIT: Homodimers and heterodimers. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9FGV1; O24409: IAA19; NbExp=4; IntAct=EBI-1554169, EBI-632257;
CC Q9FGV1; P49678: IAA2; NbExp=3; IntAct=EBI-1554169, EBI-632343;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9FGV1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FGV1-2; Sequence=VSP_037313;
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:10476078}.
CC -!- DEVELOPMENTAL STAGE: Expressed in sepals at stages 11, 12 and 13 of
CC flower development. Highly expressed in petals at stages 9-10,
CC decreases at stage 11 and disappears after flower stage 12. In anthers,
CC expressed at stage 11 in the tapetum, disappears early in stage 12 when
CC the tapetum degrades and reappears throughout the anther late in stage
CC 12 to persist at least until stage 13. In stamen filaments, expressed
CC at stages 12 to 13, especially near the apical end of the filament.
CC Expressed throughout the gynoecium at early stages up to stage 12,
CC especially strongly in ovules. Expression in gynoecium decreases late
CC in stage 12, but persists through stage 13, especially near the apical
CC end including the style. Expressed in the mesocarp of the fruit and the
CC carpel septum during fruit growth. {ECO:0000269|PubMed:16107481,
CC ECO:0000269|PubMed:16829592}.
CC -!- INDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
CC -!- DOMAIN: Interactions between auxin response factors (ARFs) and Aux/IAA
CC proteins occur through their C-terminal dimerization domains III and
CC IV.
CC -!- DISRUPTION PHENOTYPE: Seedless (parthenocarpic) fruit.
CC {ECO:0000269|PubMed:16829592}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ARF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08972.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF042196; AAD02219.1; -; mRNA.
DR EMBL; AY669790; AAT67074.1; -; mRNA.
DR EMBL; AB024026; BAB08972.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94137.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94138.1; -; Genomic_DNA.
DR RefSeq; NP_001078672.1; NM_001085203.1. [Q9FGV1-2]
DR RefSeq; NP_198518.1; NM_123060.3. [Q9FGV1-1]
DR AlphaFoldDB; Q9FGV1; -.
DR SMR; Q9FGV1; -.
DR BioGRID; 18923; 31.
DR IntAct; Q9FGV1; 26.
DR STRING; 3702.AT5G37020.1; -.
DR PaxDb; Q9FGV1; -.
DR PRIDE; Q9FGV1; -.
DR ProteomicsDB; 245183; -. [Q9FGV1-1]
DR EnsemblPlants; AT5G37020.1; AT5G37020.1; AT5G37020. [Q9FGV1-1]
DR EnsemblPlants; AT5G37020.2; AT5G37020.2; AT5G37020. [Q9FGV1-2]
DR GeneID; 833672; -.
DR Gramene; AT5G37020.1; AT5G37020.1; AT5G37020. [Q9FGV1-1]
DR Gramene; AT5G37020.2; AT5G37020.2; AT5G37020. [Q9FGV1-2]
DR KEGG; ath:AT5G37020; -.
DR Araport; AT5G37020; -.
DR TAIR; locus:2152642; AT5G37020.
DR eggNOG; ENOG502QPWF; Eukaryota.
DR InParanoid; Q9FGV1; -.
DR PhylomeDB; Q9FGV1; -.
DR PRO; PR:Q9FGV1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FGV1; baseline and differential.
DR Genevisible; Q9FGV1; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IGI:TAIR.
DR GO; GO:0010154; P:fruit development; IMP:TAIR.
DR GO; GO:0009733; P:response to auxin; IMP:TAIR.
DR CDD; cd10017; B3_DNA; 1.
DR Gene3D; 2.40.330.10; -; 1.
DR InterPro; IPR010525; ARF_dom.
DR InterPro; IPR044835; ARF_plant.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003340; B3_DNA-bd.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31384; PTHR31384; 1.
DR Pfam; PF02309; AUX_IAA; 1.
DR Pfam; PF06507; Auxin_resp; 1.
DR Pfam; PF02362; B3; 1.
DR SMART; SM01019; B3; 1.
DR SUPFAM; SSF101936; SSF101936; 1.
DR PROSITE; PS50863; B3; 1.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Auxin signaling pathway; DNA-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..811
FT /note="Auxin response factor 8"
FT /id="PRO_0000111512"
FT DOMAIN 705..789
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT DNA_BIND 126..228
FT /note="TF-B3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
FT REGION 467..496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 544..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 774..811
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_037313"
SQ SEQUENCE 811 AA; 90148 MW; 730564020F8BB134 CRC64;
MKLSTSGLGQ QGHEGEKCLN SELWHACAGP LVSLPSSGSR VVYFPQGHSE QVAATTNKEV
DGHIPNYPSL PPQLICQLHN VTMHADVETD EVYAQMTLQP LTPEEQKETF VPIELGIPSK
QPSNYFCKTL TASDTSTHGG FSVPRRAAEK VFPPLDYTLQ PPAQELIARD LHDVEWKFRH
IFRGQPKRHL LTTGWSVFVS AKRLVAGDSV IFIRNEKNQL FLGIRHATRP QTIVPSSVLS
SDSMHIGLLA AAAHASATNS CFTVFFHPRA SQSEFVIQLS KYIKAVFHTR ISVGMRFRML
FETEESSVRR YMGTITGISD LDSVRWPNSH WRSVKVGWDE STAGERQPRV SLWEIEPLTT
FPMYPSLFPL RLKRPWHAGT SSLPDGRGDL GSGLTWLRGG GGEQQGLLPL NYPSVGLFPW
MQQRLDLSQM GTDNNQQYQA MLAAGLQNIG GGDPLRQQFV QLQEPHHQYL QQSASHNSDL
MLQQQQQQQA SRHLMHAQTQ IMSENLPQQN MRQEVSNQPA GQQQQLQQPD QNAYLNAFKM
QNGHLQQWQQ QSEMPSPSFM KSDFTDSSNK FATTASPASG DGNLLNFSIT GQSVLPEQLT
TEGWSPKASN TFSEPLSLPQ AYPGKSLALE PGNPQNPSLF GVDPDSGLFL PSTVPRFASS
SGDAEASPMS LTDSGFQNSL YSCMQDTTHE LLHGAGQINS SNQTKNFVKV YKSGSVGRSL
DISRFSSYHE LREELGKMFA IEGLLEDPLR SGWQLVFVDK ENDILLLGDD PWESFVNNVW
YIKILSPEDV HQMGDHGEGS GGLFPQNPTH L
